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In vitro bioavailability of total selenium and selenium species from seafood.
Food Chem. 2013 Aug 15; 139(1-4):872-7.FC

Abstract

In vitro bioavailability of total selenium and selenium species from different raw seafood has been assessed by using a simulated gastric and intestinal digestion/dialysis method. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to assess total selenium contents after a microwave assisted acid digestion, and also to quantify total selenium in the dialyzable and non-dialyzable fractions. Selenium speciation in the dialyzates was assessed by high performance liquid chromatography (HPLC) coupled with ICP-MS detection. Major Se species (selenium methionine and oxidized selenium methionine) from dialyzate were identified and characterized by HPLC coupled to mass spectrometry (HPLC-MS). Selenocystine was detected at low concentrations while Se-(Methyl)selenocysteine and inorganic selenium species (selenite and selenate) were not detected in the dialyzate. Low bioavailability percentages for total selenium (6.69±3.39 and 5.45±2.44% for fish and mollusk samples, respectively) were obtained. Similar bioavailability percentages was achieved for total selenium as a sum of selenium species (selenocystine plus oxidized selenium methionine and selenium methionine, mainly). HPLC-MS data confirmed SeMet oxidation during the in vitro procedure.

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Authors+Show Affiliations

Moreda-Piñeiro J
Department of Analytical Chemistry, Faculty of Sciences, University of A Coruña, Campus da Zapateira, s/n., 15071 A Coruña, Spain. jorge.moreda@udc.es
Moreda-Piñeiro A
No affiliation info available
Romarís-Hortas V
No affiliation info available
Domínguez-González R
No affiliation info available
Alonso-Rodríguez E
No affiliation info available
López-Mahía P
No affiliation info available
Muniategui-Lorenzo S
No affiliation info available
Prada-Rodríguez D
No affiliation info available
Bermejo-Barrera P
No affiliation info available

MeSH

AnimalsChromatography, High Pressure LiquidCystineFishesMass SpectrometryMolluscaOrganoselenium CompoundsSeafoodSeleniumSelenomethionine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23561184

Citation

Moreda-Piñeiro, Jorge, et al. "In Vitro Bioavailability of Total Selenium and Selenium Species From Seafood." Food Chemistry, vol. 139, no. 1-4, 2013, pp. 872-7.
Moreda-Piñeiro J, Moreda-Piñeiro A, Romarís-Hortas V, et al. In vitro bioavailability of total selenium and selenium species from seafood. Food Chem. 2013;139(1-4):872-7.
Moreda-Piñeiro, J., Moreda-Piñeiro, A., Romarís-Hortas, V., Domínguez-González, R., Alonso-Rodríguez, E., López-Mahía, P., Muniategui-Lorenzo, S., Prada-Rodríguez, D., & Bermejo-Barrera, P. (2013). In vitro bioavailability of total selenium and selenium species from seafood. Food Chemistry, 139(1-4), 872-7. https://doi.org/10.1016/j.foodchem.2013.01.116
Moreda-Piñeiro J, et al. In Vitro Bioavailability of Total Selenium and Selenium Species From Seafood. Food Chem. 2013 Aug 15;139(1-4):872-7. PubMed PMID: 23561184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro bioavailability of total selenium and selenium species from seafood. AU - Moreda-Piñeiro,Jorge, AU - Moreda-Piñeiro,Antonio, AU - Romarís-Hortas,Vanessa, AU - Domínguez-González,Raquel, AU - Alonso-Rodríguez,Elia, AU - López-Mahía,Purificación, AU - Muniategui-Lorenzo,Soledad, AU - Prada-Rodríguez,Darío, AU - Bermejo-Barrera,Pilar, Y1 - 2013/02/10/ PY - 2012/10/02/received PY - 2012/12/05/revised PY - 2013/01/27/accepted PY - 2013/4/9/entrez PY - 2013/4/9/pubmed PY - 2013/10/19/medline SP - 872 EP - 7 JF - Food chemistry JO - Food Chem VL - 139 IS - 1-4 N2 - In vitro bioavailability of total selenium and selenium species from different raw seafood has been assessed by using a simulated gastric and intestinal digestion/dialysis method. Inductively coupled plasma-mass spectrometry (ICP-MS) was used to assess total selenium contents after a microwave assisted acid digestion, and also to quantify total selenium in the dialyzable and non-dialyzable fractions. Selenium speciation in the dialyzates was assessed by high performance liquid chromatography (HPLC) coupled with ICP-MS detection. Major Se species (selenium methionine and oxidized selenium methionine) from dialyzate were identified and characterized by HPLC coupled to mass spectrometry (HPLC-MS). Selenocystine was detected at low concentrations while Se-(Methyl)selenocysteine and inorganic selenium species (selenite and selenate) were not detected in the dialyzate. Low bioavailability percentages for total selenium (6.69±3.39 and 5.45±2.44% for fish and mollusk samples, respectively) were obtained. Similar bioavailability percentages was achieved for total selenium as a sum of selenium species (selenocystine plus oxidized selenium methionine and selenium methionine, mainly). HPLC-MS data confirmed SeMet oxidation during the in vitro procedure. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/23561184/In_vitro_bioavailability_of_total_selenium_and_selenium_species_from_seafood_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(13)00152-0 DB - PRIME DP - Unbound Medicine ER -