Tags

Type your tag names separated by a space and hit enter

Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves.
BMC Res Notes. 2013 Mar 27; 6:121.BR

Abstract

BACKGROUND

Research on natural products has gained a wide popularity due to the potential of discovering active compounds. The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves.

METHODS

Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents.

RESULTS

Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18 ± 18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC(50) value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC(50) 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE > APD > APM > APA > APC.

CONCLUSION

The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.

Authors+Show Affiliations

Department of Pharmacy, University of Rajshahi, Rajshahi-6205, Bangladesh.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23531304

Citation

khatoon, Mahfuza, et al. "Estimation of Total Phenol and in Vitro Antioxidant Activity of Albizia Procera Leaves." BMC Research Notes, vol. 6, 2013, p. 121.
khatoon M, Islam E, Islam R, et al. Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Res Notes. 2013;6:121.
khatoon, M., Islam, E., Islam, R., Rahman, A. A., Alam, A. H., Khondkar, P., Rashid, M., & Parvin, S. (2013). Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. BMC Research Notes, 6, 121. https://doi.org/10.1186/1756-0500-6-121
khatoon M, et al. Estimation of Total Phenol and in Vitro Antioxidant Activity of Albizia Procera Leaves. BMC Res Notes. 2013 Mar 27;6:121. PubMed PMID: 23531304.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Estimation of total phenol and in vitro antioxidant activity of Albizia procera leaves. AU - khatoon,Mahfuza, AU - Islam,Ekramul, AU - Islam,Rafikul, AU - Rahman,Aziz Abdur, AU - Alam,A H M Khurshid, AU - Khondkar,Proma, AU - Rashid,Mamunur, AU - Parvin,Shahnaj, Y1 - 2013/03/27/ PY - 2012/11/23/received PY - 2013/03/20/accepted PY - 2013/3/28/entrez PY - 2013/3/28/pubmed PY - 2013/12/16/medline SP - 121 EP - 121 JF - BMC research notes JO - BMC Res Notes VL - 6 N2 - BACKGROUND: Research on natural products has gained a wide popularity due to the potential of discovering active compounds. The antioxidant properties contained in plants have been proposed as one of the mechanisms for the observed beneficial effect. Therefore, the present study investigated the antioxidant activity and total phenolic contents of various solvent extracts of Albizia procera leaves. METHODS: Antioxidant activity of the methanol extract and its derived fractions petroleum ether (APP), carbon tetrachloride (APC), dichloromethane (APD), ethyl acetate (APE), and residual aqueous fraction (APA) of the leaves of Albizia procera was performed by in vitro chemical analyses. Total phenolic content of the APM and other five fractions were also determined. APM and its derived fractions were also subjected to preliminary phytochemical screening test for various constituents. RESULTS: Phytochemical screening revealed the presence of saponins, steroids, tannins, glycosides and flavonoids in the extracts. Amongst the extracts, APE showed the highest total phenolic content (449.18 ± 18.41mg of gallic acid equivalent/g of extract). In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging test, the IC(50) value of APM, APP, APC, APD, APE and APA was 43.43, 63.60, 166.18, 41.15, 11.79, and 63.06 μg/mL, respectively. Therefore, among the APM and its derived fractions, APE showed the highest antioxidant activity which is comparable to that of standard ascorbic acid (AA) (IC(50) 10.12 μg/mL). The total antioxidant capacity was found to be varied in different fractions. The reducing activity on ferrous ion was ranked as APE > APD > APM > APA > APC. CONCLUSION: The above evidences suggest that APE of A. procera leaf is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals. SN - 1756-0500 UR - https://www.unboundmedicine.com/medline/citation/23531304/Estimation_of_total_phenol_and_in_vitro_antioxidant_activity_of_Albizia_procera_leaves_ L2 - https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-6-121 DB - PRIME DP - Unbound Medicine ER -