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Knockdown of both FoxO1 and C/EBPβ promotes adipogenesis in porcine preadipocytes through feedback regulation.
Cell Biol Int. 2013 Sep; 37(9):905-16.CB

Abstract

FoxO1 and C/EBPβ are important transcription factors during adipogenic differentiation; however, the potential mechanisms of their inter-regulation in adipogenesis remain unknown. We found that FoxO1 and C/EBPβ are abundant in adipose tissues of 3- and 180-day pigs at the mRNA and protein levels. During porcine preadipocyte differentiation, C/EBPβ expression increases to the peak at Day 2 and then decreases. In contrast, FoxO1 is lowest on Day 2 and gradually increases. Knockdown of FoxO1 or C/EBPβ with lentivirus-mediated shRNA enhances or inhibits lipid accumulation and adipogenic maker (C/EBPα and aP2) expression, respectively. FoxO1 depletion causes a mild decrease of C/EBPβ protein level, and C/EBPβ interference also inhibits the expression of FoxO1 protein. Knockdown of both FoxO1 and C/EBPβ promotes lipid accumulation at Day 8, and increases the adipogenic markers during differentiation in comparison with the controls and the FoxO1 knockdown alone. Co-immunoprecipitation experiments indicate that FoxO1 binds to C/EBPβ in adipose tissues and in vitro. In conclusion, the results suggest that FoxO1 and C/EBPβ regulate preadipocyte adipogenesis possibly through C/EBPβ → FoxO1 → C/EBPβ feedback regulatory loop and FoxO1-C/EBPβ protein complex.

Authors+Show Affiliations

Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A&F University, Yangling, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23589423

Citation

Yan, Xiong, et al. "Knockdown of Both FoxO1 and C/EBPβ Promotes Adipogenesis in Porcine Preadipocytes Through Feedback Regulation." Cell Biology International, vol. 37, no. 9, 2013, pp. 905-16.
Yan X, Weijun P, Ning W, et al. Knockdown of both FoxO1 and C/EBPβ promotes adipogenesis in porcine preadipocytes through feedback regulation. Cell Biol Int. 2013;37(9):905-16.
Yan, X., Weijun, P., Ning, W., Yu, W., Wenkai, R., & Gongshe, Y. (2013). Knockdown of both FoxO1 and C/EBPβ promotes adipogenesis in porcine preadipocytes through feedback regulation. Cell Biology International, 37(9), 905-16. https://doi.org/10.1002/cbin.10115
Yan X, et al. Knockdown of Both FoxO1 and C/EBPβ Promotes Adipogenesis in Porcine Preadipocytes Through Feedback Regulation. Cell Biol Int. 2013;37(9):905-16. PubMed PMID: 23589423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockdown of both FoxO1 and C/EBPβ promotes adipogenesis in porcine preadipocytes through feedback regulation. AU - Yan,Xiong, AU - Weijun,Pang, AU - Ning,Wei, AU - Yu,Wang, AU - Wenkai,Ren, AU - Gongshe,Yang, Y1 - 2013/05/07/ PY - 2012/11/20/received PY - 2013/03/22/accepted PY - 2013/4/17/entrez PY - 2013/4/17/pubmed PY - 2014/2/7/medline KW - C/EBPβ KW - FoxO1 KW - adipogenesis KW - pig KW - preadipocyte SP - 905 EP - 16 JF - Cell biology international JO - Cell Biol Int VL - 37 IS - 9 N2 - FoxO1 and C/EBPβ are important transcription factors during adipogenic differentiation; however, the potential mechanisms of their inter-regulation in adipogenesis remain unknown. We found that FoxO1 and C/EBPβ are abundant in adipose tissues of 3- and 180-day pigs at the mRNA and protein levels. During porcine preadipocyte differentiation, C/EBPβ expression increases to the peak at Day 2 and then decreases. In contrast, FoxO1 is lowest on Day 2 and gradually increases. Knockdown of FoxO1 or C/EBPβ with lentivirus-mediated shRNA enhances or inhibits lipid accumulation and adipogenic maker (C/EBPα and aP2) expression, respectively. FoxO1 depletion causes a mild decrease of C/EBPβ protein level, and C/EBPβ interference also inhibits the expression of FoxO1 protein. Knockdown of both FoxO1 and C/EBPβ promotes lipid accumulation at Day 8, and increases the adipogenic markers during differentiation in comparison with the controls and the FoxO1 knockdown alone. Co-immunoprecipitation experiments indicate that FoxO1 binds to C/EBPβ in adipose tissues and in vitro. In conclusion, the results suggest that FoxO1 and C/EBPβ regulate preadipocyte adipogenesis possibly through C/EBPβ → FoxO1 → C/EBPβ feedback regulatory loop and FoxO1-C/EBPβ protein complex. SN - 1095-8355 UR - https://www.unboundmedicine.com/medline/citation/23589423/Knockdown_of_both_FoxO1_and_C/EBPβ_promotes_adipogenesis_in_porcine_preadipocytes_through_feedback_regulation_ L2 - https://doi.org/10.1002/cbin.10115 DB - PRIME DP - Unbound Medicine ER -