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Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation.
Nature. 2013 Apr 04; 496(7443):91-5.Nat

Abstract

About 8,000 years ago in the Fertile Crescent, a spontaneous hybridization of the wild diploid grass Aegilops tauschii (2n = 14; DD) with the cultivated tetraploid wheat Triticum turgidum (2n = 4x = 28; AABB) resulted in hexaploid wheat (T. aestivum; 2n = 6x = 42; AABBDD). Wheat has since become a primary staple crop worldwide as a result of its enhanced adaptability to a wide range of climates and improved grain quality for the production of baker's flour. Here we describe sequencing the Ae. tauschii genome and obtaining a roughly 90-fold depth of short reads from libraries with various insert sizes, to gain a better understanding of this genetically complex plant. The assembled scaffolds represented 83.4% of the genome, of which 65.9% comprised transposable elements. We generated comprehensive RNA-Seq data and used it to identify 43,150 protein-coding genes, of which 30,697 (71.1%) were uniquely anchored to chromosomes with an integrated high-density genetic map. Whole-genome analysis revealed gene family expansion in Ae. tauschii of agronomically relevant gene families that were associated with disease resistance, abiotic stress tolerance and grain quality. This draft genome sequence provides insight into the environmental adaptation of bread wheat and can aid in defining the large and complicated genomes of wheat species.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23535592

Citation

Jia, Jizeng, et al. "Aegilops Tauschii Draft Genome Sequence Reveals a Gene Repertoire for Wheat Adaptation." Nature, vol. 496, no. 7443, 2013, pp. 91-5.
Jia J, Zhao S, Kong X, et al. Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature. 2013;496(7443):91-5.
Jia, J., Zhao, S., Kong, X., Li, Y., Zhao, G., He, W., Appels, R., Pfeifer, M., Tao, Y., Zhang, X., Jing, R., Zhang, C., Ma, Y., Gao, L., Gao, C., Spannagl, M., Mayer, K. F., Li, D., Pan, S., ... Wang, J. (2013). Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature, 496(7443), 91-5. https://doi.org/10.1038/nature12028
Jia J, et al. Aegilops Tauschii Draft Genome Sequence Reveals a Gene Repertoire for Wheat Adaptation. Nature. 2013 Apr 4;496(7443):91-5. PubMed PMID: 23535592.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. AU - Jia,Jizeng, AU - Zhao,Shancen, AU - Kong,Xiuying, AU - Li,Yingrui, AU - Zhao,Guangyao, AU - He,Weiming, AU - Appels,Rudi, AU - Pfeifer,Matthias, AU - Tao,Yong, AU - Zhang,Xueyong, AU - Jing,Ruilian, AU - Zhang,Chi, AU - Ma,Youzhi, AU - Gao,Lifeng, AU - Gao,Chuan, AU - Spannagl,Manuel, AU - Mayer,Klaus F X, AU - Li,Dong, AU - Pan,Shengkai, AU - Zheng,Fengya, AU - Hu,Qun, AU - Xia,Xianchun, AU - Li,Jianwen, AU - Liang,Qinsi, AU - Chen,Jie, AU - Wicker,Thomas, AU - Gou,Caiyun, AU - Kuang,Hanhui, AU - He,Genyun, AU - Luo,Yadan, AU - Keller,Beat, AU - Xia,Qiuju, AU - Lu,Peng, AU - Wang,Junyi, AU - Zou,Hongfeng, AU - Zhang,Rongzhi, AU - Xu,Junyang, AU - Gao,Jinlong, AU - Middleton,Christopher, AU - Quan,Zhiwu, AU - Liu,Guangming, AU - Wang,Jian, AU - ,, AU - Yang,Huanming, AU - Liu,Xu, AU - He,Zhonghu, AU - Mao,Long, AU - Wang,Jun, Y1 - 2013/03/24/ PY - 2012/03/28/received PY - 2013/02/20/accepted PY - 2013/3/29/entrez PY - 2013/3/29/pubmed PY - 2013/5/2/medline SP - 91 EP - 5 JF - Nature JO - Nature VL - 496 IS - 7443 N2 - About 8,000 years ago in the Fertile Crescent, a spontaneous hybridization of the wild diploid grass Aegilops tauschii (2n = 14; DD) with the cultivated tetraploid wheat Triticum turgidum (2n = 4x = 28; AABB) resulted in hexaploid wheat (T. aestivum; 2n = 6x = 42; AABBDD). Wheat has since become a primary staple crop worldwide as a result of its enhanced adaptability to a wide range of climates and improved grain quality for the production of baker's flour. Here we describe sequencing the Ae. tauschii genome and obtaining a roughly 90-fold depth of short reads from libraries with various insert sizes, to gain a better understanding of this genetically complex plant. The assembled scaffolds represented 83.4% of the genome, of which 65.9% comprised transposable elements. We generated comprehensive RNA-Seq data and used it to identify 43,150 protein-coding genes, of which 30,697 (71.1%) were uniquely anchored to chromosomes with an integrated high-density genetic map. Whole-genome analysis revealed gene family expansion in Ae. tauschii of agronomically relevant gene families that were associated with disease resistance, abiotic stress tolerance and grain quality. This draft genome sequence provides insight into the environmental adaptation of bread wheat and can aid in defining the large and complicated genomes of wheat species. SN - 1476-4687 UR - https://www.unboundmedicine.com/medline/citation/23535592/Aegilops_tauschii_draft_genome_sequence_reveals_a_gene_repertoire_for_wheat_adaptation_ L2 - https://doi.org/10.1038/nature12028 DB - PRIME DP - Unbound Medicine ER -