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In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas.
Asian Pac J Trop Biomed. 2012 Oct; 2(10):774-81.AP

Abstract

OBJECTIVE

To investigate and compare the inhibitory properties of free and bound phenolic extracts of clove bud against carbohydrate hydrolyzing enzymes (alpha-amylase & alpha-glucosidase) and Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.

METHODS

The free phenolics were extracted with 80% (v/v) acetone, while bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Then, the interaction of the extracts with alpha-amylase and alpha-glucosidase was subsequently assessed. Thereafter, the total phenolic contents and antioxidant activities of the extracts were determined.

RESULTS

The result revealed that both extracts inhibited alpha-amylase and alpha-glucosidase in a dose-dependent manner. However, the alpha-glucosidase inhibitory activity of the extracts were significantly (P<0.05) higher than their alpha-amylase inhibitory activity. The free phenolics (31.67 mg/g) and flavonoid (17.28 mg/g) contents were significantly (P<0.05) higher than bound phenolic (23.52 mg/g) and flavonoid (13.70 mg/g) contents. Both extracts also exhibited high antioxidant activities as typified by their high reducing power, 1,1 diphenyl-2- picrylhydrazyl (DPPH) and 2, 2-azinobis-3-ethylbenzo-thiazoline-6-sulfonate (ABTS) radical scavenging abilities, as well as inhibition of Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.

CONCLUSIONS

This study provides a biochemical rationale by which clove elicits therapeutic effect on type 2 diabetes.

Authors+Show Affiliations

Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure-340001, Nigeria.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23569846

Citation

Adefegha, Stephen Adeniyi, and Ganiyu Oboh. "In Vitro Inhibition Activity of Polyphenol-rich Extracts From Syzygium Aromaticum (L.) Merr. & Perry (Clove) Buds Against Carbohydrate Hydrolyzing Enzymes Linked to Type 2 Diabetes and Fe(2+)-induced Lipid Peroxidation in Rat Pancreas." Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 10, 2012, pp. 774-81.
Adefegha SA, Oboh G. In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas. Asian Pac J Trop Biomed. 2012;2(10):774-81.
Adefegha, S. A., & Oboh, G. (2012). In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas. Asian Pacific Journal of Tropical Biomedicine, 2(10), 774-81. https://doi.org/10.1016/S2221-1691(12)60228-7
Adefegha SA, Oboh G. In Vitro Inhibition Activity of Polyphenol-rich Extracts From Syzygium Aromaticum (L.) Merr. & Perry (Clove) Buds Against Carbohydrate Hydrolyzing Enzymes Linked to Type 2 Diabetes and Fe(2+)-induced Lipid Peroxidation in Rat Pancreas. Asian Pac J Trop Biomed. 2012;2(10):774-81. PubMed PMID: 23569846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas. AU - Adefegha,Stephen Adeniyi, AU - Oboh,Ganiyu, PY - 2012/01/15/received PY - 2012/01/27/revised PY - 2012/03/22/accepted PY - 2013/4/10/entrez PY - 2013/4/10/pubmed PY - 2013/4/10/medline KW - Alpha-amylase KW - Alpha-glucosidase KW - Antioxidant KW - Fe2+-induced lipid peroxidation Pancrea KW - Phenol SP - 774 EP - 81 JF - Asian Pacific journal of tropical biomedicine JO - Asian Pac J Trop Biomed VL - 2 IS - 10 N2 - OBJECTIVE: To investigate and compare the inhibitory properties of free and bound phenolic extracts of clove bud against carbohydrate hydrolyzing enzymes (alpha-amylase & alpha-glucosidase) and Fe(2+)-induced lipid peroxidation in rat pancreas in vitro. METHODS: The free phenolics were extracted with 80% (v/v) acetone, while bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Then, the interaction of the extracts with alpha-amylase and alpha-glucosidase was subsequently assessed. Thereafter, the total phenolic contents and antioxidant activities of the extracts were determined. RESULTS: The result revealed that both extracts inhibited alpha-amylase and alpha-glucosidase in a dose-dependent manner. However, the alpha-glucosidase inhibitory activity of the extracts were significantly (P<0.05) higher than their alpha-amylase inhibitory activity. The free phenolics (31.67 mg/g) and flavonoid (17.28 mg/g) contents were significantly (P<0.05) higher than bound phenolic (23.52 mg/g) and flavonoid (13.70 mg/g) contents. Both extracts also exhibited high antioxidant activities as typified by their high reducing power, 1,1 diphenyl-2- picrylhydrazyl (DPPH) and 2, 2-azinobis-3-ethylbenzo-thiazoline-6-sulfonate (ABTS) radical scavenging abilities, as well as inhibition of Fe(2+)-induced lipid peroxidation in rat pancreas in vitro. CONCLUSIONS: This study provides a biochemical rationale by which clove elicits therapeutic effect on type 2 diabetes. SN - 2221-1691 UR - https://www.unboundmedicine.com/medline/citation/23569846/In_vitro_inhibition_activity_of_polyphenol_rich_extracts_from_Syzygium_aromaticum__L___Merr__&_Perry__Clove__buds_against_carbohydrate_hydrolyzing_enzymes_linked_to_type_2_diabetes_and_Fe_2+__induced_lipid_peroxidation_in_rat_pancreas_ L2 - https://linkinghub.elsevier.com/retrieve/pii/apjtb-02-10-774 DB - PRIME DP - Unbound Medicine ER -