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Functional analysis and expressional characterization of rice ankyrin repeat-containing protein, OsPIANK1, in basal defense against Magnaporthe oryzae attack.
PLoS One 2013; 8(3):e59699Plos

Abstract

The ankyrin repeat-containing protein gene OsPIANK1 (AK068021) in rice (Oryza sativa L.) was previously shown to be upregulated following infection with the rice leaf blight pathogen Xanthomonas oryzae pv oryzae (Xoo). In this study, we further characterized the role of OsPIANK1 in basal defense against Magnaporthe oryzae (M.oryzae) by 5' deletion analysis of its promoter and overexpression of the gene. The promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA). A TCA-element present in the region between -563 bp and -249 bp may be responsible for the OsPIANK1 response to both M.oryzae infection and exogenous SA application. The JERE box, CGTCA-box, and two MYB binding sites locating in the region between -1985 bp and -907 bp may be responsible for the response of OsPIANK1 to exogenous MeJA. OsPIANK1 expression was upregulated after inoculation with M.oryzae and after treatment with exogenous SA and MeJA. Overexpression of OsPIANK1 enhanced resistance of rice to M.oryzae, although it did not confer complete resistance. The enhanced resistance to M.oryzae was accompanied by enhanced transcriptional expression of SA- and JA-dependent genes such as NH1, WKRY13, PAL, AOS2, PR1b, and PR5. This evidence suggests that OsPIANK1 acted as a positive regulator in rice basal defense mediated by SA- and JA-signaling pathways.

Authors+Show Affiliations

College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23555750

Citation

Mou, Shaoliang, et al. "Functional Analysis and Expressional Characterization of Rice Ankyrin Repeat-containing Protein, OsPIANK1, in Basal Defense Against Magnaporthe Oryzae Attack." PloS One, vol. 8, no. 3, 2013, pp. e59699.
Mou S, Liu Z, Guan D, et al. Functional analysis and expressional characterization of rice ankyrin repeat-containing protein, OsPIANK1, in basal defense against Magnaporthe oryzae attack. PLoS ONE. 2013;8(3):e59699.
Mou, S., Liu, Z., Guan, D., Qiu, A., Lai, Y., & He, S. (2013). Functional analysis and expressional characterization of rice ankyrin repeat-containing protein, OsPIANK1, in basal defense against Magnaporthe oryzae attack. PloS One, 8(3), pp. e59699. doi:10.1371/journal.pone.0059699.
Mou S, et al. Functional Analysis and Expressional Characterization of Rice Ankyrin Repeat-containing Protein, OsPIANK1, in Basal Defense Against Magnaporthe Oryzae Attack. PLoS ONE. 2013;8(3):e59699. PubMed PMID: 23555750.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Functional analysis and expressional characterization of rice ankyrin repeat-containing protein, OsPIANK1, in basal defense against Magnaporthe oryzae attack. AU - Mou,Shaoliang, AU - Liu,Zhiqin, AU - Guan,Deyi, AU - Qiu,Ailian, AU - Lai,Yan, AU - He,Shuilin, Y1 - 2013/03/26/ PY - 2012/12/11/received PY - 2013/02/17/accepted PY - 2013/4/5/entrez PY - 2013/4/5/pubmed PY - 2013/10/1/medline SP - e59699 EP - e59699 JF - PloS one JO - PLoS ONE VL - 8 IS - 3 N2 - The ankyrin repeat-containing protein gene OsPIANK1 (AK068021) in rice (Oryza sativa L.) was previously shown to be upregulated following infection with the rice leaf blight pathogen Xanthomonas oryzae pv oryzae (Xoo). In this study, we further characterized the role of OsPIANK1 in basal defense against Magnaporthe oryzae (M.oryzae) by 5' deletion analysis of its promoter and overexpression of the gene. The promoter of OsPIANK1 with 1,985 bps in length was sufficient to induce the OsPIANK1 response to inoculation with M.oryzae and to exogenous application of methyl jasmonate (MeJA) or salicylic acid (SA), but not to exogenous application of abscisic acid (ABA). A TCA-element present in the region between -563 bp and -249 bp may be responsible for the OsPIANK1 response to both M.oryzae infection and exogenous SA application. The JERE box, CGTCA-box, and two MYB binding sites locating in the region between -1985 bp and -907 bp may be responsible for the response of OsPIANK1 to exogenous MeJA. OsPIANK1 expression was upregulated after inoculation with M.oryzae and after treatment with exogenous SA and MeJA. Overexpression of OsPIANK1 enhanced resistance of rice to M.oryzae, although it did not confer complete resistance. The enhanced resistance to M.oryzae was accompanied by enhanced transcriptional expression of SA- and JA-dependent genes such as NH1, WKRY13, PAL, AOS2, PR1b, and PR5. This evidence suggests that OsPIANK1 acted as a positive regulator in rice basal defense mediated by SA- and JA-signaling pathways. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23555750/Functional_analysis_and_expressional_characterization_of_rice_ankyrin_repeat_containing_protein_OsPIANK1_in_basal_defense_against_Magnaporthe_oryzae_attack_ L2 - http://dx.plos.org/10.1371/journal.pone.0059699 DB - PRIME DP - Unbound Medicine ER -