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Plasmatic higher levels of homocysteine in non-alcoholic fatty liver disease (NAFLD).
Nutr J. 2013 Apr 02; 12:37.NJ

Abstract

BACKGROUND

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease, which includes a spectrum of hepatic pathology such as simple steatosis, steatohepatitis, fibrosis and cirrhosis. The increased serum levels of homocysteine (Hcy) may be associated with hepatic fat accumulation. Genetic mutations in the folate route may only mildly impair Hcy metabolism. The aim of this study was to investigate the relation between liver steatosis with plasma homocysteine level and MTHFR C677T and A1298C polymorphisms in Brazilian patients with NAFLD.

METHODS

Thirty-five patients diagnosed with NAFLD by liver biopsy and forty-five healthy controls neither age nor sex matched were genotyped for C677T and A1298C MTHFR polymorphisms using PCR-RFLP and PCR-ASA, respectively, and Hcy was determined by HPLC. All patients were negative for markers of Wilson's, hemochromatosis and autoimmune diseases. Their daily alcohol intake was less than 100 g/week. A set of metabolic and serum lipid markers were also measured at the time of liver biopsies.

RESULTS

The plasma Hcy level was higher in NAFLD patients compared to the control group (p = 0.0341). No statistical difference for genotypes 677C/T (p = 0.110) and 1298A/C (p = 0.343) in patients with NAFLD and control subjects was observed. The genotypes distribution was in Hardy-Weinberg equilibrium (677C/T p = 0.694 and 1298 A/C p = 0.188). The group of patients and controls showed a statistically significant difference (p < 0.001) for BMI and HOMA_IR, similarly to HDL cholesterol levels (p < 0,006), AST, ALT, γGT, AP and triglycerides levels (p < 0.001). A negative correlation was observed between levels of vitamin B12 and Hcy concentration (p = 0.005).

CONCLUSION

Our results indicate that plasma Hcy was higher in NAFLD than controls. The MTHFR C677T and A1298C polymorphisms did not differ significantly between groups, despite the 677TT homozygous frequency was higher in patients (17.14%) than in controls (677TT = 4.44%) (p > 0.05). The suggested genetic susceptibility to the MTHFR C677T and A1298C should be confirmed in large population based studies.

Authors+Show Affiliations

School of Medicine, University of Pernambuco, Pernambuco, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23547829

Citation

de Carvalho, Sylene Coutinho Rampche, et al. "Plasmatic Higher Levels of Homocysteine in Non-alcoholic Fatty Liver Disease (NAFLD)." Nutrition Journal, vol. 12, 2013, p. 37.
de Carvalho SC, Muniz MT, Siqueira MD, et al. Plasmatic higher levels of homocysteine in non-alcoholic fatty liver disease (NAFLD). Nutr J. 2013;12:37.
de Carvalho, S. C., Muniz, M. T., Siqueira, M. D., Siqueira, E. R., Gomes, A. V., Silva, K. A., Bezerra, L. C., D'Almeida, V., de Oliveira, C. P., & Pereira, L. M. (2013). Plasmatic higher levels of homocysteine in non-alcoholic fatty liver disease (NAFLD). Nutrition Journal, 12, 37. https://doi.org/10.1186/1475-2891-12-37
de Carvalho SC, et al. Plasmatic Higher Levels of Homocysteine in Non-alcoholic Fatty Liver Disease (NAFLD). Nutr J. 2013 Apr 2;12:37. PubMed PMID: 23547829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmatic higher levels of homocysteine in non-alcoholic fatty liver disease (NAFLD). AU - de Carvalho,Sylene Coutinho Rampche, AU - Muniz,Maria Tereza Cartaxo, AU - Siqueira,Maria Deozete Vieira, AU - Siqueira,Erika Rabelo Forte, AU - Gomes,Adriana Vieira, AU - Silva,Karina Alves, AU - Bezerra,Laís Carvalho Luma, AU - D'Almeida,Vânia, AU - de Oliveira,Claudia Pinto Marques Souza, AU - Pereira,Leila Maria M Beltrão, Y1 - 2013/04/02/ PY - 2012/08/15/received PY - 2013/03/08/accepted PY - 2013/4/4/entrez PY - 2013/4/4/pubmed PY - 2013/7/13/medline SP - 37 EP - 37 JF - Nutrition journal JO - Nutr J VL - 12 N2 - BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease, which includes a spectrum of hepatic pathology such as simple steatosis, steatohepatitis, fibrosis and cirrhosis. The increased serum levels of homocysteine (Hcy) may be associated with hepatic fat accumulation. Genetic mutations in the folate route may only mildly impair Hcy metabolism. The aim of this study was to investigate the relation between liver steatosis with plasma homocysteine level and MTHFR C677T and A1298C polymorphisms in Brazilian patients with NAFLD. METHODS: Thirty-five patients diagnosed with NAFLD by liver biopsy and forty-five healthy controls neither age nor sex matched were genotyped for C677T and A1298C MTHFR polymorphisms using PCR-RFLP and PCR-ASA, respectively, and Hcy was determined by HPLC. All patients were negative for markers of Wilson's, hemochromatosis and autoimmune diseases. Their daily alcohol intake was less than 100 g/week. A set of metabolic and serum lipid markers were also measured at the time of liver biopsies. RESULTS: The plasma Hcy level was higher in NAFLD patients compared to the control group (p = 0.0341). No statistical difference for genotypes 677C/T (p = 0.110) and 1298A/C (p = 0.343) in patients with NAFLD and control subjects was observed. The genotypes distribution was in Hardy-Weinberg equilibrium (677C/T p = 0.694 and 1298 A/C p = 0.188). The group of patients and controls showed a statistically significant difference (p < 0.001) for BMI and HOMA_IR, similarly to HDL cholesterol levels (p < 0,006), AST, ALT, γGT, AP and triglycerides levels (p < 0.001). A negative correlation was observed between levels of vitamin B12 and Hcy concentration (p = 0.005). CONCLUSION: Our results indicate that plasma Hcy was higher in NAFLD than controls. The MTHFR C677T and A1298C polymorphisms did not differ significantly between groups, despite the 677TT homozygous frequency was higher in patients (17.14%) than in controls (677TT = 4.44%) (p > 0.05). The suggested genetic susceptibility to the MTHFR C677T and A1298C should be confirmed in large population based studies. SN - 1475-2891 UR - https://www.unboundmedicine.com/medline/citation/23547829/Plasmatic_higher_levels_of_homocysteine_in_non_alcoholic_fatty_liver_disease__NAFLD__ L2 - https://nutritionj.biomedcentral.com/articles/10.1186/1475-2891-12-37 DB - PRIME DP - Unbound Medicine ER -