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Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases.
Asian Pac J Trop Biomed. 2012 Mar; 2(3):189-94.AP

Abstract

OBJECTIVE

To investigate antibacterial potential of Trifolium alexandrinum (T. alexandrinum) Linn. against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical diseases.

METHODS

Non-polar and polar extracts of the leaves of T. alexandrinum i.e., hexane, dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH) and aqueous (AQ) extracts at five different concentrations (1, 2, 5, 10 and 15 mg/mL) were prepared to evaluate their antibacterial value. NCCL standards were strictly followed to perform antimicrobial disc susceptibility test using disc diffusion method.

RESULTS

Polar extracts demonstrated significant antibacterial activity against tested pathogens. EtOAc and MeOH extracts showed maximum antibacterial activity with higher inhibition zone and were found effective against seventeen of the tested pathogens. While AQ plant extract inhibited the growth of sixteen of the test strains. EtOAc and MeOH plant extracts inhibited the growth of all seven gram positive and ten of the gram negative bacterial strains.

CONCLUSIONS

The present study strongly confirms the effectiveness of crude leaves extracts against tested human pathogenic bacterial strains causing several tropical diseases. Since Egyptian clover is used as a fodder plant, it could be helpful in controlling various infectious diseases associated with cattle as well.

Authors+Show Affiliations

Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202002, State of Uttar Pradesh, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23569896

Citation

Khan, Abdul Viqar, et al. "Antibacterial Activity of Leaves Extracts of Trifolium Alexandrinum Linn. Against Pathogenic Bacteria Causing Tropical Diseases." Asian Pacific Journal of Tropical Biomedicine, vol. 2, no. 3, 2012, pp. 189-94.
Khan AV, Ahmed QU, Shukla I, et al. Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pac J Trop Biomed. 2012;2(3):189-94.
Khan, A. V., Ahmed, Q. U., Shukla, I., & Khan, A. A. (2012). Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. Asian Pacific Journal of Tropical Biomedicine, 2(3), 189-94. https://doi.org/10.1016/S2221-1691(12)60040-9
Khan AV, et al. Antibacterial Activity of Leaves Extracts of Trifolium Alexandrinum Linn. Against Pathogenic Bacteria Causing Tropical Diseases. Asian Pac J Trop Biomed. 2012;2(3):189-94. PubMed PMID: 23569896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activity of leaves extracts of Trifolium alexandrinum Linn. against pathogenic bacteria causing tropical diseases. AU - Khan,Abdul Viqar, AU - Ahmed,Qamar Uddin, AU - Shukla,Indu, AU - Khan,Athar Ali, PY - 2011/08/15/received PY - 2011/09/08/revised PY - 2011/10/03/accepted PY - 2013/4/10/entrez PY - 2013/4/10/pubmed PY - 2013/4/10/medline KW - Antibacterial activity KW - Fabaceae KW - Gram-negative bacteria KW - Gram-positive bacteria KW - Infectious disease KW - Pathogenic bacteria KW - Trifolium alexandrinum L. KW - Tropical disease SP - 189 EP - 94 JF - Asian Pacific journal of tropical biomedicine JO - Asian Pac J Trop Biomed VL - 2 IS - 3 N2 - OBJECTIVE: To investigate antibacterial potential of Trifolium alexandrinum (T. alexandrinum) Linn. against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical diseases. METHODS: Non-polar and polar extracts of the leaves of T. alexandrinum i.e., hexane, dichloromethane (DCM), ethyl acetate (EtOAc), methanol (MeOH) and aqueous (AQ) extracts at five different concentrations (1, 2, 5, 10 and 15 mg/mL) were prepared to evaluate their antibacterial value. NCCL standards were strictly followed to perform antimicrobial disc susceptibility test using disc diffusion method. RESULTS: Polar extracts demonstrated significant antibacterial activity against tested pathogens. EtOAc and MeOH extracts showed maximum antibacterial activity with higher inhibition zone and were found effective against seventeen of the tested pathogens. While AQ plant extract inhibited the growth of sixteen of the test strains. EtOAc and MeOH plant extracts inhibited the growth of all seven gram positive and ten of the gram negative bacterial strains. CONCLUSIONS: The present study strongly confirms the effectiveness of crude leaves extracts against tested human pathogenic bacterial strains causing several tropical diseases. Since Egyptian clover is used as a fodder plant, it could be helpful in controlling various infectious diseases associated with cattle as well. SN - 2221-1691 UR - https://www.unboundmedicine.com/medline/citation/23569896/Antibacterial_activity_of_leaves_extracts_of_Trifolium_alexandrinum_Linn__against_pathogenic_bacteria_causing_tropical_diseases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/apjtb-02-03-189 DB - PRIME DP - Unbound Medicine ER -