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Neuropeptide FF attenuates the acquisition and the expression of conditioned place aversion to endomorphin-2 in mice.
Behav Brain Res. 2013 Jul 01; 248:51-6.BB

Abstract

It has been demonstrated that the endogenous mu opioid (MOP) agonist endomorphin-2 (EM-2) produces conditioned place aversion (CPA) and in contrast, morphine exerts opposite action. Neuropeptide FF (NPFF) was reported to act as a functional antagonist of mu opioid receptor and to exert opioid-modulating activities. The present study examined the influence of NPFF on the rewarding action of EM-2, using the unbiased conditioned place preference (CPP) paradigm. For testing the effect of NPFF on the acquisition of EM-2-induced CPA, NPFF and EM-2 were co-injected on the conditioning days without drug treatment on the followed test day. To explore the effect of NPFF on the expression of EM-2-induced CPA, EM-2 was administered alone on the conditioning days, and NPFF was given 5 min before placement in the CPP apparatus on the test day. The results showed that NPFF (2.5, 5 and 10 nmol, i.c.v.) alone caused little place preference change. However, NPFF dose-dependently reversed the acquisition of CPA induced by 30 nmol EM-2 (i.c.v.). Similarly, the expression of EM-2-induced CPA was also reduced by NPFF. Moreover, the effects of NPFF on the acquisition and the expression of EM-2-induced CPA were completely blocked by the NPFF receptors antagonist RF9 (10 nmol, i.c.v.). However, central injection of NPFF neither changed the locomotor activity nor modified the locomotor action of EM-2. These data provide the first evidence for a functional interaction of the endogenous ligands for NPFF and MOP receptors, and further support an anti-opioid character of NPFF system.

Authors+Show Affiliations

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, and Institute of Physiology, School of Basic Medical Sciences, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23578757

Citation

Han, Zheng-lan, et al. "Neuropeptide FF Attenuates the Acquisition and the Expression of Conditioned Place Aversion to Endomorphin-2 in Mice." Behavioural Brain Research, vol. 248, 2013, pp. 51-6.
Han ZL, Wang ZL, Tang HZ, et al. Neuropeptide FF attenuates the acquisition and the expression of conditioned place aversion to endomorphin-2 in mice. Behav Brain Res. 2013;248:51-6.
Han, Z. L., Wang, Z. L., Tang, H. Z., Li, N., Fang, Q., Li, X. H., Yang, X. L., Zhang, X. Y., & Wang, R. (2013). Neuropeptide FF attenuates the acquisition and the expression of conditioned place aversion to endomorphin-2 in mice. Behavioural Brain Research, 248, 51-6. https://doi.org/10.1016/j.bbr.2013.03.046
Han ZL, et al. Neuropeptide FF Attenuates the Acquisition and the Expression of Conditioned Place Aversion to Endomorphin-2 in Mice. Behav Brain Res. 2013 Jul 1;248:51-6. PubMed PMID: 23578757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuropeptide FF attenuates the acquisition and the expression of conditioned place aversion to endomorphin-2 in mice. AU - Han,Zheng-lan, AU - Wang,Zi-long, AU - Tang,Hong-zhu, AU - Li,Ning, AU - Fang,Quan, AU - Li,Xu-hui, AU - Yang,Xiong-li, AU - Zhang,Xiao-yu, AU - Wang,Rui, Y1 - 2013/04/08/ PY - 2013/02/01/received PY - 2013/03/25/revised PY - 2013/03/28/accepted PY - 2013/4/13/entrez PY - 2013/4/13/pubmed PY - 2014/2/11/medline SP - 51 EP - 6 JF - Behavioural brain research JO - Behav Brain Res VL - 248 N2 - It has been demonstrated that the endogenous mu opioid (MOP) agonist endomorphin-2 (EM-2) produces conditioned place aversion (CPA) and in contrast, morphine exerts opposite action. Neuropeptide FF (NPFF) was reported to act as a functional antagonist of mu opioid receptor and to exert opioid-modulating activities. The present study examined the influence of NPFF on the rewarding action of EM-2, using the unbiased conditioned place preference (CPP) paradigm. For testing the effect of NPFF on the acquisition of EM-2-induced CPA, NPFF and EM-2 were co-injected on the conditioning days without drug treatment on the followed test day. To explore the effect of NPFF on the expression of EM-2-induced CPA, EM-2 was administered alone on the conditioning days, and NPFF was given 5 min before placement in the CPP apparatus on the test day. The results showed that NPFF (2.5, 5 and 10 nmol, i.c.v.) alone caused little place preference change. However, NPFF dose-dependently reversed the acquisition of CPA induced by 30 nmol EM-2 (i.c.v.). Similarly, the expression of EM-2-induced CPA was also reduced by NPFF. Moreover, the effects of NPFF on the acquisition and the expression of EM-2-induced CPA were completely blocked by the NPFF receptors antagonist RF9 (10 nmol, i.c.v.). However, central injection of NPFF neither changed the locomotor activity nor modified the locomotor action of EM-2. These data provide the first evidence for a functional interaction of the endogenous ligands for NPFF and MOP receptors, and further support an anti-opioid character of NPFF system. SN - 1872-7549 UR - https://www.unboundmedicine.com/medline/citation/23578757/Neuropeptide_FF_attenuates_the_acquisition_and_the_expression_of_conditioned_place_aversion_to_endomorphin_2_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-4328(13)00188-5 DB - PRIME DP - Unbound Medicine ER -