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Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat.
J Exp Bot. 2013 Apr; 64(7):2027-40.JE

Abstract

Low-molecular-weight glutenin subunits (LMW-GS), encoded by a complex multigene family, play an important role in the processing quality of wheat flour. Although members of this gene family have been identified in several wheat varieties, the allelic variation and composition of LMW-GS genes in common wheat are not well understood. In the present study, using the LMW-GS gene molecular marker system and the full-length gene cloning method, a comprehensive molecular analysis of LMW-GS genes was conducted in a representative population, the micro-core collections (MCC) of Chinese wheat germplasm. Generally, >15 LMW-GS genes were identified from individual MCC accessions, of which 4-6 were located at the Glu-A3 locus, 3-5 at the Glu-B3 locus, and eight at the Glu-D3 locus. LMW-GS genes at the Glu-A3 locus showed the highest allelic diversity, followed by the Glu-B3 genes, while the Glu-D3 genes were extremely conserved among MCC accessions. Expression and sequence analysis showed that 9-13 active LMW-GS genes were present in each accession. Sequence identity analysis showed that all i-type genes present at the Glu-A3 locus formed a single group, the s-type genes located at Glu-B3 and Glu-D3 loci comprised a unique group, while high-diversity m-type genes were classified into four groups and detected in all Glu-3 loci. These results contribute to the functional analysis of LMW-GS genes and facilitate improvement of bread-making quality by wheat molecular breeding programmes.

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Authors+Show Affiliations

Zhang X
State Key Laboratory of Plant Cell and Chromosome Engineering, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 1 West Beichen Road, Chaoyang District, Beijing 100101, China.
Liu D
No affiliation info available
Zhang J
No affiliation info available
Jiang W
No affiliation info available
Luo G
No affiliation info available
Yang W
No affiliation info available
Sun J
No affiliation info available
Tong Y
No affiliation info available
Cui D
No affiliation info available
Zhang A
No affiliation info available

MeSH

AllelesGene Expression Regulation, PlantGene OrderGlutensTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23536608

Citation

Zhang, Xiaofei, et al. "Novel Insights Into the Composition, Variation, Organization, and Expression of the Low-molecular-weight Glutenin Subunit Gene Family in Common Wheat." Journal of Experimental Botany, vol. 64, no. 7, 2013, pp. 2027-40.
Zhang X, Liu D, Zhang J, et al. Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. J Exp Bot. 2013;64(7):2027-40.
Zhang, X., Liu, D., Zhang, J., Jiang, W., Luo, G., Yang, W., Sun, J., Tong, Y., Cui, D., & Zhang, A. (2013). Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. Journal of Experimental Botany, 64(7), 2027-40. https://doi.org/10.1093/jxb/ert070
Zhang X, et al. Novel Insights Into the Composition, Variation, Organization, and Expression of the Low-molecular-weight Glutenin Subunit Gene Family in Common Wheat. J Exp Bot. 2013;64(7):2027-40. PubMed PMID: 23536608.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. AU - Zhang,Xiaofei, AU - Liu,Dongcheng, AU - Zhang,Jianghua, AU - Jiang,Wei, AU - Luo,Guangbin, AU - Yang,Wenlong, AU - Sun,Jiazhu, AU - Tong,Yiping, AU - Cui,Dangqun, AU - Zhang,Aimin, Y1 - 2013/03/27/ PY - 2013/3/29/entrez PY - 2013/3/29/pubmed PY - 2014/5/31/medline SP - 2027 EP - 40 JF - Journal of experimental botany JO - J Exp Bot VL - 64 IS - 7 N2 - Low-molecular-weight glutenin subunits (LMW-GS), encoded by a complex multigene family, play an important role in the processing quality of wheat flour. Although members of this gene family have been identified in several wheat varieties, the allelic variation and composition of LMW-GS genes in common wheat are not well understood. In the present study, using the LMW-GS gene molecular marker system and the full-length gene cloning method, a comprehensive molecular analysis of LMW-GS genes was conducted in a representative population, the micro-core collections (MCC) of Chinese wheat germplasm. Generally, >15 LMW-GS genes were identified from individual MCC accessions, of which 4-6 were located at the Glu-A3 locus, 3-5 at the Glu-B3 locus, and eight at the Glu-D3 locus. LMW-GS genes at the Glu-A3 locus showed the highest allelic diversity, followed by the Glu-B3 genes, while the Glu-D3 genes were extremely conserved among MCC accessions. Expression and sequence analysis showed that 9-13 active LMW-GS genes were present in each accession. Sequence identity analysis showed that all i-type genes present at the Glu-A3 locus formed a single group, the s-type genes located at Glu-B3 and Glu-D3 loci comprised a unique group, while high-diversity m-type genes were classified into four groups and detected in all Glu-3 loci. These results contribute to the functional analysis of LMW-GS genes and facilitate improvement of bread-making quality by wheat molecular breeding programmes. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/23536608/Novel_insights_into_the_composition_variation_organization_and_expression_of_the_low_molecular_weight_glutenin_subunit_gene_family_in_common_wheat_ L2 - https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert070 DB - PRIME DP - Unbound Medicine ER -