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HPLC-DAD-ESI/MS identification and quantification of phenolic compounds in Ilex paraguariensis beverages and on-line evaluation of individual antioxidant activity.
Molecules. 2013 Mar 28; 18(4):3859-71.M

Abstract

"Chimarrão" and "tererê" are maté (dried, toasted and milled Ilex paraguariensis leaves and stemlets) beverages widely consumed in South America. This paper describes the application of HPLC-DAD-ESI/MS method for the identification and quantification of caffeoylquinic acids (CQA), flavonol glycosides and purine alkaloids in these beverages. The beverage samples were prepared from commercial lots of maté from Southern Brazil. The caffeoylquinic acids, 4,5-diCQA, 3-CQA, 5-CQA, and 4-CQA were the major compounds, having 238-289, 153-242, 183-263, and 123-188 μg/mL, respectively, for chimarrão and 206-265, 122-218, 164-209, 103-169 μg/mL, respectively, for tererê. Caffeine also had high amounts while glycosides of quercetin and kaempferol were found at much lower levels. The individual antioxidant activity was also determined by an on-line system that measured their ABTS•+ radical scavenging activity, showing that the antioxidant capacity was not proportional to the concentrations of the phenolic compounds. 3-CQA, quercetina-3-O-ramnosylglucoside, and quercetina-3-O-glucoside were the major contributors to the antioxidant capacity, although the quercetin glycosides had concentrations less than 10 times that of 3-CQA.

Authors+Show Affiliations

Department of Food Science, Faculty of Food Engineering, University of Campinas-UNICAMP, P.O. Box 6121, 13083-862 Campinas, SP, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23538900

Citation

Peres, Renato G., et al. "HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex Paraguariensis Beverages and On-line Evaluation of Individual Antioxidant Activity." Molecules (Basel, Switzerland), vol. 18, no. 4, 2013, pp. 3859-71.
Peres RG, Tonin FG, Tavares MF, et al. HPLC-DAD-ESI/MS identification and quantification of phenolic compounds in Ilex paraguariensis beverages and on-line evaluation of individual antioxidant activity. Molecules. 2013;18(4):3859-71.
Peres, R. G., Tonin, F. G., Tavares, M. F., & Rodriguez-Amaya, D. B. (2013). HPLC-DAD-ESI/MS identification and quantification of phenolic compounds in Ilex paraguariensis beverages and on-line evaluation of individual antioxidant activity. Molecules (Basel, Switzerland), 18(4), 3859-71. https://doi.org/10.3390/molecules18043859
Peres RG, et al. HPLC-DAD-ESI/MS Identification and Quantification of Phenolic Compounds in Ilex Paraguariensis Beverages and On-line Evaluation of Individual Antioxidant Activity. Molecules. 2013 Mar 28;18(4):3859-71. PubMed PMID: 23538900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HPLC-DAD-ESI/MS identification and quantification of phenolic compounds in Ilex paraguariensis beverages and on-line evaluation of individual antioxidant activity. AU - Peres,Renato G, AU - Tonin,Fernando G, AU - Tavares,Marina F M, AU - Rodriguez-Amaya,Delia B, Y1 - 2013/03/28/ PY - 2012/12/27/received PY - 2013/03/19/revised PY - 2013/03/20/accepted PY - 2013/3/30/entrez PY - 2013/3/30/pubmed PY - 2013/10/18/medline SP - 3859 EP - 71 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 18 IS - 4 N2 - "Chimarrão" and "tererê" are maté (dried, toasted and milled Ilex paraguariensis leaves and stemlets) beverages widely consumed in South America. This paper describes the application of HPLC-DAD-ESI/MS method for the identification and quantification of caffeoylquinic acids (CQA), flavonol glycosides and purine alkaloids in these beverages. The beverage samples were prepared from commercial lots of maté from Southern Brazil. The caffeoylquinic acids, 4,5-diCQA, 3-CQA, 5-CQA, and 4-CQA were the major compounds, having 238-289, 153-242, 183-263, and 123-188 μg/mL, respectively, for chimarrão and 206-265, 122-218, 164-209, 103-169 μg/mL, respectively, for tererê. Caffeine also had high amounts while glycosides of quercetin and kaempferol were found at much lower levels. The individual antioxidant activity was also determined by an on-line system that measured their ABTS•+ radical scavenging activity, showing that the antioxidant capacity was not proportional to the concentrations of the phenolic compounds. 3-CQA, quercetina-3-O-ramnosylglucoside, and quercetina-3-O-glucoside were the major contributors to the antioxidant capacity, although the quercetin glycosides had concentrations less than 10 times that of 3-CQA. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/23538900/HPLC_DAD_ESI/MS_identification_and_quantification_of_phenolic_compounds_in_Ilex_paraguariensis_beverages_and_on_line_evaluation_of_individual_antioxidant_activity_ L2 - https://www.mdpi.com/resolver?pii=molecules18043859 DB - PRIME DP - Unbound Medicine ER -