Tags

Type your tag names separated by a space and hit enter

Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation.
Ups J Med Sci. 2013 Aug; 118(3):153-64.UJ

Abstract

BACKGROUND

Glycosylation and glycan composition are of fundamental importance for the biological properties of FSH and LH. The aim of this study was to determine the glycosylation, sialylation, and sulfonation of serum FSH and LH throughout the normal menstrual cycle.

METHODS

Serum samples were collected from 79 healthy women with regular menstrual cycles. The mean numbers of anionic monosaccharide (AMS), sialic acid (SA), and sulfonated N-acetylgalactosamine (SU) residues per FSH and LH molecule were estimated for all sera with methods based on electrophoreses, neuraminidase treatments, and fluoroimmunoassays of the gonadotrophins.

RESULTS

Di-glycosylated glycoforms (FSHdi, LHdi) were detected in serum in addition to tetra-glycosylated FSH (FSHtetra) and tri-glycosylated LH (LHtri). FSHdi exhibited two peaks: one on day 5 to 7 and one, more pronounced, at midcycle. FSHtetra plateaued at a high concentration from day 5 to 15, without a midcycle peak. There were lower concentrations of LHdi than LHtri, except at midcycle when the opposite occurred. The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle. The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped.

CONCLUSION

Serum FSH and LH governing the natural ovarian stimulation process exhibited dynamic changes of glycosylation and glycan composition. This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women.

Authors+Show Affiliations

Department of Medical Sciences, Uppsala University, Clinical Chemistry, University Hospital, SE 751 85 Uppsala, Sweden. leif.wide@medsci.uu.seNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23527482

Citation

Wide, Leif, and Karin Eriksson. "Dynamic Changes in Glycosylation and Glycan Composition of Serum FSH and LH During Natural Ovarian Stimulation." Upsala Journal of Medical Sciences, vol. 118, no. 3, 2013, pp. 153-64.
Wide L, Eriksson K. Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation. Ups J Med Sci. 2013;118(3):153-64.
Wide, L., & Eriksson, K. (2013). Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation. Upsala Journal of Medical Sciences, 118(3), 153-64. https://doi.org/10.3109/03009734.2013.782081
Wide L, Eriksson K. Dynamic Changes in Glycosylation and Glycan Composition of Serum FSH and LH During Natural Ovarian Stimulation. Ups J Med Sci. 2013;118(3):153-64. PubMed PMID: 23527482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation. AU - Wide,Leif, AU - Eriksson,Karin, Y1 - 2013/03/25/ PY - 2013/3/27/entrez PY - 2013/3/27/pubmed PY - 2014/1/28/medline SP - 153 EP - 64 JF - Upsala journal of medical sciences JO - Ups J Med Sci VL - 118 IS - 3 N2 - BACKGROUND: Glycosylation and glycan composition are of fundamental importance for the biological properties of FSH and LH. The aim of this study was to determine the glycosylation, sialylation, and sulfonation of serum FSH and LH throughout the normal menstrual cycle. METHODS: Serum samples were collected from 79 healthy women with regular menstrual cycles. The mean numbers of anionic monosaccharide (AMS), sialic acid (SA), and sulfonated N-acetylgalactosamine (SU) residues per FSH and LH molecule were estimated for all sera with methods based on electrophoreses, neuraminidase treatments, and fluoroimmunoassays of the gonadotrophins. RESULTS: Di-glycosylated glycoforms (FSHdi, LHdi) were detected in serum in addition to tetra-glycosylated FSH (FSHtetra) and tri-glycosylated LH (LHtri). FSHdi exhibited two peaks: one on day 5 to 7 and one, more pronounced, at midcycle. FSHtetra plateaued at a high concentration from day 5 to 15, without a midcycle peak. There were lower concentrations of LHdi than LHtri, except at midcycle when the opposite occurred. The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle. The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped. CONCLUSION: Serum FSH and LH governing the natural ovarian stimulation process exhibited dynamic changes of glycosylation and glycan composition. This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women. SN - 2000-1967 UR - https://www.unboundmedicine.com/medline/citation/23527482/Dynamic_changes_in_glycosylation_and_glycan_composition_of_serum_FSH_and_LH_during_natural_ovarian_stimulation_ L2 - https://www.tandfonline.com/doi/full/10.3109/03009734.2013.782081 DB - PRIME DP - Unbound Medicine ER -