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In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals.

Abstract

OBJECTIVE

To assess the In vivo antioxidFant and hepatoprotective activity of methanolic extract of Daucus carota (D. carota) seeds in experimental animals.

METHODS

Methanolic extracts of D. carota seeds is used for hepatoprotection assessment. Oxidative stress were induced in rats by thioacetamide 100 mg/kg s.c, in four groups of rats (two test, standard and toxic control). Two test groups received D. carota seeds extract (DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin (25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle. On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) were estimated in blood serum and antioxidant enzyme like superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and lipid peroxidation (LPO) were estimated in liver homogenate.

RESULTS

A significant decrease in SGPT, SGOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group (P < 0.001) and in case of antioxidant enzyme a significant (P < 0.001) increase in SOD, CAT, GRD, GPX and GST was observed in all drug treated groups as compared with thioacetamide group. But in case of LPO a significant (P < 0.001) reduction was observed as compared to toxic control group.

CONCLUSIONS

DCSE has contributed to the reduction of oxidative stress and the protection of liver in experimental rats.

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    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    23569935

    Citation

    TY - JOUR T1 - In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals. AU - Singh,Kamlesh, AU - Singh,Nisha, AU - Chandy,Anish, AU - Manigauha,Ashish, PY - 2011/8/15/received PY - 2011/8/27/revised PY - 2011/10/24/accepted PY - 2013/4/10/entrez PY - 2013/4/10/pubmed PY - 2013/4/10/medline KW - Antioxidant KW - Biochemical parameters KW - Daucus carota seeds extract KW - Hepatoprotection KW - Silymarin KW - Thioacetamide SP - 385 EP - 8 JF - Asian Pacific journal of tropical biomedicine JO - Asian Pac J Trop Biomed VL - 2 IS - 5 N2 - OBJECTIVE: To assess the In vivo antioxidFant and hepatoprotective activity of methanolic extract of Daucus carota (D. carota) seeds in experimental animals. METHODS: Methanolic extracts of D. carota seeds is used for hepatoprotection assessment. Oxidative stress were induced in rats by thioacetamide 100 mg/kg s.c, in four groups of rats (two test, standard and toxic control). Two test groups received D. carota seeds extract (DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin (25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle. On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) were estimated in blood serum and antioxidant enzyme like superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and lipid peroxidation (LPO) were estimated in liver homogenate. RESULTS: A significant decrease in SGPT, SGOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group (P < 0.001) and in case of antioxidant enzyme a significant (P < 0.001) increase in SOD, CAT, GRD, GPX and GST was observed in all drug treated groups as compared with thioacetamide group. But in case of LPO a significant (P < 0.001) reduction was observed as compared to toxic control group. CONCLUSIONS: DCSE has contributed to the reduction of oxidative stress and the protection of liver in experimental rats. SN - 2221-1691 UR - https://www.unboundmedicine.com/medline/citation/23569935/In_vivo_antioxidant_and_hepatoprotective_activity_of_methanolic_extracts_of_Daucus_carota_seeds_in_experimental_animals_ L2 - http://linkinghub.elsevier.com/retrieve/pii/apjtb-02-05-385 ER -