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Cooperative regulation of anxiety and panic-related defensive behaviors in the rat periaqueductal grey matter by 5-HT1A and μ-receptors.
J Psychopharmacol. 2013 Dec; 27(12):1141-8.JP

Abstract

Previous results with the elevated T-maze (ETM) test indicate that the antipanic action of serotonin (5-HT) in the dorsal periaqueductal grey (dPAG) depends on the activation endogenous opioid peptides. The aim of the present work was to investigate the interaction between opioid- and serotonin-mediated neurotransmission in the modulation of defensive responses in rats submitted to the ETM. The obtained results showed that intra-dPAG administration of morphine significantly increased escape latency, a panicolytic-like effect that was blocked by pre-treatment with intra-dPAG injection of either naloxone or the 5-HT1A antagonist N-[2-[4-(2-methoxyphenyl)-1 piperazinyl] ethyl] -N- 2- pyridinyl-ciclohexanecarboxamide maleate (WAY-100635). In addition, previous administration of naloxone antagonized both the anti-escape and the anti-avoidance (anxiolytic-like) effect of the 5-HT1A agonist (±)-8-hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8-OH-DPAT), but did not affect the anti-escape effect of the 5-HT2A agonist (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI). Moreover, the combination of sub-effective doses of locally administered 5-HT and morphine significantly impaired ETM escape performance. Finally, the µ-antagonist D-PHE-CYS-TYR-D-TRP-ORN-THR-PEN (CTOP) blocked the anti-avoidance as well as the anti-escape effect of 8-OHDPAT, and the association of sub-effective doses of the µ-opioid receptor agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-enkephalin acetate salt (DAMGO) and of 8-OHDPAT had anti-escape and anti-avoidance effects in the ETM. These results suggest a synergic interaction between the 5-HT1A and the µ-opioid receptor at post-synaptic level on neurons of the dPAG that regulate proximal defense, theoretically related to panic attacks.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Roncon CM
1Department of Pharmacology and Therapeutics, State University of Maringá, Brazil.
Biesdorf C
No affiliation info available
Coimbra NC
No affiliation info available
Audi EA
No affiliation info available
Zangrossi H
No affiliation info available
Graeff FG
No affiliation info available

MeSH

AnimalsAnxietyBehavior, AnimalMaleMaze LearningMorphineNaloxoneNeuronsPanicPanic DisorderPeriaqueductal GrayRatsRats, WistarReceptor, Serotonin, 5-HT1AReceptors, Opioid, muSerotoninSerotonin AntagonistsSerotonin Receptor Agonists

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23598399

Citation

Roncon, Camila M., et al. "Cooperative Regulation of Anxiety and Panic-related Defensive Behaviors in the Rat Periaqueductal Grey Matter By 5-HT1A and Μ-receptors." Journal of Psychopharmacology (Oxford, England), vol. 27, no. 12, 2013, pp. 1141-8.
Roncon CM, Biesdorf C, Coimbra NC, et al. Cooperative regulation of anxiety and panic-related defensive behaviors in the rat periaqueductal grey matter by 5-HT1A and μ-receptors. J Psychopharmacol. 2013;27(12):1141-8.
Roncon, C. M., Biesdorf, C., Coimbra, N. C., Audi, E. A., Zangrossi, H., & Graeff, F. G. (2013). Cooperative regulation of anxiety and panic-related defensive behaviors in the rat periaqueductal grey matter by 5-HT1A and μ-receptors. Journal of Psychopharmacology (Oxford, England), 27(12), 1141-8. https://doi.org/10.1177/0269881113485144
Roncon CM, et al. Cooperative Regulation of Anxiety and Panic-related Defensive Behaviors in the Rat Periaqueductal Grey Matter By 5-HT1A and Μ-receptors. J Psychopharmacol. 2013;27(12):1141-8. PubMed PMID: 23598399.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cooperative regulation of anxiety and panic-related defensive behaviors in the rat periaqueductal grey matter by 5-HT1A and μ-receptors. AU - Roncon,Camila M, AU - Biesdorf,Carla, AU - Coimbra,Norberto C, AU - Audi,Elisabeth A, AU - Zangrossi,Hélio,Jr AU - Graeff,Frederico G, Y1 - 2013/04/17/ PY - 2013/4/20/entrez PY - 2013/4/20/pubmed PY - 2015/1/28/medline KW - 5-HT1A receptor KW - Serotonin KW - elevated T-maze KW - endogenous opioid peptides KW - periaqueductal grey matter KW - µ-opioid receptor SP - 1141 EP - 8 JF - Journal of psychopharmacology (Oxford, England) JO - J Psychopharmacol VL - 27 IS - 12 N2 - Previous results with the elevated T-maze (ETM) test indicate that the antipanic action of serotonin (5-HT) in the dorsal periaqueductal grey (dPAG) depends on the activation endogenous opioid peptides. The aim of the present work was to investigate the interaction between opioid- and serotonin-mediated neurotransmission in the modulation of defensive responses in rats submitted to the ETM. The obtained results showed that intra-dPAG administration of morphine significantly increased escape latency, a panicolytic-like effect that was blocked by pre-treatment with intra-dPAG injection of either naloxone or the 5-HT1A antagonist N-[2-[4-(2-methoxyphenyl)-1 piperazinyl] ethyl] -N- 2- pyridinyl-ciclohexanecarboxamide maleate (WAY-100635). In addition, previous administration of naloxone antagonized both the anti-escape and the anti-avoidance (anxiolytic-like) effect of the 5-HT1A agonist (±)-8-hydroxy-2-(di-n-propylamino)tetralin hydrobromide (8-OH-DPAT), but did not affect the anti-escape effect of the 5-HT2A agonist (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI). Moreover, the combination of sub-effective doses of locally administered 5-HT and morphine significantly impaired ETM escape performance. Finally, the µ-antagonist D-PHE-CYS-TYR-D-TRP-ORN-THR-PEN (CTOP) blocked the anti-avoidance as well as the anti-escape effect of 8-OHDPAT, and the association of sub-effective doses of the µ-opioid receptor agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-enkephalin acetate salt (DAMGO) and of 8-OHDPAT had anti-escape and anti-avoidance effects in the ETM. These results suggest a synergic interaction between the 5-HT1A and the µ-opioid receptor at post-synaptic level on neurons of the dPAG that regulate proximal defense, theoretically related to panic attacks. SN - 1461-7285 UR - https://www.unboundmedicine.com/medline/citation/23598399/Cooperative_regulation_of_anxiety_and_panic_related_defensive_behaviors_in_the_rat_periaqueductal_grey_matter_by_5_HT1A_and_μ_receptors_ DB - PRIME DP - Unbound Medicine ER -