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TGF-β superfamily gene expression and induction of the Runx1 transcription factor in adult neurogenic regions after brain injury.
PLoS One. 2013; 8(3):e59250.Plos

Abstract

Traumatic brain injury (TBI) increases neurogenesis in the forebrain subventricular zone (SVZ) and the hippocampal dentate gyrus (DG). Transforming growth factor-β (TGF-β) superfamily cytokines are important regulators of adult neurogenesis, but their involvement in the regulation of this process after brain injury is unclear. We subjected adult mice to controlled cortical impact (CCI) injury, and isolated RNA from the SVZ and DG at different post-injury time points. qPCR array analysis showed that cortical injury caused significant alterations in the mRNA expression of components and targets of the TGF-β, BMP, and activin signaling pathways in the SVZ and DG after injury, suggesting that these pathways could regulate post-injury neurogenesis. In both neurogenic regions, the injury also induced expression of Runt-related transcription factor-1 (Runx1), which can interact with intracellular TGF-β Smad signaling pathways. CCI injury strongly induced Runx1 expression in activated and proliferating microglial cells throughout the neurogenic regions. Runx1 protein was also expressed in a subset of Nestin- and GFAP-expressing putative neural stem or progenitor cells in the DG and SVZ after injury. In the DG only, these Runx1+ progenitors proliferated. Our data suggest potential roles for Runx1 in the processes of microglial cell activation and proliferation and in neural stem cell proliferation after TBI.

Authors+Show Affiliations

Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23555640

Citation

Logan, Trevor T., et al. "TGF-β Superfamily Gene Expression and Induction of the Runx1 Transcription Factor in Adult Neurogenic Regions After Brain Injury." PloS One, vol. 8, no. 3, 2013, pp. e59250.
Logan TT, Villapol S, Symes AJ. TGF-β superfamily gene expression and induction of the Runx1 transcription factor in adult neurogenic regions after brain injury. PLoS One. 2013;8(3):e59250.
Logan, T. T., Villapol, S., & Symes, A. J. (2013). TGF-β superfamily gene expression and induction of the Runx1 transcription factor in adult neurogenic regions after brain injury. PloS One, 8(3), e59250. https://doi.org/10.1371/journal.pone.0059250
Logan TT, Villapol S, Symes AJ. TGF-β Superfamily Gene Expression and Induction of the Runx1 Transcription Factor in Adult Neurogenic Regions After Brain Injury. PLoS One. 2013;8(3):e59250. PubMed PMID: 23555640.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TGF-β superfamily gene expression and induction of the Runx1 transcription factor in adult neurogenic regions after brain injury. AU - Logan,Trevor T, AU - Villapol,Sonia, AU - Symes,Aviva J, Y1 - 2013/03/21/ PY - 2012/09/05/received PY - 2013/02/13/accepted PY - 2013/4/5/entrez PY - 2013/4/5/pubmed PY - 2013/9/26/medline SP - e59250 EP - e59250 JF - PloS one JO - PLoS One VL - 8 IS - 3 N2 - Traumatic brain injury (TBI) increases neurogenesis in the forebrain subventricular zone (SVZ) and the hippocampal dentate gyrus (DG). Transforming growth factor-β (TGF-β) superfamily cytokines are important regulators of adult neurogenesis, but their involvement in the regulation of this process after brain injury is unclear. We subjected adult mice to controlled cortical impact (CCI) injury, and isolated RNA from the SVZ and DG at different post-injury time points. qPCR array analysis showed that cortical injury caused significant alterations in the mRNA expression of components and targets of the TGF-β, BMP, and activin signaling pathways in the SVZ and DG after injury, suggesting that these pathways could regulate post-injury neurogenesis. In both neurogenic regions, the injury also induced expression of Runt-related transcription factor-1 (Runx1), which can interact with intracellular TGF-β Smad signaling pathways. CCI injury strongly induced Runx1 expression in activated and proliferating microglial cells throughout the neurogenic regions. Runx1 protein was also expressed in a subset of Nestin- and GFAP-expressing putative neural stem or progenitor cells in the DG and SVZ after injury. In the DG only, these Runx1+ progenitors proliferated. Our data suggest potential roles for Runx1 in the processes of microglial cell activation and proliferation and in neural stem cell proliferation after TBI. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23555640/TGF_β_superfamily_gene_expression_and_induction_of_the_Runx1_transcription_factor_in_adult_neurogenic_regions_after_brain_injury_ L2 - https://dx.plos.org/10.1371/journal.pone.0059250 DB - PRIME DP - Unbound Medicine ER -