TY - JOUR T1 - Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars. AU - Hughes,Stephen R, AU - Bang,Sookie S, AU - Cox,Elby J, AU - Schoepke,Andrew, AU - Ochwat,Kate, AU - Pinkelman,Rebecca, AU - Nelson,Danielle, AU - Qureshi,Nasib, AU - Gibbons,William R, AU - Kurtzman,Cletus P, AU - Bischoff,Kenneth M, AU - Liu,Siqing, AU - Cote,Gregory L, AU - Rich,Joseph O, AU - Jones,Marjorie A, AU - Cedeño,David, AU - Doran-Peterson,Joy, AU - Riaño-Herrera,Nestor M, AU - Rodríguez-Valencia,Nelson, AU - López-Núñez,Juan C, Y1 - 2013/03/29/ PY - 2013/4/2/entrez PY - 2013/4/2/pubmed PY - 2013/12/16/medline KW - Kluyveromyces marxianus yeast KW - biomass sugars KW - thermotolerant ethanologenic yeast KW - yeast UV-C mutagenesis SP - 276 EP - 90 JF - Journal of laboratory automation JO - J Lab Autom VL - 18 IS - 4 N2 - The yeast Kluyveromyces marxianus is a potential microbial catalyst for fuel ethanol production from a wide range of biomass substrates. To improve its growth and ethanol yield at elevated temperature under microaerophilic conditions, K. marxianus NRRL Y-1109 was irradiated with UV-C using automated protocols on a robotic platform for picking and spreading irradiated cultures and for processing the resulting plates. The plates were incubated under anaerobic conditions on xylose or glucose for 5 mo at 46 °C. Two K. marxianus mutant strains (designated 7-1 and 8-1) survived and were isolated from the glucose plates. Both mutant strains, but not wild type, grew aerobically on glucose at 47 °C. All strains grew anaerobically at 46 °C on glucose, galactose, galacturonic acid, and pectin; however, only 7-1 grew anaerobically on xylose at 46 °C. Saccharomyces cerevisiae NRRL Y-2403 did not grow at 46 °C on any of these substrates. With glucose as a carbon source, ethanol yield after 3 d at 46 °C was higher for 8-1 than for wild type (0.51 and 0.43 g ethanol/g glucose, respectively). With galacturonic acid as a carbon source, the ethanol yield after 7 d at 46 °C was higher for 7-1 than for wild type (0.48 and 0.34 g ethanol/g galacturonic acid, respectively). These mutant strains have potential application in fuel ethanol production at elevated temperature from sugar constituents of starch, sucrose, pectin, and cellulosic biomass. SN - 2211-0690 UR - https://www.unboundmedicine.com/medline/citation/23543482/Automated_UV_C_mutagenesis_of_Kluyveromyces_marxianus_NRRL_Y_1109_and_selection_for_microaerophilic_growth_and_ethanol_production_at_elevated_temperature_on_biomass_sugars_ L2 - https://journals.sagepub.com/doi/10.1177/2211068213480037?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -