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Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats.
Clin Nutr. 2013 Dec; 32(6):993-8.CN

Abstract

BACKGROUND & AIMS

The importance of route of administration of omega-3 (n-3) polyunsaturated fatty acids (PUFA) (oral vs intravenous (iv)) is not clear. We determined the relative concentrations of fatty acids in plasma phosphatidylcholine (PC), red blood cells (RBC), white blood cells (WBC) and several tissues after short-term oral or iv administration of soybean oil (SO) or fish oil (FO).

METHODS

Wistar rats (n = 6/group) received saline, FO, or SO by gavage or saline, FO based-lipid emulsion (FLE), or SO based-lipid emulsion (SLE) iv. The oils were provided at 0.2 g/kg/day for three consecutive days. The animals were sacrificed 24 h after the last administration, blood was collected for plasma, WBC and RBC separation and tissues removed. Fatty acids were analysed by gas chromatography.

RESULTS

FO resulted in higher eicosapentaenoic acid (EPA) in plasma PC and liver than the control. FLE resulted in higher EPA, docosahexaenoic acid (DHA) and total n-3 PUFA in plasma PC, WBC and liver than both the control and SLE groups. EPA, DHA and total n-3 PUFA were higher in the heart with FLE compared with SLE. Individual and total n-3 PUFA were higher in plasma PC, WBC, liver and heart with FLE than with FO given by gavage.

CONCLUSION

Short-term iv administration of n-3 PUFA appears to be more effective at increasing EPA and DHA status in plasma, WBC, liver and heart than oral administration. This might be important for rapid treatment with n-3 PUFA.

Authors+Show Affiliations

Departamento de Fisiologia, Universidade Federal de São Paulo, São Paulo, Brazil; Human Development and Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom. Electronic address: Karina.barros@unifesp.br.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23541913

Citation

Barros, Karina V., et al. "Fatty Acids in Plasma, White and Red Blood Cells, and Tissues After Oral or Intravenous Administration of Fish Oil in Rats." Clinical Nutrition (Edinburgh, Scotland), vol. 32, no. 6, 2013, pp. 993-8.
Barros KV, Carvalho PO, Cassulino AP, et al. Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats. Clin Nutr. 2013;32(6):993-8.
Barros, K. V., Carvalho, P. O., Cassulino, A. P., Andrade, I., West, A. L., Miles, E. A., Calder, P. C., & Silveira, V. L. (2013). Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats. Clinical Nutrition (Edinburgh, Scotland), 32(6), 993-8. https://doi.org/10.1016/j.clnu.2013.02.010
Barros KV, et al. Fatty Acids in Plasma, White and Red Blood Cells, and Tissues After Oral or Intravenous Administration of Fish Oil in Rats. Clin Nutr. 2013;32(6):993-8. PubMed PMID: 23541913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fatty acids in plasma, white and red blood cells, and tissues after oral or intravenous administration of fish oil in rats. AU - Barros,Karina V, AU - Carvalho,Patricia O, AU - Cassulino,Ana P, AU - Andrade,Iracema, AU - West,Annette L, AU - Miles,Elizabeth A, AU - Calder,Philip C, AU - Silveira,Vera L F, Y1 - 2013/03/05/ PY - 2012/11/27/received PY - 2013/02/08/revised PY - 2013/02/21/accepted PY - 2013/4/2/entrez PY - 2013/4/2/pubmed PY - 2014/8/19/medline KW - ARA KW - DHA KW - DPA KW - EPA KW - FA KW - FLE KW - FO KW - Lipid emulsion KW - PUFA KW - Polyunsaturated fatty acids KW - RBC KW - SLE KW - SO KW - WBC KW - arachidonic fatty acid KW - docosahexaenoic acid KW - docosapentaenoic acid KW - eicosapentaenoic acid KW - fatty acid KW - fish oil KW - fish oil based lipid emulsion KW - n-3 PUFA KW - n-6 PUFA KW - polyunsaturated fatty acid KW - red blood cell KW - soybean oil KW - soybean oil based lipid emulsion KW - white blood cell SP - 993 EP - 8 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 32 IS - 6 N2 - BACKGROUND & AIMS: The importance of route of administration of omega-3 (n-3) polyunsaturated fatty acids (PUFA) (oral vs intravenous (iv)) is not clear. We determined the relative concentrations of fatty acids in plasma phosphatidylcholine (PC), red blood cells (RBC), white blood cells (WBC) and several tissues after short-term oral or iv administration of soybean oil (SO) or fish oil (FO). METHODS: Wistar rats (n = 6/group) received saline, FO, or SO by gavage or saline, FO based-lipid emulsion (FLE), or SO based-lipid emulsion (SLE) iv. The oils were provided at 0.2 g/kg/day for three consecutive days. The animals were sacrificed 24 h after the last administration, blood was collected for plasma, WBC and RBC separation and tissues removed. Fatty acids were analysed by gas chromatography. RESULTS: FO resulted in higher eicosapentaenoic acid (EPA) in plasma PC and liver than the control. FLE resulted in higher EPA, docosahexaenoic acid (DHA) and total n-3 PUFA in plasma PC, WBC and liver than both the control and SLE groups. EPA, DHA and total n-3 PUFA were higher in the heart with FLE compared with SLE. Individual and total n-3 PUFA were higher in plasma PC, WBC, liver and heart with FLE than with FO given by gavage. CONCLUSION: Short-term iv administration of n-3 PUFA appears to be more effective at increasing EPA and DHA status in plasma, WBC, liver and heart than oral administration. This might be important for rapid treatment with n-3 PUFA. SN - 1532-1983 UR - https://www.unboundmedicine.com/medline/citation/23541913/Fatty_acids_in_plasma_white_and_red_blood_cells_and_tissues_after_oral_or_intravenous_administration_of_fish_oil_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261-5614(13)00074-5 DB - PRIME DP - Unbound Medicine ER -