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Satellite cell functional alterations following cutaneous burn in rats include an increase in their osteogenic potential.
J Surg Res. 2013 Oct; 184(2):e9-16.JS

Abstract

BACKGROUND

Significant consequences of severe burn include skeletal muscle atrophy and heterotopic ossification (HO). The cellular mechanisms underlying either of these conditions are not known. Whether the functionality of satellite cells stem cells resident in skeletal muscle is affected by changes in circulatory factors following burn was determined to better understand their role in atrophy and HO.

MATERIALS AND METHODS

Serum (20%) from sham-treated animals or burned animals (40% total body surface area full-thickness burn) was used to culture satellite cells isolated from either sham or burn animals. Satellite cells were separated based on fiber type (i.e., fast-twitch or slow-twitch in some cases). To gain greater insight into the potential role for satellite cells in controlling muscle mass following burn, the effect of serum taken from burn animals on satellite cell proliferation, migration, and myogenic differentiation was evaluated. Osteogenic differentiation was assessed to evaluate the potential of satellite cells to contribute to HO.

RESULTS

Burn serum (BS) increased the proliferative capacity of cells from fast-twitch muscle, and the migratory capacity of satellite cells taken from both fast- and slow-twitch muscles. BS increased both the myogenic and osteogenic differentiation of satellite cells taken from both sham and burn animals.

CONCLUSIONS

The unexpected increase in myogenic functionality of satellite cells with BS is difficult to rectify, given the degree of atrophy that occurs. However, the increased osteogenic capacity of satellite cells with BS suggests they may play a role in burn-induced HO.

Authors+Show Affiliations

Extremity Trauma and Regenerative Medicine, US Army Institute of Surgical Research, Fort Sam Houston, Texas. Electronic address: xiaowu.wu.ctr@mail.mil.No affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

23582758

Citation

Wu, Xiaowu, and Christopher R. Rathbone. "Satellite Cell Functional Alterations Following Cutaneous Burn in Rats Include an Increase in Their Osteogenic Potential." The Journal of Surgical Research, vol. 184, no. 2, 2013, pp. e9-16.
Wu X, Rathbone CR. Satellite cell functional alterations following cutaneous burn in rats include an increase in their osteogenic potential. J Surg Res. 2013;184(2):e9-16.
Wu, X., & Rathbone, C. R. (2013). Satellite cell functional alterations following cutaneous burn in rats include an increase in their osteogenic potential. The Journal of Surgical Research, 184(2), e9-16. https://doi.org/10.1016/j.jss.2013.03.046
Wu X, Rathbone CR. Satellite Cell Functional Alterations Following Cutaneous Burn in Rats Include an Increase in Their Osteogenic Potential. J Surg Res. 2013;184(2):e9-16. PubMed PMID: 23582758.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Satellite cell functional alterations following cutaneous burn in rats include an increase in their osteogenic potential. AU - Wu,Xiaowu, AU - Rathbone,Christopher R, Y1 - 2013/04/05/ PY - 2013/01/13/received PY - 2013/03/05/revised PY - 2013/03/14/accepted PY - 2013/4/16/entrez PY - 2013/4/16/pubmed PY - 2014/2/5/medline KW - Atrophy KW - Heterotopic ossification KW - Muscle precursor cell KW - Skeletal muscle KW - Thermal injury SP - e9 EP - 16 JF - The Journal of surgical research JO - J Surg Res VL - 184 IS - 2 N2 - BACKGROUND: Significant consequences of severe burn include skeletal muscle atrophy and heterotopic ossification (HO). The cellular mechanisms underlying either of these conditions are not known. Whether the functionality of satellite cells stem cells resident in skeletal muscle is affected by changes in circulatory factors following burn was determined to better understand their role in atrophy and HO. MATERIALS AND METHODS: Serum (20%) from sham-treated animals or burned animals (40% total body surface area full-thickness burn) was used to culture satellite cells isolated from either sham or burn animals. Satellite cells were separated based on fiber type (i.e., fast-twitch or slow-twitch in some cases). To gain greater insight into the potential role for satellite cells in controlling muscle mass following burn, the effect of serum taken from burn animals on satellite cell proliferation, migration, and myogenic differentiation was evaluated. Osteogenic differentiation was assessed to evaluate the potential of satellite cells to contribute to HO. RESULTS: Burn serum (BS) increased the proliferative capacity of cells from fast-twitch muscle, and the migratory capacity of satellite cells taken from both fast- and slow-twitch muscles. BS increased both the myogenic and osteogenic differentiation of satellite cells taken from both sham and burn animals. CONCLUSIONS: The unexpected increase in myogenic functionality of satellite cells with BS is difficult to rectify, given the degree of atrophy that occurs. However, the increased osteogenic capacity of satellite cells with BS suggests they may play a role in burn-induced HO. SN - 1095-8673 UR - https://www.unboundmedicine.com/medline/citation/23582758/Satellite_cell_functional_alterations_following_cutaneous_burn_in_rats_include_an_increase_in_their_osteogenic_potential_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-4804(13)00240-0 DB - PRIME DP - Unbound Medicine ER -