Tags

Type your tag names separated by a space and hit enter

Antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn.
Asian Pac J Trop Biomed. 2011 Oct; 1(5):409-12.AP

Abstract

OBJECTIVE

To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet (Z. zerumbet) (L.) Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi.

METHODS

The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol (4.0 L) after concentration. The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract (15.0 g), petroleum ether fraction (6.6 g) and chloroform soluble fraction (5.0 g). The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method. Commercially available kanamycin (30 µg/disc) was used as standard disc and blank discs impregnated with the respective solvents were used as negative control.

RESULTS

At a concentration of 400 µg/disc, all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm. Among the tested samples, the crude ethanol extract showed the highest activity against Vibrio parahemolyticus (V. parahemolyticus). The minimum inhibitory concentration (MIC) of the crude ethanol extract and its fractions were within the value of 128-256 µg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V. parahemolyticus (128 µg/mL).

CONCLUSIONS

It can be concluded that, potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z. zerumbet (L).

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23569803

Citation

Kader, Golam, et al. "Antimicrobial Activities of the Rhizome Extract of Zingiber Zerumbet Linn." Asian Pacific Journal of Tropical Biomedicine, vol. 1, no. 5, 2011, pp. 409-12.
Kader G, Nikkon F, Rashid MA, et al. Antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn. Asian Pac J Trop Biomed. 2011;1(5):409-12.
Kader, G., Nikkon, F., Rashid, M. A., & Yeasmin, T. (2011). Antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn. Asian Pacific Journal of Tropical Biomedicine, 1(5), 409-12. https://doi.org/10.1016/S2221-1691(11)60090-7
Kader G, et al. Antimicrobial Activities of the Rhizome Extract of Zingiber Zerumbet Linn. Asian Pac J Trop Biomed. 2011;1(5):409-12. PubMed PMID: 23569803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn. AU - Kader,Golam, AU - Nikkon,Farjana, AU - Rashid,Mohammad Abdur, AU - Yeasmin,Tanzima, PY - 2011/03/19/received PY - 2011/04/08/revised PY - 2011/04/28/accepted PY - 2013/4/10/entrez PY - 2011/10/1/pubmed PY - 2011/10/1/medline KW - Antibacterial KW - Antifungal KW - Antifungal activity KW - Antimicrobial activity KW - Disc diffusion method KW - Minimum inhibitory concentration (MIC) KW - Pathogenic bacteria KW - Phytochemicals KW - Rhizome KW - Soluble fraction KW - Zingiber zerumbet (L.) Smith SP - 409 EP - 12 JF - Asian Pacific journal of tropical biomedicine JO - Asian Pac J Trop Biomed VL - 1 IS - 5 N2 - OBJECTIVE: To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet (Z. zerumbet) (L.) Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi. METHODS: The fresh rhizomes of Zingiber zerumbet were extracted in cold with ethanol (4.0 L) after concentration. The crude ethanol extract was fractionated by petroleum ether and chloroform to form a suspension of ethanol extract (15.0 g), petroleum ether fraction (6.6 g) and chloroform soluble fraction (5.0 g). The crude ethanol extract and its petroleum ether and chloroform fractions were evaluated for antibacterial and antifungal activity against thirteen pathogenic bacteria and three fungi by the disc diffusion method. Commercially available kanamycin (30 µg/disc) was used as standard disc and blank discs impregnated with the respective solvents were used as negative control. RESULTS: At a concentration of 400 µg/disc, all the samples showed mild to moderate antibacterial and antifungal activity and produced the zone of inhibition ranging from 6 mm to 10 mm. Among the tested samples, the crude ethanol extract showed the highest activity against Vibrio parahemolyticus (V. parahemolyticus). The minimum inhibitory concentration (MIC) of the crude ethanol extract and its fractions were within the value of 128-256 µg/mL against two Gram positive and four Gram negative bacteria and all the samples showed the lowest MIC value against V. parahemolyticus (128 µg/mL). CONCLUSIONS: It can be concluded that, potent antibacterial and antifungal phytochemicals are present in ethanol extract of Z. zerumbet (L). SN - 2221-1691 UR - https://www.unboundmedicine.com/medline/citation/23569803/Antimicrobial_activities_of_the_rhizome_extract_of_Zingiber_zerumbet_Linn_ L2 - https://linkinghub.elsevier.com/retrieve/pii/apjtb-01-05-409 DB - PRIME DP - Unbound Medicine ER -