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Hair analysis for THCA-A, THC and CBN after passive in vivo exposure to marijuana smoke.
Drug Test Anal. 2014 Jan-Feb; 6(1-2):119-25.DT

Abstract

Condensation of marijuana smoke on the hair surface can be a source of an external contamination in hair analysis and may have serious consequences for the person under investigation. Δ9-tetrahydrocannabinolic acid A (THCA-A) is found in marijuana smoke and in hair analysis, but is not incorporated into the hair through the bloodstream. Therefore it might be a promising marker for external contamination of hair and could facilitate a more accurate interpretation of analytical results. In this study, three participants were exposed to the smoke of one joint every weekday over three weeks. Inhalation was excluded by an alternative breathing source. Hair samples were obtained up to seven weeks after the last exposure and analyzed for THCA-A, Δ9-tetrahydrocannabinol (THC) and cannabinol (CBN) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Additionally 30 hair samples from various regions of the head were obtained seven weeks after the exposure from one participant. The obtained results show that the degree of contamination depends on the hair length, with longer hair resulting in higher THC and CBN concentrations (1300 pg/mg and 530 pg/mg at the end of the exposure period) similar to the ones typically found after daily cannabis consumption. THCA-A could be detected in relatively low concentrations. Analysis of the distribution of the contamination showed that the posterior vertex region was affected most. The relatively low THCA-A concentrations in the samples suggest that most of the THCA-A found in forensic hair samples is not caused by sidestream marijuana smoke, but by other sources.

Authors+Show Affiliations

Institute of Forensic Medicine, Forensic Toxicology Department, University Medical Center Freiburg, Albertstr. 9, 79104 , Freiburg, Germany; Hermann Staudinger Graduate School, University of Freiburg, Hebelstraβe 27, 79104 , Freiburg, Germany.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23589391

Citation

Moosmann, Bjoern, et al. "Hair Analysis for THCA-A, THC and CBN After Passive in Vivo Exposure to Marijuana Smoke." Drug Testing and Analysis, vol. 6, no. 1-2, 2014, pp. 119-25.
Moosmann B, Roth N, Auwärter V. Hair analysis for THCA-A, THC and CBN after passive in vivo exposure to marijuana smoke. Drug Test Anal. 2014;6(1-2):119-25.
Moosmann, B., Roth, N., & Auwärter, V. (2014). Hair analysis for THCA-A, THC and CBN after passive in vivo exposure to marijuana smoke. Drug Testing and Analysis, 6(1-2), 119-25. https://doi.org/10.1002/dta.1474
Moosmann B, Roth N, Auwärter V. Hair Analysis for THCA-A, THC and CBN After Passive in Vivo Exposure to Marijuana Smoke. Drug Test Anal. 2014 Jan-Feb;6(1-2):119-25. PubMed PMID: 23589391.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hair analysis for THCA-A, THC and CBN after passive in vivo exposure to marijuana smoke. AU - Moosmann,Bjoern, AU - Roth,Nadine, AU - Auwärter,Volker, Y1 - 2013/04/15/ PY - 2013/01/21/received PY - 2013/02/22/revised PY - 2013/02/22/accepted PY - 2013/4/17/entrez PY - 2013/4/17/pubmed PY - 2014/9/16/medline KW - LC-MS/MS KW - contamination KW - hair analysis KW - tetrahydrocannabinol KW - tetrahydrocannabinolic acid A SP - 119 EP - 25 JF - Drug testing and analysis JO - Drug Test Anal VL - 6 IS - 1-2 N2 - Condensation of marijuana smoke on the hair surface can be a source of an external contamination in hair analysis and may have serious consequences for the person under investigation. Δ9-tetrahydrocannabinolic acid A (THCA-A) is found in marijuana smoke and in hair analysis, but is not incorporated into the hair through the bloodstream. Therefore it might be a promising marker for external contamination of hair and could facilitate a more accurate interpretation of analytical results. In this study, three participants were exposed to the smoke of one joint every weekday over three weeks. Inhalation was excluded by an alternative breathing source. Hair samples were obtained up to seven weeks after the last exposure and analyzed for THCA-A, Δ9-tetrahydrocannabinol (THC) and cannabinol (CBN) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Additionally 30 hair samples from various regions of the head were obtained seven weeks after the exposure from one participant. The obtained results show that the degree of contamination depends on the hair length, with longer hair resulting in higher THC and CBN concentrations (1300 pg/mg and 530 pg/mg at the end of the exposure period) similar to the ones typically found after daily cannabis consumption. THCA-A could be detected in relatively low concentrations. Analysis of the distribution of the contamination showed that the posterior vertex region was affected most. The relatively low THCA-A concentrations in the samples suggest that most of the THCA-A found in forensic hair samples is not caused by sidestream marijuana smoke, but by other sources. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/23589391/Hair_analysis_for_THCA_A_THC_and_CBN_after_passive_in_vivo_exposure_to_marijuana_smoke_ L2 - https://doi.org/10.1002/dta.1474 DB - PRIME DP - Unbound Medicine ER -