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Characteristics of basal cytokeratin expression in breast cancer.
Breast Cancer Res Treat. 2013 May; 139(1):23-37.BC

Abstract

Breast cancer is recognised to be a heterogeneous disease and the second most common cause of morbidity and mortality worldwide in women. Basal-like breast cancer (BLBC) is associated with aggressive characteristics including development of recurrent disease and reduced survival. BLBC has been defined in some studies as tumours lacking both oestrogen receptor and progesterone receptor protein expression. Gene expression studies have shown that these tumours are also associated with expression of basal-type cytokeratins, the phenotypic patterns of basal cytokeratin expression in BLBC have not been widely studied. A well-characterised series of 995 invasive breast cancers with a long-term follow up were investigated using immunohistochemical staining for four basal cytokeratins (CK5, CK5/6, CK14 and CK17). The data were analysed using univariate and clustering analysis. As a result BLBC, as defined by negativity for ER and HER2 showed variable positivity for basal cytokeratin expression: 61.7 % CK5, 50.5 % CK5/6, 24.2 % CK14 and 23 % CK17. These characteristics were associated with poor outcome characteristics including high histological grade, mitosis, pleomorphism and tumour size >1.5 cm. CK5 positivity was more associated with ER(-), PgR(-), TN and double ER(-)PgR(-), than the other cytokeratins. Four different clusters of basal cytokeratin expression patterns were identified: (1) negativity for all basal cytokeratins, (2) CK5(+)/CK17(-), (3) CK5(-)/CK17(+) and (4) CK5(+)/CK17(+). These patterns of basal cytokeratin expression associated with differences in patient outcome, clusters 1 and 3 showed better outcomes than cluster 4 and 2, with cluster 2 having the poorest prognosis. In conclusion, four basal cytokeratin expression patterns were identified in human breast cancer using unsupervised clustering analysis and these patterns are associated with differences in patient outcome.

Authors+Show Affiliations

Department of Histopathology and School of Molecular Medical Sciences, The University of Nottingham and Nottingham University Hospitals NHS Trust, Nottingham City Hospital, Nottingham, UK. mrxaa31@nottingham.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23588953

Citation

Alshareeda, Alaa T., et al. "Characteristics of Basal Cytokeratin Expression in Breast Cancer." Breast Cancer Research and Treatment, vol. 139, no. 1, 2013, pp. 23-37.
Alshareeda AT, Soria D, Garibaldi JM, et al. Characteristics of basal cytokeratin expression in breast cancer. Breast Cancer Res Treat. 2013;139(1):23-37.
Alshareeda, A. T., Soria, D., Garibaldi, J. M., Rakha, E., Nolan, C., Ellis, I. O., & Green, A. R. (2013). Characteristics of basal cytokeratin expression in breast cancer. Breast Cancer Research and Treatment, 139(1), 23-37. https://doi.org/10.1007/s10549-013-2518-x
Alshareeda AT, et al. Characteristics of Basal Cytokeratin Expression in Breast Cancer. Breast Cancer Res Treat. 2013;139(1):23-37. PubMed PMID: 23588953.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of basal cytokeratin expression in breast cancer. AU - Alshareeda,Alaa T, AU - Soria,Daniele, AU - Garibaldi,Jonathan M, AU - Rakha,Emad, AU - Nolan,Christopher, AU - Ellis,Ian O, AU - Green,Andrew R, Y1 - 2013/04/16/ PY - 2013/03/31/received PY - 2013/04/01/accepted PY - 2013/4/17/entrez PY - 2013/4/17/pubmed PY - 2013/10/24/medline SP - 23 EP - 37 JF - Breast cancer research and treatment JO - Breast Cancer Res Treat VL - 139 IS - 1 N2 - Breast cancer is recognised to be a heterogeneous disease and the second most common cause of morbidity and mortality worldwide in women. Basal-like breast cancer (BLBC) is associated with aggressive characteristics including development of recurrent disease and reduced survival. BLBC has been defined in some studies as tumours lacking both oestrogen receptor and progesterone receptor protein expression. Gene expression studies have shown that these tumours are also associated with expression of basal-type cytokeratins, the phenotypic patterns of basal cytokeratin expression in BLBC have not been widely studied. A well-characterised series of 995 invasive breast cancers with a long-term follow up were investigated using immunohistochemical staining for four basal cytokeratins (CK5, CK5/6, CK14 and CK17). The data were analysed using univariate and clustering analysis. As a result BLBC, as defined by negativity for ER and HER2 showed variable positivity for basal cytokeratin expression: 61.7 % CK5, 50.5 % CK5/6, 24.2 % CK14 and 23 % CK17. These characteristics were associated with poor outcome characteristics including high histological grade, mitosis, pleomorphism and tumour size >1.5 cm. CK5 positivity was more associated with ER(-), PgR(-), TN and double ER(-)PgR(-), than the other cytokeratins. Four different clusters of basal cytokeratin expression patterns were identified: (1) negativity for all basal cytokeratins, (2) CK5(+)/CK17(-), (3) CK5(-)/CK17(+) and (4) CK5(+)/CK17(+). These patterns of basal cytokeratin expression associated with differences in patient outcome, clusters 1 and 3 showed better outcomes than cluster 4 and 2, with cluster 2 having the poorest prognosis. In conclusion, four basal cytokeratin expression patterns were identified in human breast cancer using unsupervised clustering analysis and these patterns are associated with differences in patient outcome. SN - 1573-7217 UR - https://www.unboundmedicine.com/medline/citation/23588953/Characteristics_of_basal_cytokeratin_expression_in_breast_cancer_ L2 - https://doi.org/10.1007/s10549-013-2518-x DB - PRIME DP - Unbound Medicine ER -