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Advanced oxidative and glycoxidative protein damage markers in the elderly with type 2 diabetes.

Abstract

We aimed to explore the association of advanced oxidation and advanced glycation of proteins, and their interrelations with endothelial nitric oxide synthesis, oxidative stress, metabolic profile as well as other atherosclerotic risk markers in prediabetic and diabetic elderly subjects. Advanced glycation end products (AGEs), advanced oxidation protein products (AOPPs), low-density lipoprotein susceptibility to oxidation (oxLDL) and nitric oxide metabolic pathway products (NOx) were assessed in subjects with impaired fasting glucose (prediabetes, IFG; n=90), and type 2 diabetes mellitus (T2DM, n=95) versus control subjects (n=88). Higher levels of AOPPs, AGEs, oxLDL, NOx, atherosclerosis risk markers, and insulin resistance were pointed out in IFG and T2DM groups compared with control. Strong positive associations (p<0.01) of AGEs with fasting glucose and HbA1c were found in both hyperglycemic groups, whereas AOPPs were significantly correlated (p<0.01) only in T2DM. In T2DM, AGEs and AOPPs significantly (p<0.01) correlated with insulin resistance index HOMA-IR, oxLDL and small LDL particle size (TG/HDL-C), and positively with NOx. Direct associations of AGEs and AOPPs with TC/HDL-C and oxLDL/HDL-C, and AGEs-AOPPs interrelations (p<0.01) were identified in IFG and T2DM groups. AGEs and AOPPs in combination with oxLDL and NOx could be important biomarkers for evaluating the association between diabetes and atherosclerotic disorders in aging diabetic patients.

BIOLOGICAL SIGNIFICANCE

In the present study we have made an attempt to approach the biological and clinical significance of the oxidative and glycoxidative protein damage, in subjects with prediabetes and type-2 diabetes mellitus. AGEs and AOPPs in combination with oxLDL and NOx appear to be important biomarkers for evaluating the association between diabetes and atherosclerotic disorders in aging diabetic patients. More importantly, this cluster of biomarkers that links the short term, "real time" metabolic impairment parameters (NOx, serum glucose, HOMA-IR, serum lipid profile) and the "metabolic memory" markers resulting from the long-term hyperglycemia and hyperlipidemia-induced oxidative stress (HbA1c, AGEs, AOPPs and oxLDL), could be valuable in predicting not only vascular complications in T2DM, but also the onset of diabetes, hence enabling therapeutic interventions from the early stages of diabetes. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Ana Aslan - National Institute of Gerontology and Geriatrics, Bucharest, Romania; Carol Davila - University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Biochemistry, Bucharest, Romania. Electronic address: danielagrdnr@yahoo.com.

    , ,

    Source

    Journal of proteomics 92: 2013 Oct 30 pg 313-22

    MeSH

    Aged
    Aging
    Atherosclerosis
    Biomarkers
    Diabetes Mellitus, Type 2
    Female
    Glycation End Products, Advanced
    Humans
    Insulin Resistance
    Lipoproteins, LDL
    Male
    Middle Aged
    Nitric Oxide
    Oxidative Stress

    Pub Type(s)

    Clinical Trial
    Journal Article

    Language

    eng

    PubMed ID

    23587667

    Citation

    Gradinaru, Daniela, et al. "Advanced Oxidative and Glycoxidative Protein Damage Markers in the Elderly With Type 2 Diabetes." Journal of Proteomics, vol. 92, 2013, pp. 313-22.
    Gradinaru D, Borsa C, Ionescu C, et al. Advanced oxidative and glycoxidative protein damage markers in the elderly with type 2 diabetes. J Proteomics. 2013;92:313-22.
    Gradinaru, D., Borsa, C., Ionescu, C., & Margina, D. (2013). Advanced oxidative and glycoxidative protein damage markers in the elderly with type 2 diabetes. Journal of Proteomics, 92, pp. 313-22. doi:10.1016/j.jprot.2013.03.034.
    Gradinaru D, et al. Advanced Oxidative and Glycoxidative Protein Damage Markers in the Elderly With Type 2 Diabetes. J Proteomics. 2013 Oct 30;92:313-22. PubMed PMID: 23587667.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Advanced oxidative and glycoxidative protein damage markers in the elderly with type 2 diabetes. AU - Gradinaru,Daniela, AU - Borsa,Claudia, AU - Ionescu,Cristina, AU - Margina,Denisa, Y1 - 2013/04/13/ PY - 2013/01/06/received PY - 2013/03/03/revised PY - 2013/03/23/accepted PY - 2013/4/17/entrez PY - 2013/4/17/pubmed PY - 2014/6/3/medline KW - Advanced glycation end products (AGEs) KW - Advanced oxidation protein products (AOPPs) KW - Elderly KW - Oxidative stress KW - Prediabetes KW - Type 2 diabetes SP - 313 EP - 22 JF - Journal of proteomics JO - J Proteomics VL - 92 N2 - UNLABELLED: We aimed to explore the association of advanced oxidation and advanced glycation of proteins, and their interrelations with endothelial nitric oxide synthesis, oxidative stress, metabolic profile as well as other atherosclerotic risk markers in prediabetic and diabetic elderly subjects. Advanced glycation end products (AGEs), advanced oxidation protein products (AOPPs), low-density lipoprotein susceptibility to oxidation (oxLDL) and nitric oxide metabolic pathway products (NOx) were assessed in subjects with impaired fasting glucose (prediabetes, IFG; n=90), and type 2 diabetes mellitus (T2DM, n=95) versus control subjects (n=88). Higher levels of AOPPs, AGEs, oxLDL, NOx, atherosclerosis risk markers, and insulin resistance were pointed out in IFG and T2DM groups compared with control. Strong positive associations (p<0.01) of AGEs with fasting glucose and HbA1c were found in both hyperglycemic groups, whereas AOPPs were significantly correlated (p<0.01) only in T2DM. In T2DM, AGEs and AOPPs significantly (p<0.01) correlated with insulin resistance index HOMA-IR, oxLDL and small LDL particle size (TG/HDL-C), and positively with NOx. Direct associations of AGEs and AOPPs with TC/HDL-C and oxLDL/HDL-C, and AGEs-AOPPs interrelations (p<0.01) were identified in IFG and T2DM groups. AGEs and AOPPs in combination with oxLDL and NOx could be important biomarkers for evaluating the association between diabetes and atherosclerotic disorders in aging diabetic patients. BIOLOGICAL SIGNIFICANCE: In the present study we have made an attempt to approach the biological and clinical significance of the oxidative and glycoxidative protein damage, in subjects with prediabetes and type-2 diabetes mellitus. AGEs and AOPPs in combination with oxLDL and NOx appear to be important biomarkers for evaluating the association between diabetes and atherosclerotic disorders in aging diabetic patients. More importantly, this cluster of biomarkers that links the short term, "real time" metabolic impairment parameters (NOx, serum glucose, HOMA-IR, serum lipid profile) and the "metabolic memory" markers resulting from the long-term hyperglycemia and hyperlipidemia-induced oxidative stress (HbA1c, AGEs, AOPPs and oxLDL), could be valuable in predicting not only vascular complications in T2DM, but also the onset of diabetes, hence enabling therapeutic interventions from the early stages of diabetes. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/23587667/Advanced_oxidative_and_glycoxidative_protein_damage_markers_in_the_elderly_with_type_2_diabetes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(13)00181-4 DB - PRIME DP - Unbound Medicine ER -