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Detection of dengue virus nonstructural protein 1 (NS1) by using ELISA as a useful laboratory diagnostic method for dengue virus infection of international travelers.
J Travel Med. 2013 May-Jun; 20(3):185-93.JT

Abstract

BACKGROUND

Dengue virus (DENV) nonstructural protein 1 (NS1) has been used as a novel diagnostic marker during the early phase of DENV infection.

METHODS

Presence of NS1 antigen was examined using 336 serum samples obtained from 276 travelers returning to Japan from Asia, Central and South America, Pacific Islands, and Africa with dengue. Assay specificity was evaluated using 148 non-dengue samples.

RESULTS

Positive rates among four DENV serotypes were 68%-89%. NS1 antigen positive rates were at similar levels between primary infection and secondary infection. NS1 antigen positive rates were 88%-96% on days 1-5, 75%-100% on days 6-10, and 36-60% on ≥ day 11. Positive rates using real-time polymerase chain reaction (RT-PCR) were over 70% on days 1-5, but decreased thereafter.

CONCLUSIONS

The results indicate that NS1 antigen positive rates were higher than those of RT-PCR during longer period of early phase in DENV infection. Thus, NS1 antigen ELISA is a useful tool for confirming DENV infection in international travelers, when it is used in combination with anti-DENV IgM ELISA.

Authors+Show Affiliations

Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23577865

Citation

Moi, Meng Ling, et al. "Detection of Dengue Virus Nonstructural Protein 1 (NS1) By Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers." Journal of Travel Medicine, vol. 20, no. 3, 2013, pp. 185-93.
Moi ML, Omatsu T, Tajima S, et al. Detection of dengue virus nonstructural protein 1 (NS1) by using ELISA as a useful laboratory diagnostic method for dengue virus infection of international travelers. J Travel Med. 2013;20(3):185-93.
Moi, M. L., Omatsu, T., Tajima, S., Lim, C. K., Kotaki, A., Ikeda, M., Harada, F., Ito, M., Saijo, M., Kurane, I., & Takasaki, T. (2013). Detection of dengue virus nonstructural protein 1 (NS1) by using ELISA as a useful laboratory diagnostic method for dengue virus infection of international travelers. Journal of Travel Medicine, 20(3), 185-93. https://doi.org/10.1111/jtm.12018
Moi ML, et al. Detection of Dengue Virus Nonstructural Protein 1 (NS1) By Using ELISA as a Useful Laboratory Diagnostic Method for Dengue Virus Infection of International Travelers. J Travel Med. 2013 May-Jun;20(3):185-93. PubMed PMID: 23577865.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of dengue virus nonstructural protein 1 (NS1) by using ELISA as a useful laboratory diagnostic method for dengue virus infection of international travelers. AU - Moi,Meng Ling, AU - Omatsu,Tsutomu, AU - Tajima,Shigeru, AU - Lim,Chang-Kweng, AU - Kotaki,Akira, AU - Ikeda,Makiko, AU - Harada,Fumiue, AU - Ito,Mikako, AU - Saijo,Masayuki, AU - Kurane,Ichiro, AU - Takasaki,Tomohiko, Y1 - 2013/03/08/ PY - 2012/04/13/received PY - 2012/11/30/revised PY - 2012/12/05/accepted PY - 2013/4/13/entrez PY - 2013/4/13/pubmed PY - 2013/11/5/medline SP - 185 EP - 93 JF - Journal of travel medicine JO - J Travel Med VL - 20 IS - 3 N2 - BACKGROUND: Dengue virus (DENV) nonstructural protein 1 (NS1) has been used as a novel diagnostic marker during the early phase of DENV infection. METHODS: Presence of NS1 antigen was examined using 336 serum samples obtained from 276 travelers returning to Japan from Asia, Central and South America, Pacific Islands, and Africa with dengue. Assay specificity was evaluated using 148 non-dengue samples. RESULTS: Positive rates among four DENV serotypes were 68%-89%. NS1 antigen positive rates were at similar levels between primary infection and secondary infection. NS1 antigen positive rates were 88%-96% on days 1-5, 75%-100% on days 6-10, and 36-60% on ≥ day 11. Positive rates using real-time polymerase chain reaction (RT-PCR) were over 70% on days 1-5, but decreased thereafter. CONCLUSIONS: The results indicate that NS1 antigen positive rates were higher than those of RT-PCR during longer period of early phase in DENV infection. Thus, NS1 antigen ELISA is a useful tool for confirming DENV infection in international travelers, when it is used in combination with anti-DENV IgM ELISA. SN - 1708-8305 UR - https://www.unboundmedicine.com/medline/citation/23577865/Detection_of_dengue_virus_nonstructural_protein_1__NS1__by_using_ELISA_as_a_useful_laboratory_diagnostic_method_for_dengue_virus_infection_of_international_travelers_ L2 - https://academic.oup.com/jtm/article-lookup/doi/10.1111/jtm.12018 DB - PRIME DP - Unbound Medicine ER -