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Diallyl sulfide promotes cell-cycle arrest through the p53 expression and triggers induction of apoptosis via caspase- and mitochondria-dependent signaling pathways in human cervical cancer Ca Ski cells.
Nutr Cancer. 2013; 65(3):505-14.NC

Abstract

Diallyl sulfide (DAS) is a component of garlic (Alliaceae family). Although diallyl polysulfide has been shown to exhibit anticancer activities, no report explored DAS-affected cell death in human cervical cancer cells in vitro. This study investigated DAS affected on cell-cycle regulation and apoptosis in human cervical cancer Ca Ski cells. DAS at 25-100 μM decreased the viability of Ca Ski cells by increasing G0/G1 phase arrest followed by induction of apoptosis in concentration- and time-dependent effects. Flow cytomteric assay indicated that DAS (75 μM) promoted the production of Ca(2+) accumulation and decreased the level of mitochondrial membrane potential in Ca Ski cells. Western blotting showed that 75 μM of DAS-induced G0/G1 phase arrest was mediated through the increased expression of p21, p27, and p53 with a simultaneous decrease in CDK2, CDK6, and CHK2 expression. The characteristics of apoptosis, such as morphological changes and DNA condensation, altered the ratio of Bax/Bcl-2 and sub-G1 phase occurred in Ca Ski cells after exposure to DAS. Furthermore, DAS induced mitochondrial dysfunction, leading to the release of cytochrome c for causing apoptosis in Ca Ski cells. These findings suggest that DAS might be a potential chemotherapeutic agent for the treatment of cervical cancer.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, China Medical University Hospital, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23530650

Citation

Chiu, Tsan-Hung, et al. "Diallyl Sulfide Promotes Cell-cycle Arrest Through the P53 Expression and Triggers Induction of Apoptosis Via Caspase- and Mitochondria-dependent Signaling Pathways in Human Cervical Cancer Ca Ski Cells." Nutrition and Cancer, vol. 65, no. 3, 2013, pp. 505-14.
Chiu TH, Lan KY, Yang MD, et al. Diallyl sulfide promotes cell-cycle arrest through the p53 expression and triggers induction of apoptosis via caspase- and mitochondria-dependent signaling pathways in human cervical cancer Ca Ski cells. Nutr Cancer. 2013;65(3):505-14.
Chiu, T. H., Lan, K. Y., Yang, M. D., Lin, J. J., Hsia, T. C., Wu, C. T., Yang, J. S., Chueh, F. S., & Chung, J. G. (2013). Diallyl sulfide promotes cell-cycle arrest through the p53 expression and triggers induction of apoptosis via caspase- and mitochondria-dependent signaling pathways in human cervical cancer Ca Ski cells. Nutrition and Cancer, 65(3), 505-14. https://doi.org/10.1080/01635581.2012.725503
Chiu TH, et al. Diallyl Sulfide Promotes Cell-cycle Arrest Through the P53 Expression and Triggers Induction of Apoptosis Via Caspase- and Mitochondria-dependent Signaling Pathways in Human Cervical Cancer Ca Ski Cells. Nutr Cancer. 2013;65(3):505-14. PubMed PMID: 23530650.
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TY - JOUR T1 - Diallyl sulfide promotes cell-cycle arrest through the p53 expression and triggers induction of apoptosis via caspase- and mitochondria-dependent signaling pathways in human cervical cancer Ca Ski cells. AU - Chiu,Tsan-Hung, AU - Lan,Kai-Ying, AU - Yang,Mei-Due, AU - Lin,Jen-Jyh, AU - Hsia,Te-Chun, AU - Wu,Chin-Tung, AU - Yang,Jai-Sing, AU - Chueh,Fu-Shin, AU - Chung,Jing-Gung, PY - 2013/3/28/entrez PY - 2013/3/28/pubmed PY - 2013/9/17/medline SP - 505 EP - 14 JF - Nutrition and cancer JO - Nutr Cancer VL - 65 IS - 3 N2 - Diallyl sulfide (DAS) is a component of garlic (Alliaceae family). Although diallyl polysulfide has been shown to exhibit anticancer activities, no report explored DAS-affected cell death in human cervical cancer cells in vitro. This study investigated DAS affected on cell-cycle regulation and apoptosis in human cervical cancer Ca Ski cells. DAS at 25-100 μM decreased the viability of Ca Ski cells by increasing G0/G1 phase arrest followed by induction of apoptosis in concentration- and time-dependent effects. Flow cytomteric assay indicated that DAS (75 μM) promoted the production of Ca(2+) accumulation and decreased the level of mitochondrial membrane potential in Ca Ski cells. Western blotting showed that 75 μM of DAS-induced G0/G1 phase arrest was mediated through the increased expression of p21, p27, and p53 with a simultaneous decrease in CDK2, CDK6, and CHK2 expression. The characteristics of apoptosis, such as morphological changes and DNA condensation, altered the ratio of Bax/Bcl-2 and sub-G1 phase occurred in Ca Ski cells after exposure to DAS. Furthermore, DAS induced mitochondrial dysfunction, leading to the release of cytochrome c for causing apoptosis in Ca Ski cells. These findings suggest that DAS might be a potential chemotherapeutic agent for the treatment of cervical cancer. SN - 1532-7914 UR - https://www.unboundmedicine.com/medline/citation/23530650/Diallyl_sulfide_promotes_cell_cycle_arrest_through_the_p53_expression_and_triggers_induction_of_apoptosis_via_caspase__and_mitochondria_dependent_signaling_pathways_in_human_cervical_cancer_Ca_Ski_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/01635581.2012.725503 DB - PRIME DP - Unbound Medicine ER -