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Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP(+)-dependent xylitol dehydrogenase.
J Biotechnol. 2013 Jun 10; 165(3-4):153-6.JB

Abstract

The xylose-fermenting recombinant Saccharomyces cerevisiae and its improvement have been studied extensively. The redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH) is thought to be an important factor in effective xylose fermentation. Using protein engineering, we previously successfully reduced xylitol accumulation and improved ethanol production by reversing the dependency of XDH from NAD(+) to NADP(+). We also constructed a set of novel strictly NADPH-dependent XR from Pichia stipitis by site-directed mutagenesis. In the present study, we constructed a set of recombinant S. cerevisiae carrying a novel set of mutated strictly NADPH-dependent XR and NADP(+)-dependent XDH genes with overexpression of endogenous xylulokinase (XK) to study the effects of complete NADPH/NADP(+) recycling on ethanol fermentation and xylitol accumulation. All mutated strains demonstrated reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain. Moreover, compared with the control strain, the two strains showed 20% and 10% improvement in ethanol production.

Authors+Show Affiliations

Faculty of Science, Al-Azhar University, Assiut Branch, Assiut 71524, Egypt.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23578809

Citation

Khattab, Sadat Mohammad Rezq, et al. "Boost in Bioethanol Production Using Recombinant Saccharomyces Cerevisiae With Mutated Strictly NADPH-dependent Xylose Reductase and NADP(+)-dependent Xylitol Dehydrogenase." Journal of Biotechnology, vol. 165, no. 3-4, 2013, pp. 153-6.
Khattab SM, Saimura M, Kodaki T. Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP(+)-dependent xylitol dehydrogenase. J Biotechnol. 2013;165(3-4):153-6.
Khattab, S. M., Saimura, M., & Kodaki, T. (2013). Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP(+)-dependent xylitol dehydrogenase. Journal of Biotechnology, 165(3-4), 153-6. https://doi.org/10.1016/j.jbiotec.2013.03.009
Khattab SM, Saimura M, Kodaki T. Boost in Bioethanol Production Using Recombinant Saccharomyces Cerevisiae With Mutated Strictly NADPH-dependent Xylose Reductase and NADP(+)-dependent Xylitol Dehydrogenase. J Biotechnol. 2013 Jun 10;165(3-4):153-6. PubMed PMID: 23578809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP(+)-dependent xylitol dehydrogenase. AU - Khattab,Sadat Mohammad Rezq, AU - Saimura,Masayuki, AU - Kodaki,Tsutomu, Y1 - 2013/04/08/ PY - 2012/11/27/received PY - 2013/02/12/revised PY - 2013/03/19/accepted PY - 2013/4/13/entrez PY - 2013/4/13/pubmed PY - 2013/11/5/medline SP - 153 EP - 6 JF - Journal of biotechnology JO - J Biotechnol VL - 165 IS - 3-4 N2 - The xylose-fermenting recombinant Saccharomyces cerevisiae and its improvement have been studied extensively. The redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH) is thought to be an important factor in effective xylose fermentation. Using protein engineering, we previously successfully reduced xylitol accumulation and improved ethanol production by reversing the dependency of XDH from NAD(+) to NADP(+). We also constructed a set of novel strictly NADPH-dependent XR from Pichia stipitis by site-directed mutagenesis. In the present study, we constructed a set of recombinant S. cerevisiae carrying a novel set of mutated strictly NADPH-dependent XR and NADP(+)-dependent XDH genes with overexpression of endogenous xylulokinase (XK) to study the effects of complete NADPH/NADP(+) recycling on ethanol fermentation and xylitol accumulation. All mutated strains demonstrated reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain. Moreover, compared with the control strain, the two strains showed 20% and 10% improvement in ethanol production. SN - 1873-4863 UR - https://www.unboundmedicine.com/medline/citation/23578809/Boost_in_bioethanol_production_using_recombinant_Saccharomyces_cerevisiae_with_mutated_strictly_NADPH_dependent_xylose_reductase_and_NADP_+__dependent_xylitol_dehydrogenase_ DB - PRIME DP - Unbound Medicine ER -