Tags

Type your tag names separated by a space and hit enter

Broad spectrum of mimiviridae virophage allows its isolation using a mimivirus reporter.
PLoS One. 2013; 8(4):e61912.Plos

Abstract

The giant virus Mimiviridae family includes 3 groups of viruses: group A (includes Acanthamoeba polyphaga Mimivirus), group B (includes Moumouvirus) and group C (includes Megavirus chilensis). Virophages have been isolated with both group A Mimiviridae (the Mamavirus strain) and the related Cafeteria roenbergensis virus, and they have also been described by bioinformatic analysis of the Phycodnavirus. Here, we found that the first two strains of virophages isolated with group A Mimiviridae can multiply easily in groups B and C and play a role in gene transfer among these virus subgroups. To isolate new virophages and their Mimiviridae host in the environment, we used PCR to identify a sample with a virophage and a group C Mimiviridae that failed to grow on amoeba. Moreover, we showed that virophages reduce the pathogenic effect of Mimivirus (plaque formation), establishing its parasitic role on Mimivirus. We therefore developed a co-culture procedure using Acanthamoeba polyphaga and Mimivirus to recover the detected virophage and then sequenced the virophage's genome. We present this technique as a novel approach to isolating virophages. We demonstrated that the newly identified virophages replicate in the viral factories of all three groups of Mimiviridae, suggesting that the spectrum of virophages is not limited to their initial host.

Authors+Show Affiliations

URMITE, UM63, CNRS 7278, IRD 198, Inserm 1095, Aix Marseille Universite, Marseille, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23596530

Citation

Gaia, Morgan, et al. "Broad Spectrum of Mimiviridae Virophage Allows Its Isolation Using a Mimivirus Reporter." PloS One, vol. 8, no. 4, 2013, pp. e61912.
Gaia M, Pagnier I, Campocasso A, et al. Broad spectrum of mimiviridae virophage allows its isolation using a mimivirus reporter. PLoS ONE. 2013;8(4):e61912.
Gaia, M., Pagnier, I., Campocasso, A., Fournous, G., Raoult, D., & La Scola, B. (2013). Broad spectrum of mimiviridae virophage allows its isolation using a mimivirus reporter. PloS One, 8(4), e61912. https://doi.org/10.1371/journal.pone.0061912
Gaia M, et al. Broad Spectrum of Mimiviridae Virophage Allows Its Isolation Using a Mimivirus Reporter. PLoS ONE. 2013;8(4):e61912. PubMed PMID: 23596530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Broad spectrum of mimiviridae virophage allows its isolation using a mimivirus reporter. AU - Gaia,Morgan, AU - Pagnier,Isabelle, AU - Campocasso,Angélique, AU - Fournous,Ghislain, AU - Raoult,Didier, AU - La Scola,Bernard, Y1 - 2013/04/15/ PY - 2012/08/14/received PY - 2013/03/18/accepted PY - 2013/4/19/entrez PY - 2013/4/19/pubmed PY - 2013/10/23/medline SP - e61912 EP - e61912 JF - PloS one JO - PLoS ONE VL - 8 IS - 4 N2 - The giant virus Mimiviridae family includes 3 groups of viruses: group A (includes Acanthamoeba polyphaga Mimivirus), group B (includes Moumouvirus) and group C (includes Megavirus chilensis). Virophages have been isolated with both group A Mimiviridae (the Mamavirus strain) and the related Cafeteria roenbergensis virus, and they have also been described by bioinformatic analysis of the Phycodnavirus. Here, we found that the first two strains of virophages isolated with group A Mimiviridae can multiply easily in groups B and C and play a role in gene transfer among these virus subgroups. To isolate new virophages and their Mimiviridae host in the environment, we used PCR to identify a sample with a virophage and a group C Mimiviridae that failed to grow on amoeba. Moreover, we showed that virophages reduce the pathogenic effect of Mimivirus (plaque formation), establishing its parasitic role on Mimivirus. We therefore developed a co-culture procedure using Acanthamoeba polyphaga and Mimivirus to recover the detected virophage and then sequenced the virophage's genome. We present this technique as a novel approach to isolating virophages. We demonstrated that the newly identified virophages replicate in the viral factories of all three groups of Mimiviridae, suggesting that the spectrum of virophages is not limited to their initial host. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/23596530/Broad_spectrum_of_mimiviridae_virophage_allows_its_isolation_using_a_mimivirus_reporter_ L2 - http://dx.plos.org/10.1371/journal.pone.0061912 DB - PRIME DP - Unbound Medicine ER -