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The inhibitor of wax 1 locus (Iw1) prevents formation of β- and OH-β-diketones in wheat cuticular waxes and maps to a sub-cM interval on chromosome arm 2BS.
Plant J. 2013 Jun; 74(6):989-1002.PJ

Abstract

Glaucousness is described as the scattering effect of visible light from wax deposited on the cuticle of plant aerial organs. In wheat, two dominant genes lead to non-glaucous phenotypes: Inhibitor of wax 1 (Iw1) and Iw2. The molecular mechanisms and the exact extent (beyond visual assessment) by which these genes affect the composition and quantity of cuticular wax is unclear. To describe the Iw1 locus we used a genetic approach with detailed biochemical characterization of wax compounds. Using synteny and a large number of F2 gametes, Iw1 was fine-mapped to a sub-cM genetic interval on wheat chromosome arm 2BS, which includes a single collinear gene from the corresponding Brachypodium and rice physical maps. The major components of flag leaf and peduncle cuticular waxes included primary alcohols, β-diketones and n-alkanes. Small amounts of C19-C27 alkyl and methylalkylresorcinols that have not previously been described in wheat waxes were identified. Using six pairs of BC2 F3 near-isogenic lines, we show that Iw1 inhibits the formation of β- and hydroxy-β-diketones in the peduncle and flag leaf blade cuticles. This inhibitory effect is independent of genetic background or tissue, and is accompanied by minor but consistent increases in n-alkanes and C24 primary alcohols. No differences were found in cuticle thickness and carbon isotope discrimination in near-isogenic lines differing at Iw1.

Authors+Show Affiliations

John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23551421

Citation

Adamski, Nikolai M., et al. "The Inhibitor of Wax 1 Locus (Iw1) Prevents Formation of Β- and OH-β-diketones in Wheat Cuticular Waxes and Maps to a sub-cM Interval On Chromosome Arm 2BS." The Plant Journal : for Cell and Molecular Biology, vol. 74, no. 6, 2013, pp. 989-1002.
Adamski NM, Bush MS, Simmonds J, et al. The inhibitor of wax 1 locus (Iw1) prevents formation of β- and OH-β-diketones in wheat cuticular waxes and maps to a sub-cM interval on chromosome arm 2BS. Plant J. 2013;74(6):989-1002.
Adamski, N. M., Bush, M. S., Simmonds, J., Turner, A. S., Mugford, S. G., Jones, A., Findlay, K., Pedentchouk, N., von Wettstein-Knowles, P., & Uauy, C. (2013). The inhibitor of wax 1 locus (Iw1) prevents formation of β- and OH-β-diketones in wheat cuticular waxes and maps to a sub-cM interval on chromosome arm 2BS. The Plant Journal : for Cell and Molecular Biology, 74(6), 989-1002. https://doi.org/10.1111/tpj.12185
Adamski NM, et al. The Inhibitor of Wax 1 Locus (Iw1) Prevents Formation of Β- and OH-β-diketones in Wheat Cuticular Waxes and Maps to a sub-cM Interval On Chromosome Arm 2BS. Plant J. 2013;74(6):989-1002. PubMed PMID: 23551421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The inhibitor of wax 1 locus (Iw1) prevents formation of β- and OH-β-diketones in wheat cuticular waxes and maps to a sub-cM interval on chromosome arm 2BS. AU - Adamski,Nikolai M, AU - Bush,Maxwell S, AU - Simmonds,James, AU - Turner,Adrian S, AU - Mugford,Sarah G, AU - Jones,Alan, AU - Findlay,Kim, AU - Pedentchouk,Nikolai, AU - von Wettstein-Knowles,Penny, AU - Uauy,Cristobal, Y1 - 2013/04/25/ PY - 2013/02/04/received PY - 2013/03/21/revised PY - 2013/03/25/accepted PY - 2013/4/5/entrez PY - 2013/4/5/pubmed PY - 2014/1/11/medline SP - 989 EP - 1002 JF - The Plant journal : for cell and molecular biology JO - Plant J VL - 74 IS - 6 N2 - Glaucousness is described as the scattering effect of visible light from wax deposited on the cuticle of plant aerial organs. In wheat, two dominant genes lead to non-glaucous phenotypes: Inhibitor of wax 1 (Iw1) and Iw2. The molecular mechanisms and the exact extent (beyond visual assessment) by which these genes affect the composition and quantity of cuticular wax is unclear. To describe the Iw1 locus we used a genetic approach with detailed biochemical characterization of wax compounds. Using synteny and a large number of F2 gametes, Iw1 was fine-mapped to a sub-cM genetic interval on wheat chromosome arm 2BS, which includes a single collinear gene from the corresponding Brachypodium and rice physical maps. The major components of flag leaf and peduncle cuticular waxes included primary alcohols, β-diketones and n-alkanes. Small amounts of C19-C27 alkyl and methylalkylresorcinols that have not previously been described in wheat waxes were identified. Using six pairs of BC2 F3 near-isogenic lines, we show that Iw1 inhibits the formation of β- and hydroxy-β-diketones in the peduncle and flag leaf blade cuticles. This inhibitory effect is independent of genetic background or tissue, and is accompanied by minor but consistent increases in n-alkanes and C24 primary alcohols. No differences were found in cuticle thickness and carbon isotope discrimination in near-isogenic lines differing at Iw1. SN - 1365-313X UR - https://www.unboundmedicine.com/medline/citation/23551421/The_inhibitor_of_wax_1_locus__Iw1__prevents_formation_of_β__and_OH_β_diketones_in_wheat_cuticular_waxes_and_maps_to_a_sub_cM_interval_on_chromosome_arm_2BS_ L2 - https://doi.org/10.1111/tpj.12185 DB - PRIME DP - Unbound Medicine ER -