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Antimicrobial effects of calcium hydroxide, chlorhexidine, and propolis on Enterococcus faecalis and Candida albicans.
J Investig Clin Dent. 2014 Aug; 5(3):194-200.JI

Abstract

AIM

To evaluate the efficacy of calcium hydroxide (Ca[OH]2), 2% chlorhexidine (CHX) gel, and propolis against both Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) using infected dentine models at two different depths (100 and 200 μm) after 14 days of application.

METHODS

A total of 120 roots of extracted single-rooted human teeth were chemo-mechanically prepared and sterilized. Sixty roots were infected with E. faecalis, and the remaining 60 with C. albicans. Each group was divided into four subgroups (n = 15) to apply intracanal medicaments, namely saline solution (negative control), Ca(OH)2 , CHX, and propolis during the 14 days. Dentine shavings were collected and cultivated. Colony-forming units (c.f.u.) were registered. Statistical analysis was done using the Kruskal-Wallis test, followed by Dunn's/Bonferroni multiple comparison test (P < 0.05).

RESULTS

All experimental agents significantly reduced E. faecalis c.f.u. There was no significant difference between CHX and propolis reducing E. faecalis c.f.u. at 100 and 200 μm. Only CHX had a statistically-significant antifungal efficacy in the C. albicans group at the two depths assessed. CHX was the most potent medicament against both E. faecalis and C. albicans, and Ca(OH)2 was the least.

CONCLUSION

Both CHX and propolis were the most effective against E. faecalis, whereas only CHX had the highest antifungal activity on C. albicans in dentine of extracted teeth.

Authors+Show Affiliations

Department of Endodontics, Faculty of Dentistry, Daniel Alcides Carrión National University, Pasco, Peru.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23559565

Citation

Carbajal Mejía, Jeison B.. "Antimicrobial Effects of Calcium Hydroxide, Chlorhexidine, and Propolis On Enterococcus Faecalis and Candida Albicans." Journal of Investigative and Clinical Dentistry, vol. 5, no. 3, 2014, pp. 194-200.
Carbajal Mejía JB. Antimicrobial effects of calcium hydroxide, chlorhexidine, and propolis on Enterococcus faecalis and Candida albicans. J Investig Clin Dent. 2014;5(3):194-200.
Carbajal Mejía, J. B. (2014). Antimicrobial effects of calcium hydroxide, chlorhexidine, and propolis on Enterococcus faecalis and Candida albicans. Journal of Investigative and Clinical Dentistry, 5(3), 194-200. https://doi.org/10.1111/jicd.12041
Carbajal Mejía JB. Antimicrobial Effects of Calcium Hydroxide, Chlorhexidine, and Propolis On Enterococcus Faecalis and Candida Albicans. J Investig Clin Dent. 2014;5(3):194-200. PubMed PMID: 23559565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial effects of calcium hydroxide, chlorhexidine, and propolis on Enterococcus faecalis and Candida albicans. A1 - Carbajal Mejía,Jeison B, Y1 - 2013/04/04/ PY - 2012/05/05/received PY - 2013/01/19/accepted PY - 2013/4/6/entrez PY - 2013/4/6/pubmed PY - 2015/6/9/medline KW - Candida albicans KW - Enterococcus faecalis KW - calcium hydroxide KW - chlorhexidine KW - propolis SP - 194 EP - 200 JF - Journal of investigative and clinical dentistry JO - J Investig Clin Dent VL - 5 IS - 3 N2 - AIM: To evaluate the efficacy of calcium hydroxide (Ca[OH]2), 2% chlorhexidine (CHX) gel, and propolis against both Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans) using infected dentine models at two different depths (100 and 200 μm) after 14 days of application. METHODS: A total of 120 roots of extracted single-rooted human teeth were chemo-mechanically prepared and sterilized. Sixty roots were infected with E. faecalis, and the remaining 60 with C. albicans. Each group was divided into four subgroups (n = 15) to apply intracanal medicaments, namely saline solution (negative control), Ca(OH)2 , CHX, and propolis during the 14 days. Dentine shavings were collected and cultivated. Colony-forming units (c.f.u.) were registered. Statistical analysis was done using the Kruskal-Wallis test, followed by Dunn's/Bonferroni multiple comparison test (P < 0.05). RESULTS: All experimental agents significantly reduced E. faecalis c.f.u. There was no significant difference between CHX and propolis reducing E. faecalis c.f.u. at 100 and 200 μm. Only CHX had a statistically-significant antifungal efficacy in the C. albicans group at the two depths assessed. CHX was the most potent medicament against both E. faecalis and C. albicans, and Ca(OH)2 was the least. CONCLUSION: Both CHX and propolis were the most effective against E. faecalis, whereas only CHX had the highest antifungal activity on C. albicans in dentine of extracted teeth. SN - 2041-1626 UR - https://www.unboundmedicine.com/medline/citation/23559565/Antimicrobial_effects_of_calcium_hydroxide_chlorhexidine_and_propolis_on_Enterococcus_faecalis_and_Candida_albicans_ L2 - https://doi.org/10.1111/jicd.12041 DB - PRIME DP - Unbound Medicine ER -