Tags

Type your tag names separated by a space and hit enter

Seasonal variation in the toxicological properties of size-segregated indoor and outdoor air particulate matter.
Toxicol In Vitro. 2013 Aug; 27(5):1550-61.TV

Abstract

Ambient air particulate matter (PM) as well as microbial contaminants in the indoor air are known to cause severe adverse health effects. It has been shown that there is a clear seasonal variation in the potency of outdoor air particles to evoke inflammation and cytotoxicity. However, the role of outdoor sources in the indoor air quality, especially on its toxicological properties, remains largely unknown. In this study, we collected size segregated (PM10-2.5, PM2.5-0.2 and PM0.2) particulate samples with a high volume cascade impactor (HVCI) on polyurethane foam and fluoropore membrane filters. The samples were collected during four different seasons simultaneously from indoor and outdoor air. Thereafter, the samples were weighed and extracted with methanol from the filters before undergoing toxicological analyses. Mouse macrophages (RAW264.7) were exposed to particulate sample doses of 50, 150 and 300μg/ml for 24h. Thereafter, the levels of the proinflammatory cytokine (TNF-α), NO-production, cytotoxicity (MTT-test) and changes in the cell cycle (SubG1, G1, S and G2/M phases) were investigated. PM10-2.5 particles evoked the highest inflammatory and cytotoxic responses. Instead, PM2.5-0.2 samples exerted the greatest effect on apoptotic activity in the macrophages. With respect to the outdoor air samples, particles collected during warm seasons had a stronger potency to induce inflammatory and cytotoxic responses, whereas no such clear effect was seen with the corresponding indoor air samples. Outdoor air samples were associated with higher inflammatory potential, whereas indoor air samples had overall higher cytotoxic properties. This indicates that the outdoor air has a limited influence on the indoor air quality in a modern house. Thus, the indoor sources dominate the toxicological responses obtained from samples collected inside house.

Authors+Show Affiliations

University of Eastern Finland, Department of Environmental Science, Kuopio, Finland. mikko.happo@uef.fiNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23583641

Citation

Happo, Mikko, et al. "Seasonal Variation in the Toxicological Properties of Size-segregated Indoor and Outdoor Air Particulate Matter." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 27, no. 5, 2013, pp. 1550-61.
Happo M, Markkanen A, Markkanen P, et al. Seasonal variation in the toxicological properties of size-segregated indoor and outdoor air particulate matter. Toxicol In Vitro. 2013;27(5):1550-61.
Happo, M., Markkanen, A., Markkanen, P., Jalava, P., Kuuspalo, K., Leskinen, A., Sippula, O., Lehtinen, K., Jokiniemi, J., & Hirvonen, M. R. (2013). Seasonal variation in the toxicological properties of size-segregated indoor and outdoor air particulate matter. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 27(5), 1550-61. https://doi.org/10.1016/j.tiv.2013.04.001
Happo M, et al. Seasonal Variation in the Toxicological Properties of Size-segregated Indoor and Outdoor Air Particulate Matter. Toxicol In Vitro. 2013;27(5):1550-61. PubMed PMID: 23583641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Seasonal variation in the toxicological properties of size-segregated indoor and outdoor air particulate matter. AU - Happo,Mikko, AU - Markkanen,Ari, AU - Markkanen,Piia, AU - Jalava,Pasi, AU - Kuuspalo,Kari, AU - Leskinen,Ari, AU - Sippula,Olli, AU - Lehtinen,Kari, AU - Jokiniemi,Jorma, AU - Hirvonen,Maija-Riitta, Y1 - 2013/04/11/ PY - 2012/11/16/received PY - 2013/03/12/revised PY - 2013/04/03/accepted PY - 2013/4/16/entrez PY - 2013/4/16/pubmed PY - 2013/12/16/medline SP - 1550 EP - 61 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 27 IS - 5 N2 - Ambient air particulate matter (PM) as well as microbial contaminants in the indoor air are known to cause severe adverse health effects. It has been shown that there is a clear seasonal variation in the potency of outdoor air particles to evoke inflammation and cytotoxicity. However, the role of outdoor sources in the indoor air quality, especially on its toxicological properties, remains largely unknown. In this study, we collected size segregated (PM10-2.5, PM2.5-0.2 and PM0.2) particulate samples with a high volume cascade impactor (HVCI) on polyurethane foam and fluoropore membrane filters. The samples were collected during four different seasons simultaneously from indoor and outdoor air. Thereafter, the samples were weighed and extracted with methanol from the filters before undergoing toxicological analyses. Mouse macrophages (RAW264.7) were exposed to particulate sample doses of 50, 150 and 300μg/ml for 24h. Thereafter, the levels of the proinflammatory cytokine (TNF-α), NO-production, cytotoxicity (MTT-test) and changes in the cell cycle (SubG1, G1, S and G2/M phases) were investigated. PM10-2.5 particles evoked the highest inflammatory and cytotoxic responses. Instead, PM2.5-0.2 samples exerted the greatest effect on apoptotic activity in the macrophages. With respect to the outdoor air samples, particles collected during warm seasons had a stronger potency to induce inflammatory and cytotoxic responses, whereas no such clear effect was seen with the corresponding indoor air samples. Outdoor air samples were associated with higher inflammatory potential, whereas indoor air samples had overall higher cytotoxic properties. This indicates that the outdoor air has a limited influence on the indoor air quality in a modern house. Thus, the indoor sources dominate the toxicological responses obtained from samples collected inside house. SN - 1879-3177 UR - https://www.unboundmedicine.com/medline/citation/23583641/Seasonal_variation_in_the_toxicological_properties_of_size_segregated_indoor_and_outdoor_air_particulate_matter_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(13)00088-X DB - PRIME DP - Unbound Medicine ER -