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Molecular mechanisms of HMW glutenin subunits from 1S(l) genome of Aegilops longissima positively affecting wheat breadmaking quality.
PLoS One. 2013; 8(4):e58947.Plos

Abstract

A wheat cultivar "Chinese Spring" chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1S(l)×2.3* and 1S(l)y16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1S(l), making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat.

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Authors+Show Affiliations

Wang S
Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, China.
Yu Z
No affiliation info available
Cao M
No affiliation info available
Shen X
No affiliation info available
Li N
No affiliation info available
Li X
No affiliation info available
Ma W
No affiliation info available
Weiβgerber H
No affiliation info available
Zeller F
No affiliation info available
Hsam S
No affiliation info available
Yan Y
No affiliation info available

MeSH

BreadEdible GrainEndospermEvolution, MolecularFlourGene Expression ProfilingGene Expression RegulationGenome, PlantGlutensINDEL MutationMolecular WeightPhylogenyPlant ProteinsPlants, Genetically ModifiedPoaceaePolymorphism, Single NucleotideProtein SubunitsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTranscription, GeneticTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23593125

Citation

Wang, Shunli, et al. "Molecular Mechanisms of HMW Glutenin Subunits From 1S(l) Genome of Aegilops Longissima Positively Affecting Wheat Breadmaking Quality." PloS One, vol. 8, no. 4, 2013, pp. e58947.
Wang S, Yu Z, Cao M, et al. Molecular mechanisms of HMW glutenin subunits from 1S(l) genome of Aegilops longissima positively affecting wheat breadmaking quality. PLoS One. 2013;8(4):e58947.
Wang, S., Yu, Z., Cao, M., Shen, X., Li, N., Li, X., Ma, W., Weiβgerber, H., Zeller, F., Hsam, S., & Yan, Y. (2013). Molecular mechanisms of HMW glutenin subunits from 1S(l) genome of Aegilops longissima positively affecting wheat breadmaking quality. PloS One, 8(4), e58947. https://doi.org/10.1371/journal.pone.0058947
Wang S, et al. Molecular Mechanisms of HMW Glutenin Subunits From 1S(l) Genome of Aegilops Longissima Positively Affecting Wheat Breadmaking Quality. PLoS One. 2013;8(4):e58947. PubMed PMID: 23593125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular mechanisms of HMW glutenin subunits from 1S(l) genome of Aegilops longissima positively affecting wheat breadmaking quality. AU - Wang,Shunli, AU - Yu,Zitong, AU - Cao,Min, AU - Shen,Xixi, AU - Li,Ning, AU - Li,Xiaohui, AU - Ma,Wujun, AU - Weiβgerber,H, AU - Zeller,Friedrich, AU - Hsam,Sai, AU - Yan,Yueming, Y1 - 2013/04/04/ PY - 2012/06/24/received PY - 2013/02/11/accepted PY - 2013/4/18/entrez PY - 2013/4/18/pubmed PY - 2013/11/5/medline SP - e58947 EP - e58947 JF - PloS one JO - PLoS One VL - 8 IS - 4 N2 - A wheat cultivar "Chinese Spring" chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1S(l)×2.3* and 1S(l)y16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1S(l), making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23593125/Molecular_mechanisms_of_HMW_glutenin_subunits_from_1S_l__genome_of_Aegilops_longissima_positively_affecting_wheat_breadmaking_quality_ L2 - https://dx.plos.org/10.1371/journal.pone.0058947 DB - PRIME DP - Unbound Medicine ER -