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Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation.
J Infect Dis. 2013 Jun 01; 207(11):1743-52.JI

Abstract

The emerging novel human betacoronavirus 2c EMC/2012 (HCoV-EMC) was recently isolated from patients with severe pneumonia and renal failure and was associated with an unexplained high crude fatality rate of 56%. We performed a cell line susceptibility study with 28 cell lines. HCoV-EMC was found to infect the human respiratory tract (polarized airway epithelium cell line Calu-3, embryonic fibroblast cell line HFL, and lung adenocarcinoma cell line A549), kidney (embryonic kidney cell line HEK), intestinal tract (colorectal adenocarcinoma cell line Caco-2), liver cells (hepatocellular carcinoma cell line Huh-7), and histiocytes (malignant histiocytoma cell line His-1), as evident by detection of high or increasing viral load in culture supernatants, detection of viral nucleoprotein expression by immunostaining, and/or detection of cytopathic effects. Although an infected human neuronal cell line (NT2) and infected monocyte and T lymphocyte cell lines (THP-1, U937, and H9) had increased viral loads, their relatively lower viral production corroborated with absent nucleoprotein expression and cytopathic effects. This range of human tissue tropism is broader than that for all other HCoVs, including SARS coronavirus, HCoV-OC43, HCoV-HKU1, HCoV-229E, and HCoV-NL63, which may explain the high mortality associated with this disease. A recent cell line susceptibility study showed that HCoV-EMC can infect primate, porcine, and bat cells and therefore may jump interspecies barriers. We found that HCoV-EMC can also infect civet lung fibroblast and rabbit kidney cell lines. These findings have important implications for the diagnosis, pathogenesis, and transmission of HCoV-EMC.

Authors+Show Affiliations

Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Rd, Pokfulam, Hong Kong Special Administrative Region, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23532101

Citation

Chan, Jasper Fuk-Woo, et al. "Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation." The Journal of Infectious Diseases, vol. 207, no. 11, 2013, pp. 1743-52.
Chan JF, Chan KH, Choi GK, et al. Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation. J Infect Dis. 2013;207(11):1743-52.
Chan, J. F., Chan, K. H., Choi, G. K., To, K. K., Tse, H., Cai, J. P., Yeung, M. L., Cheng, V. C., Chen, H., Che, X. Y., Lau, S. K., Woo, P. C., & Yuen, K. Y. (2013). Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation. The Journal of Infectious Diseases, 207(11), 1743-52. https://doi.org/10.1093/infdis/jit123
Chan JF, et al. Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation. J Infect Dis. 2013 Jun 1;207(11):1743-52. PubMed PMID: 23532101.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation. AU - Chan,Jasper Fuk-Woo, AU - Chan,Kwok-Hung, AU - Choi,Garnet Kwan-Yue, AU - To,Kelvin Kai-Wang, AU - Tse,Herman, AU - Cai,Jian-Piao, AU - Yeung,Man Lung, AU - Cheng,Vincent Chi-Chung, AU - Chen,Honglin, AU - Che,Xiao-Yan, AU - Lau,Susanna Kar-Pui, AU - Woo,Patrick Chiu-Yat, AU - Yuen,Kwok-Yung, Y1 - 2013/03/26/ PY - 2013/3/28/entrez PY - 2013/3/28/pubmed PY - 2013/7/3/medline SP - 1743 EP - 52 JF - The Journal of infectious diseases JO - J. Infect. Dis. VL - 207 IS - 11 N2 - The emerging novel human betacoronavirus 2c EMC/2012 (HCoV-EMC) was recently isolated from patients with severe pneumonia and renal failure and was associated with an unexplained high crude fatality rate of 56%. We performed a cell line susceptibility study with 28 cell lines. HCoV-EMC was found to infect the human respiratory tract (polarized airway epithelium cell line Calu-3, embryonic fibroblast cell line HFL, and lung adenocarcinoma cell line A549), kidney (embryonic kidney cell line HEK), intestinal tract (colorectal adenocarcinoma cell line Caco-2), liver cells (hepatocellular carcinoma cell line Huh-7), and histiocytes (malignant histiocytoma cell line His-1), as evident by detection of high or increasing viral load in culture supernatants, detection of viral nucleoprotein expression by immunostaining, and/or detection of cytopathic effects. Although an infected human neuronal cell line (NT2) and infected monocyte and T lymphocyte cell lines (THP-1, U937, and H9) had increased viral loads, their relatively lower viral production corroborated with absent nucleoprotein expression and cytopathic effects. This range of human tissue tropism is broader than that for all other HCoVs, including SARS coronavirus, HCoV-OC43, HCoV-HKU1, HCoV-229E, and HCoV-NL63, which may explain the high mortality associated with this disease. A recent cell line susceptibility study showed that HCoV-EMC can infect primate, porcine, and bat cells and therefore may jump interspecies barriers. We found that HCoV-EMC can also infect civet lung fibroblast and rabbit kidney cell lines. These findings have important implications for the diagnosis, pathogenesis, and transmission of HCoV-EMC. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/23532101/Differential_cell_line_susceptibility_to_the_emerging_novel_human_betacoronavirus_2c_EMC/2012:_implications_for_disease_pathogenesis_and_clinical_manifestation_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jit123 DB - PRIME DP - Unbound Medicine ER -