Tags

Type your tag names separated by a space and hit enter

Serum diamine oxidase activity as a diagnostic test for histamine intolerance.
Wien Klin Wochenschr. 2013 May; 125(9-10):239-43.WK

Abstract

BACKGROUND

Histamine intolerance (HIT) is characterized by an imbalance between histamine intake and the capacity for histamine degradation. The main enzyme for metabolizing ingested histamine is diamine oxidase (DAO). Determining DAO activity in serum may be useful in diagnosing HIT.

METHODS

Over a period of 3.5 years we recruited 316 subjects with clinically suspected HIT and 55 healthy controls. Serum DAO activity was measured with a quantitative enzyme immunoassay. Twenty patients with highly reduced DAO activity went on a histamine-free diet for 6-12 months. Afterwards, their DAO activity was determined again.

RESULTS

We found that DAO activity was significantly lower in patients than in healthy control subjects (P < 0.0001). Furthermore, 54 patients had highly reduced serum DAO activity (< 40 HDU/ml). Their main symptoms involved the skin, gastrointestinal tract, respiratory system, and eyes. In all the 20 patients with highly reduced DAO activity, the main clinical symptoms typical of histamine intolerance disappeared after they adopted a histamine-free diet. Furthermore, the serum DAO activity values measured increased significantly (P < 0.0001).

CONCLUSIONS

Our results suggest that determining DAO activity in serum is a useful tool in diagnosing HIT. Furthermore, our results showed the benefit of a histamine-free diet because after the diet the majority of symptoms disappeared and the serum DAO activity significantly increased.

Authors+Show Affiliations

University Clinic of Respiratory and Allergic Diseases Golnik, Golnik 36, 4204 Golnik, Slovenia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23579881

Citation

Mušič, Ema, et al. "Serum Diamine Oxidase Activity as a Diagnostic Test for Histamine Intolerance." Wiener Klinische Wochenschrift, vol. 125, no. 9-10, 2013, pp. 239-43.
Mušič E, Korošec P, Šilar M, et al. Serum diamine oxidase activity as a diagnostic test for histamine intolerance. Wien Klin Wochenschr. 2013;125(9-10):239-43.
Mušič, E., Korošec, P., Šilar, M., Adamič, K., Košnik, M., & Rijavec, M. (2013). Serum diamine oxidase activity as a diagnostic test for histamine intolerance. Wiener Klinische Wochenschrift, 125(9-10), 239-43. https://doi.org/10.1007/s00508-013-0354-y
Mušič E, et al. Serum Diamine Oxidase Activity as a Diagnostic Test for Histamine Intolerance. Wien Klin Wochenschr. 2013;125(9-10):239-43. PubMed PMID: 23579881.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum diamine oxidase activity as a diagnostic test for histamine intolerance. AU - Mušič,Ema, AU - Korošec,Peter, AU - Šilar,Mira, AU - Adamič,Katja, AU - Košnik,Mitja, AU - Rijavec,Matija, Y1 - 2013/04/12/ PY - 2012/03/29/received PY - 2013/03/20/accepted PY - 2013/4/13/entrez PY - 2013/4/13/pubmed PY - 2014/1/25/medline SP - 239 EP - 43 JF - Wiener klinische Wochenschrift JO - Wien. Klin. Wochenschr. VL - 125 IS - 9-10 N2 - BACKGROUND: Histamine intolerance (HIT) is characterized by an imbalance between histamine intake and the capacity for histamine degradation. The main enzyme for metabolizing ingested histamine is diamine oxidase (DAO). Determining DAO activity in serum may be useful in diagnosing HIT. METHODS: Over a period of 3.5 years we recruited 316 subjects with clinically suspected HIT and 55 healthy controls. Serum DAO activity was measured with a quantitative enzyme immunoassay. Twenty patients with highly reduced DAO activity went on a histamine-free diet for 6-12 months. Afterwards, their DAO activity was determined again. RESULTS: We found that DAO activity was significantly lower in patients than in healthy control subjects (P < 0.0001). Furthermore, 54 patients had highly reduced serum DAO activity (< 40 HDU/ml). Their main symptoms involved the skin, gastrointestinal tract, respiratory system, and eyes. In all the 20 patients with highly reduced DAO activity, the main clinical symptoms typical of histamine intolerance disappeared after they adopted a histamine-free diet. Furthermore, the serum DAO activity values measured increased significantly (P < 0.0001). CONCLUSIONS: Our results suggest that determining DAO activity in serum is a useful tool in diagnosing HIT. Furthermore, our results showed the benefit of a histamine-free diet because after the diet the majority of symptoms disappeared and the serum DAO activity significantly increased. SN - 1613-7671 UR - https://www.unboundmedicine.com/medline/citation/23579881/Serum_diamine_oxidase_activity_as_a_diagnostic_test_for_histamine_intolerance_ L2 - https://dx.doi.org/10.1007/s00508-013-0354-y DB - PRIME DP - Unbound Medicine ER -