Tags

Type your tag names separated by a space and hit enter

Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK.
J Antimicrob Chemother. 2013 Jul; 68(7):1543-50.JA

Abstract

OBJECTIVES

16S rRNA methyltransferases are an emerging mechanism conferring high-level resistance to clinically relevant aminoglycosides and have been associated with important mechanisms such as NDM-1. We sought genes encoding these enzymes in isolates highly resistant (MIC >200 mg/L) to gentamicin and amikacin from an Indian hospital and we additionally screened for the novel RmtF enzyme in 132 UK isolates containing NDM.

METHODS

All highly aminoglycoside-resistant isolates were screened for armA and rmtA-E by PCR, with cloning experiments performed for isolates negative for these genes. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was used to determine the methylation target of the novel RmtF methyltransferase. RmtF-bearing strains were characterized further, including susceptibility testing, PFGE, electroporation, PCR-based replicon typing and multilocus sequence typing of rmtF-bearing plasmids.

RESULTS

High-level aminoglycoside resistance was detected in 140/1000 (14%) consecutive isolates of Enterobacteriaceae from India. ArmA, RmtB and RmtC were identified among 46%, 20% and 27% of these isolates, respectively. The novel rmtF gene was detected in 34 aminoglycoside-resistant isolates (overall prevalence 3.4%), most (59%) of which also possessed a bla(NDM) gene; rmtF was detected in 6 NDM producers from the UK. It was found on different plasmid backbones. Four and two isolates showed resistance to tigecycline and colistin, respectively.

CONCLUSIONS

RmtF was often found in association with NDM in members of the Enterobacteriaceae and on diverse plasmids. It is of clinical concern that the RmtF- and NDM-positive strains identified here show additional resistance to tigecycline and colistin, current drugs of last resort for the treatment of serious bacterial infections.

Authors+Show Affiliations

Department of Animal Health and VISAVET, Universidad Complutense de Madrid, Madrid, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23580560

Citation

Hidalgo, Laura, et al. "Association of the Novel Aminoglycoside Resistance Determinant RmtF With NDM Carbapenemase in Enterobacteriaceae Isolated in India and the UK." The Journal of Antimicrobial Chemotherapy, vol. 68, no. 7, 2013, pp. 1543-50.
Hidalgo L, Hopkins KL, Gutierrez B, et al. Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK. J Antimicrob Chemother. 2013;68(7):1543-50.
Hidalgo, L., Hopkins, K. L., Gutierrez, B., Ovejero, C. M., Shukla, S., Douthwaite, S., Prasad, K. N., Woodford, N., & Gonzalez-Zorn, B. (2013). Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK. The Journal of Antimicrobial Chemotherapy, 68(7), 1543-50. https://doi.org/10.1093/jac/dkt078
Hidalgo L, et al. Association of the Novel Aminoglycoside Resistance Determinant RmtF With NDM Carbapenemase in Enterobacteriaceae Isolated in India and the UK. J Antimicrob Chemother. 2013;68(7):1543-50. PubMed PMID: 23580560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of the novel aminoglycoside resistance determinant RmtF with NDM carbapenemase in Enterobacteriaceae isolated in India and the UK. AU - Hidalgo,Laura, AU - Hopkins,Katie L, AU - Gutierrez,Belen, AU - Ovejero,Cristina M, AU - Shukla,Suruchi, AU - Douthwaite,Stephen, AU - Prasad,Kashi N, AU - Woodford,Neil, AU - Gonzalez-Zorn,Bruno, Y1 - 2013/04/11/ PY - 2013/4/13/entrez PY - 2013/4/13/pubmed PY - 2013/12/29/medline KW - 16S rRNA methyltransferases KW - antimicrobial KW - resistance SP - 1543 EP - 50 JF - The Journal of antimicrobial chemotherapy JO - J. Antimicrob. Chemother. VL - 68 IS - 7 N2 - OBJECTIVES: 16S rRNA methyltransferases are an emerging mechanism conferring high-level resistance to clinically relevant aminoglycosides and have been associated with important mechanisms such as NDM-1. We sought genes encoding these enzymes in isolates highly resistant (MIC >200 mg/L) to gentamicin and amikacin from an Indian hospital and we additionally screened for the novel RmtF enzyme in 132 UK isolates containing NDM. METHODS: All highly aminoglycoside-resistant isolates were screened for armA and rmtA-E by PCR, with cloning experiments performed for isolates negative for these genes. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was used to determine the methylation target of the novel RmtF methyltransferase. RmtF-bearing strains were characterized further, including susceptibility testing, PFGE, electroporation, PCR-based replicon typing and multilocus sequence typing of rmtF-bearing plasmids. RESULTS: High-level aminoglycoside resistance was detected in 140/1000 (14%) consecutive isolates of Enterobacteriaceae from India. ArmA, RmtB and RmtC were identified among 46%, 20% and 27% of these isolates, respectively. The novel rmtF gene was detected in 34 aminoglycoside-resistant isolates (overall prevalence 3.4%), most (59%) of which also possessed a bla(NDM) gene; rmtF was detected in 6 NDM producers from the UK. It was found on different plasmid backbones. Four and two isolates showed resistance to tigecycline and colistin, respectively. CONCLUSIONS: RmtF was often found in association with NDM in members of the Enterobacteriaceae and on diverse plasmids. It is of clinical concern that the RmtF- and NDM-positive strains identified here show additional resistance to tigecycline and colistin, current drugs of last resort for the treatment of serious bacterial infections. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/23580560/Association_of_the_novel_aminoglycoside_resistance_determinant_RmtF_with_NDM_carbapenemase_in_Enterobacteriaceae_isolated_in_India_and_the_UK_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkt078 DB - PRIME DP - Unbound Medicine ER -