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Aqueous extract of Artemisia capillaris exerts hepatoprotective action in alcohol-pyrazole-fed rat model.
J Ethnopharmacol 2013; 147(3):662-70JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Artemisia capillaris, also called "InJin" in Korean, has been widely used to treat various hepatic disorders in traditional Oriental medicine.

AIMS

The purpose of this study is to evaluate the hepatoprotective effect of Artemisia capillaris (aqueous extract, WAC) on alcoholic liver injury.

MATERIALS AND METHODS

Liver injury was induced by oral administration of 30% alcohol (10 mL/kg, twice per day) plus pyrazole (PRZ, 30 mg/kg) with/without WAC (50, 100mg/kg, orally once per day) or silymarin (50mg/kg) for 10 days. The hepatoprotective effects were assessed by observing histopathological changes, hepatic transaminase enzymes, hepatic oxidation and antioxidant parameters, inflammatory cytokines, and alcohol metabolic enzymes in serum and hepatic gene expression level, respectively.

RESULTS

Alcohol-PRZ treatment drastically increased the serum levels of aspartate transaminase (AST), alanine transaminase (ALT), and malondialdehyde (MDA) levels in serum and liver tissues while these changes were significantly ameliorated by WAC administration (p<0.05 or 0.01). The prominent microvesicular steatosis and mild necrosis in hepatic histopathology were induced by alcohol-PRZ treatment, but notably attenuated by WAC administration. Moreover, the alcohol-PRZ treatment-induced depletions of the antioxidant components including glutathione content, total antioxidant capacity (TAC), activities of glutathione peroxidase (GSH-Px), reductase (GSH-Rd), catalase, and superoxide dismutase (SOD) were significantly ameliorated by WAC administration (p<0.05, except GSH-Rd). These results were in accordance with the modulation of NF-E2-related factor (Nrf2) and heme oxygenase-1 (HO-1) gene expression. Alcohol-PRZ treatment increased the levels of tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β) in hepatic tissues. However they were significantly normalized by WAC administration (p<0.05 or 0.01). In addition, WAC administration significantly attenuated the alterations of aldehyde dehydrogenase (ALDH) level in serum and hepatic gene expressions of ALDH and alcohol dehydrogenase (ADH).

CONCLUSIONS

These results support the relevance in clinical use of Artemisia capillaris for alcohol-associated hepatic disorders. The underlying mechanisms may involve both enhancement of antioxidant activities and modulation of proinflammatory cytokines.

Authors+Show Affiliations

Liver and Immunology Research Center, Institute of Traditional Medicine and Bioscience of Daejeon University, 22-5 Daeheung-dong, Jung-gu, Daejeon 301-724, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23548584

Citation

Choi, Min-Kyung, et al. "Aqueous Extract of Artemisia Capillaris Exerts Hepatoprotective Action in Alcohol-pyrazole-fed Rat Model." Journal of Ethnopharmacology, vol. 147, no. 3, 2013, pp. 662-70.
Choi MK, Han JM, Kim HG, et al. Aqueous extract of Artemisia capillaris exerts hepatoprotective action in alcohol-pyrazole-fed rat model. J Ethnopharmacol. 2013;147(3):662-70.
Choi, M. K., Han, J. M., Kim, H. G., Lee, J. S., Lee, J. S., Wang, J. H., ... Son, C. G. (2013). Aqueous extract of Artemisia capillaris exerts hepatoprotective action in alcohol-pyrazole-fed rat model. Journal of Ethnopharmacology, 147(3), pp. 662-70. doi:10.1016/j.jep.2013.03.065.
Choi MK, et al. Aqueous Extract of Artemisia Capillaris Exerts Hepatoprotective Action in Alcohol-pyrazole-fed Rat Model. J Ethnopharmacol. 2013 Jun 3;147(3):662-70. PubMed PMID: 23548584.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aqueous extract of Artemisia capillaris exerts hepatoprotective action in alcohol-pyrazole-fed rat model. AU - Choi,Min-Kyung, AU - Han,Jong-Min, AU - Kim,Hyeong-Geug, AU - Lee,Jin-Suk, AU - Lee,Jong-Suk, AU - Wang,Jing-Hua, AU - Son,Seung-Wan, AU - Park,Hye-Jung, AU - Son,Chang-Gue, Y1 - 2013/03/30/ PY - 2012/12/28/received PY - 2013/03/21/revised PY - 2013/03/23/accepted PY - 2013/4/4/entrez PY - 2013/4/4/pubmed PY - 2013/10/23/medline SP - 662 EP - 70 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 147 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Artemisia capillaris, also called "InJin" in Korean, has been widely used to treat various hepatic disorders in traditional Oriental medicine. AIMS: The purpose of this study is to evaluate the hepatoprotective effect of Artemisia capillaris (aqueous extract, WAC) on alcoholic liver injury. MATERIALS AND METHODS: Liver injury was induced by oral administration of 30% alcohol (10 mL/kg, twice per day) plus pyrazole (PRZ, 30 mg/kg) with/without WAC (50, 100mg/kg, orally once per day) or silymarin (50mg/kg) for 10 days. The hepatoprotective effects were assessed by observing histopathological changes, hepatic transaminase enzymes, hepatic oxidation and antioxidant parameters, inflammatory cytokines, and alcohol metabolic enzymes in serum and hepatic gene expression level, respectively. RESULTS: Alcohol-PRZ treatment drastically increased the serum levels of aspartate transaminase (AST), alanine transaminase (ALT), and malondialdehyde (MDA) levels in serum and liver tissues while these changes were significantly ameliorated by WAC administration (p<0.05 or 0.01). The prominent microvesicular steatosis and mild necrosis in hepatic histopathology were induced by alcohol-PRZ treatment, but notably attenuated by WAC administration. Moreover, the alcohol-PRZ treatment-induced depletions of the antioxidant components including glutathione content, total antioxidant capacity (TAC), activities of glutathione peroxidase (GSH-Px), reductase (GSH-Rd), catalase, and superoxide dismutase (SOD) were significantly ameliorated by WAC administration (p<0.05, except GSH-Rd). These results were in accordance with the modulation of NF-E2-related factor (Nrf2) and heme oxygenase-1 (HO-1) gene expression. Alcohol-PRZ treatment increased the levels of tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β) in hepatic tissues. However they were significantly normalized by WAC administration (p<0.05 or 0.01). In addition, WAC administration significantly attenuated the alterations of aldehyde dehydrogenase (ALDH) level in serum and hepatic gene expressions of ALDH and alcohol dehydrogenase (ADH). CONCLUSIONS: These results support the relevance in clinical use of Artemisia capillaris for alcohol-associated hepatic disorders. The underlying mechanisms may involve both enhancement of antioxidant activities and modulation of proinflammatory cytokines. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23548584/Aqueous_extract_of_Artemisia_capillaris_exerts_hepatoprotective_action_in_alcohol_pyrazole_fed_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00221-3 DB - PRIME DP - Unbound Medicine ER -