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Emulsified isoflurane protects rat heart in situ after regional ischemia and reperfusion.
Fundam Clin Pharmacol. 2014 Apr; 28(2):190-8.FC

Abstract

Volatile anesthetic postconditioning reduces myocardial infarct size against ischemia/reperfusion (I/R) injury. We tested the hypothesis that emulsified isoflurane (EIso) administrated after ischemia exerts cardioprotection in a rat model of myocardial I/R. Male SD rats underwent 30-min coronary occlusion followed by 3-h reperfusion except for sham rats. All vehicles were administrated intravenously at reperfusion onset for 30 min. In the first study, 56 rats were given saline (CON), 30% intralipid (IL) and 1, 2, 4, 8 or 16 mL/kg EIso for infarct size measurement. In a second study, 32 rats were randomized to four groups and administrated saline in sham (sham) and control (CON) groups, 30% intralipid in IL group and 2 mL/kg emulsified isoflurane in EIso group. Cardiomyocytic enzyme activity was determined. Myocardial mitochondria and cytosol were isolated to determine mitochondrial energy metabolism, cytochrome c release, mitochondrial membrane potential (ΔΨm) and opening of the mitochondrial permeability transition pore (mPTP). Morphologic changes in mitochondria were observed by transmission electron microscopy. Compared with CON and IL, 2, 4 and 8 mL/kg EIso limited infarct size (P < 0.01). Serum levels of cardiac enzyme leakage were reduced in EIso-treated hearts compared with CON (P < 0.01 or P < 0.05). EIso preserved the ultrastructure of mitochondria, protected against mPTP opening, decreased cytochrome c release and preserved ATP production and ΔΨm . In conclusion, EIso is effective in reducing infarct size and in preserving mitochondrial function after ischemia and reperfusion injury.

Authors+Show Affiliations

Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, 610041, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23600699

Citation

Hu, Zhao-Yang, et al. "Emulsified Isoflurane Protects Rat Heart in Situ After Regional Ischemia and Reperfusion." Fundamental & Clinical Pharmacology, vol. 28, no. 2, 2014, pp. 190-8.
Hu ZY, Peng XY, Liu F, et al. Emulsified isoflurane protects rat heart in situ after regional ischemia and reperfusion. Fundam Clin Pharmacol. 2014;28(2):190-8.
Hu, Z. Y., Peng, X. Y., Liu, F., & Liu, J. (2014). Emulsified isoflurane protects rat heart in situ after regional ischemia and reperfusion. Fundamental & Clinical Pharmacology, 28(2), 190-8. https://doi.org/10.1111/fcp.12030
Hu ZY, et al. Emulsified Isoflurane Protects Rat Heart in Situ After Regional Ischemia and Reperfusion. Fundam Clin Pharmacol. 2014;28(2):190-8. PubMed PMID: 23600699.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emulsified isoflurane protects rat heart in situ after regional ischemia and reperfusion. AU - Hu,Zhao-Yang, AU - Peng,Xia-Ying, AU - Liu,Fei, AU - Liu,Jin, Y1 - 2013/04/21/ PY - 2012/06/30/received PY - 2013/02/19/revised PY - 2013/03/07/accepted PY - 2013/4/23/entrez PY - 2013/4/23/pubmed PY - 2014/10/28/medline KW - emulsified isoflurane KW - ischemia/reperfusion injury KW - mitochondria SP - 190 EP - 8 JF - Fundamental & clinical pharmacology JO - Fundam Clin Pharmacol VL - 28 IS - 2 N2 - Volatile anesthetic postconditioning reduces myocardial infarct size against ischemia/reperfusion (I/R) injury. We tested the hypothesis that emulsified isoflurane (EIso) administrated after ischemia exerts cardioprotection in a rat model of myocardial I/R. Male SD rats underwent 30-min coronary occlusion followed by 3-h reperfusion except for sham rats. All vehicles were administrated intravenously at reperfusion onset for 30 min. In the first study, 56 rats were given saline (CON), 30% intralipid (IL) and 1, 2, 4, 8 or 16 mL/kg EIso for infarct size measurement. In a second study, 32 rats were randomized to four groups and administrated saline in sham (sham) and control (CON) groups, 30% intralipid in IL group and 2 mL/kg emulsified isoflurane in EIso group. Cardiomyocytic enzyme activity was determined. Myocardial mitochondria and cytosol were isolated to determine mitochondrial energy metabolism, cytochrome c release, mitochondrial membrane potential (ΔΨm) and opening of the mitochondrial permeability transition pore (mPTP). Morphologic changes in mitochondria were observed by transmission electron microscopy. Compared with CON and IL, 2, 4 and 8 mL/kg EIso limited infarct size (P < 0.01). Serum levels of cardiac enzyme leakage were reduced in EIso-treated hearts compared with CON (P < 0.01 or P < 0.05). EIso preserved the ultrastructure of mitochondria, protected against mPTP opening, decreased cytochrome c release and preserved ATP production and ΔΨm . In conclusion, EIso is effective in reducing infarct size and in preserving mitochondrial function after ischemia and reperfusion injury. SN - 1472-8206 UR - https://www.unboundmedicine.com/medline/citation/23600699/Emulsified_isoflurane_protects_rat_heart_in_situ_after_regional_ischemia_and_reperfusion_ L2 - https://doi.org/10.1111/fcp.12030 DB - PRIME DP - Unbound Medicine ER -