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Peptidergic signaling in Calanus finmarchicus (Crustacea, Copepoda): in silico identification of putative peptide hormones and their receptors using a de novo assembled transcriptome.
Gen Comp Endocrinol. 2013 Jun 15; 187:117-35.GC

Abstract

The copepod Calanus finmarchicus is the most abundant zooplankton species in the North Atlantic. While the life history of this crustacean is well studied, little is known about its peptidergic signaling systems despite the fact that these pathways are undoubtedly important components of its physiological/behavioral control systems. Here we have generated and used a de novo assembled transcriptome for C. finmarchicus (206,041 sequences in total) to identify peptide precursor proteins and receptors. Using known protein queries, 34 transcripts encoding peptide preprohormones and 18 encoding peptide receptors were identified. Using a combination of online software programs and homology to known arthropod isoforms, 148 mature peptides were predicted from the deduced precursors, including members of the allatostatin-A, allatostatin-B, allatostatin-C, bursicon, crustacean cardioactive peptide (CCAP), crustacean hyperglycemic hormone, diuretic hormone 31 (DH31), diuretic hormone 44 (DH44), FMRFamide-like peptide (myosuppressin, neuropeptide F [NPF] and extended FL/IRFamide subfamilies), leucokinin, neuroparsin, orcokinin, orcomyotropin, periviscerokinin, RYamide and tachykinin-related peptide (TRP) families. The identified receptors included ones for allatostatin-A, allatostatin-C, bursicon, CCAP, DH31, DH44, ecdysis-triggering hormone, NPF, short NPF, FMRFamide, insulin-like peptide, leucokinin, periviscerokinin, pigment dispersing hormone, and TRP. Developmental profiling of the identified transcripts in embryos, early nauplii, late nauplii, early copepodites, late copepodites, and adult females was also undertaken, with all showing the highest expression levels in the naupliar and copepodite stages. Collectively, these data radically expand the catalog of known C. finmarchicus peptidergic signaling proteins and provide a foundation for experiments directed at understanding the physiological roles served by them in this species.

Authors+Show Affiliations

Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA. crabman@pbrc.hawaii.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23578900

Citation

Christie, Andrew E., et al. "Peptidergic Signaling in Calanus Finmarchicus (Crustacea, Copepoda): in Silico Identification of Putative Peptide Hormones and Their Receptors Using a De Novo Assembled Transcriptome." General and Comparative Endocrinology, vol. 187, 2013, pp. 117-35.
Christie AE, Roncalli V, Wu LS, et al. Peptidergic signaling in Calanus finmarchicus (Crustacea, Copepoda): in silico identification of putative peptide hormones and their receptors using a de novo assembled transcriptome. Gen Comp Endocrinol. 2013;187:117-35.
Christie, A. E., Roncalli, V., Wu, L. S., Ganote, C. L., Doak, T., & Lenz, P. H. (2013). Peptidergic signaling in Calanus finmarchicus (Crustacea, Copepoda): in silico identification of putative peptide hormones and their receptors using a de novo assembled transcriptome. General and Comparative Endocrinology, 187, 117-35. https://doi.org/10.1016/j.ygcen.2013.03.018
Christie AE, et al. Peptidergic Signaling in Calanus Finmarchicus (Crustacea, Copepoda): in Silico Identification of Putative Peptide Hormones and Their Receptors Using a De Novo Assembled Transcriptome. Gen Comp Endocrinol. 2013 Jun 15;187:117-35. PubMed PMID: 23578900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Peptidergic signaling in Calanus finmarchicus (Crustacea, Copepoda): in silico identification of putative peptide hormones and their receptors using a de novo assembled transcriptome. AU - Christie,Andrew E, AU - Roncalli,Vittoria, AU - Wu,Le-Shin, AU - Ganote,Carrie L, AU - Doak,Thomas, AU - Lenz,Petra H, Y1 - 2013/04/08/ PY - 2013/01/17/received PY - 2013/03/15/revised PY - 2013/03/19/accepted PY - 2013/4/13/entrez PY - 2013/4/13/pubmed PY - 2014/1/16/medline SP - 117 EP - 35 JF - General and comparative endocrinology JO - Gen Comp Endocrinol VL - 187 N2 - The copepod Calanus finmarchicus is the most abundant zooplankton species in the North Atlantic. While the life history of this crustacean is well studied, little is known about its peptidergic signaling systems despite the fact that these pathways are undoubtedly important components of its physiological/behavioral control systems. Here we have generated and used a de novo assembled transcriptome for C. finmarchicus (206,041 sequences in total) to identify peptide precursor proteins and receptors. Using known protein queries, 34 transcripts encoding peptide preprohormones and 18 encoding peptide receptors were identified. Using a combination of online software programs and homology to known arthropod isoforms, 148 mature peptides were predicted from the deduced precursors, including members of the allatostatin-A, allatostatin-B, allatostatin-C, bursicon, crustacean cardioactive peptide (CCAP), crustacean hyperglycemic hormone, diuretic hormone 31 (DH31), diuretic hormone 44 (DH44), FMRFamide-like peptide (myosuppressin, neuropeptide F [NPF] and extended FL/IRFamide subfamilies), leucokinin, neuroparsin, orcokinin, orcomyotropin, periviscerokinin, RYamide and tachykinin-related peptide (TRP) families. The identified receptors included ones for allatostatin-A, allatostatin-C, bursicon, CCAP, DH31, DH44, ecdysis-triggering hormone, NPF, short NPF, FMRFamide, insulin-like peptide, leucokinin, periviscerokinin, pigment dispersing hormone, and TRP. Developmental profiling of the identified transcripts in embryos, early nauplii, late nauplii, early copepodites, late copepodites, and adult females was also undertaken, with all showing the highest expression levels in the naupliar and copepodite stages. Collectively, these data radically expand the catalog of known C. finmarchicus peptidergic signaling proteins and provide a foundation for experiments directed at understanding the physiological roles served by them in this species. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/23578900/Peptidergic_signaling_in_Calanus_finmarchicus__Crustacea_Copepoda_:_in_silico_identification_of_putative_peptide_hormones_and_their_receptors_using_a_de_novo_assembled_transcriptome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(13)00150-0 DB - PRIME DP - Unbound Medicine ER -