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Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma.
PLoS One 2013; 8(3):e60632Plos

Abstract

BACKGROUND

Diesel exhaust particle (DEP) exposure enhances allergic inflammation and has been linked to the incidence of asthma. Oxidative stress on the thiol molecules cysteine (Cys) and glutathione (GSH) can promote inflammatory host responses. The effect of DEP on the thiol oxidation/reduction (redox) state in the asthmatic lung is unknown.

OBJECTIVE

To determine if DEP exposure alters the Cys or GSH redox state in the asthmatic airway.

METHODS

Bronchoalveolar lavage fluid was obtained from a house dust mite (HDM) induced murine asthma model exposed to DEP. GSH, glutathione disulfide (GSSG), Cys, cystine (CySS), and s-glutathionylated cysteine (CySSG) were determined by high pressure liquid chromatography.

RESULTS

DEP co-administered with HDM, but not DEP or HDM alone, decreased total Cys, increased CySS, and increased CySSG without significantly altering GSH or GSSG.

CONCLUSIONS

DEP exposure promotes oxidation and S-glutathionylation of cysteine amino acids in the asthmatic airway, suggesting a novel mechanism by which DEP may enhance allergic inflammatory responses.

Authors+Show Affiliations

Division of Asthma Research, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, United States of America.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

23555996

Citation

Lee, Gerald B., et al. "Diesel Exhaust Particles Induce Cysteine Oxidation and S-glutathionylation in House Dust Mite Induced Murine Asthma." PloS One, vol. 8, no. 3, 2013, pp. e60632.
Lee GB, Brandt EB, Xiao C, et al. Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma. PLoS ONE. 2013;8(3):e60632.
Lee, G. B., Brandt, E. B., Xiao, C., Gibson, A. M., Le Cras, T. D., Brown, L. A., ... Khurana Hershey, G. K. (2013). Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma. PloS One, 8(3), pp. e60632. doi:10.1371/journal.pone.0060632.
Lee GB, et al. Diesel Exhaust Particles Induce Cysteine Oxidation and S-glutathionylation in House Dust Mite Induced Murine Asthma. PLoS ONE. 2013;8(3):e60632. PubMed PMID: 23555996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diesel exhaust particles induce cysteine oxidation and s-glutathionylation in house dust mite induced murine asthma. AU - Lee,Gerald B, AU - Brandt,Eric B, AU - Xiao,Chang, AU - Gibson,Aaron M, AU - Le Cras,Timothy D, AU - Brown,Lou Ann S, AU - Fitzpatrick,Anne M, AU - Khurana Hershey,Gurjit K, Y1 - 2013/03/29/ PY - 2012/01/19/received PY - 2013/03/01/accepted PY - 2013/4/5/entrez PY - 2013/4/5/pubmed PY - 2013/9/26/medline SP - e60632 EP - e60632 JF - PloS one JO - PLoS ONE VL - 8 IS - 3 N2 - BACKGROUND: Diesel exhaust particle (DEP) exposure enhances allergic inflammation and has been linked to the incidence of asthma. Oxidative stress on the thiol molecules cysteine (Cys) and glutathione (GSH) can promote inflammatory host responses. The effect of DEP on the thiol oxidation/reduction (redox) state in the asthmatic lung is unknown. OBJECTIVE: To determine if DEP exposure alters the Cys or GSH redox state in the asthmatic airway. METHODS: Bronchoalveolar lavage fluid was obtained from a house dust mite (HDM) induced murine asthma model exposed to DEP. GSH, glutathione disulfide (GSSG), Cys, cystine (CySS), and s-glutathionylated cysteine (CySSG) were determined by high pressure liquid chromatography. RESULTS: DEP co-administered with HDM, but not DEP or HDM alone, decreased total Cys, increased CySS, and increased CySSG without significantly altering GSH or GSSG. CONCLUSIONS: DEP exposure promotes oxidation and S-glutathionylation of cysteine amino acids in the asthmatic airway, suggesting a novel mechanism by which DEP may enhance allergic inflammatory responses. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23555996/Diesel_exhaust_particles_induce_cysteine_oxidation_and_s_glutathionylation_in_house_dust_mite_induced_murine_asthma_ L2 - http://dx.plos.org/10.1371/journal.pone.0060632 DB - PRIME DP - Unbound Medicine ER -