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The human TREX-2 complex is stably associated with the nuclear pore basket.
J Cell Sci. 2013 Jun 15; 126(Pt 12):2656-67.JC

Abstract

In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NPC. The transcription and export complex 2 (TREX-2) was suggested to interact with NXF1 and to shuttle between transcription sites and the NPC. Here, we characterize the dynamics of human TREX-2 and show that it stably associates with the NPC basket. Moreover, the association of TREX-2 with the NPC requires the basket nucleoporins NUP153 and TPR, but is independent of transcription. Differential profiles of mRNA nuclear accumulation reveal that TREX-2 functions similarly to basket nucleoporins, but differently from NXF1. Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions.

Authors+Show Affiliations

Cellular Signaling and Nuclear Dynamics Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U964, Université de Strasbourg, BP 10142 - 67404 ILLKIRCH Cedex, CU de Strasbourg, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23591820

Citation

Umlauf, David, et al. "The Human TREX-2 Complex Is Stably Associated With the Nuclear Pore Basket." Journal of Cell Science, vol. 126, no. Pt 12, 2013, pp. 2656-67.
Umlauf D, Bonnet J, Waharte F, et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J Cell Sci. 2013;126(Pt 12):2656-67.
Umlauf, D., Bonnet, J., Waharte, F., Fournier, M., Stierle, M., Fischer, B., Brino, L., Devys, D., & Tora, L. (2013). The human TREX-2 complex is stably associated with the nuclear pore basket. Journal of Cell Science, 126(Pt 12), 2656-67. https://doi.org/10.1242/jcs.118000
Umlauf D, et al. The Human TREX-2 Complex Is Stably Associated With the Nuclear Pore Basket. J Cell Sci. 2013 Jun 15;126(Pt 12):2656-67. PubMed PMID: 23591820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The human TREX-2 complex is stably associated with the nuclear pore basket. AU - Umlauf,David, AU - Bonnet,Jacques, AU - Waharte,François, AU - Fournier,Marjorie, AU - Stierle,Matthieu, AU - Fischer,Benoit, AU - Brino,Laurent, AU - Devys,Didier, AU - Tora,László, Y1 - 2013/04/16/ PY - 2013/4/18/entrez PY - 2013/4/18/pubmed PY - 2014/2/28/medline KW - AMEX KW - NXF1 KW - Nuclear pore complex KW - THO/TREX KW - TREX-2 KW - Transcription KW - mRNA export SP - 2656 EP - 67 JF - Journal of cell science JO - J Cell Sci VL - 126 IS - Pt 12 N2 - In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NPC. The transcription and export complex 2 (TREX-2) was suggested to interact with NXF1 and to shuttle between transcription sites and the NPC. Here, we characterize the dynamics of human TREX-2 and show that it stably associates with the NPC basket. Moreover, the association of TREX-2 with the NPC requires the basket nucleoporins NUP153 and TPR, but is independent of transcription. Differential profiles of mRNA nuclear accumulation reveal that TREX-2 functions similarly to basket nucleoporins, but differently from NXF1. Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions. SN - 1477-9137 UR - https://www.unboundmedicine.com/medline/citation/23591820/The_human_TREX_2_complex_is_stably_associated_with_the_nuclear_pore_basket_ L2 - http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=23591820 DB - PRIME DP - Unbound Medicine ER -