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Ultrasensitive electrochemical immunoassay for carcinoembryonic antigen based on three-dimensional macroporous gold nanoparticles/graphene composite platform and multienzyme functionalized nanoporous silver label.
Anal Chim Acta. 2013 May 02; 775:85-92.AC

Abstract

Three-dimensional macroporous gold nanoparticles/graphene composites (3D-AuNPs/GN) were synthesized through a simple two-step process, and were used to modify working electrode sensing platform, based on which a facile electrochemical immunoassay for sensitive detection of carcinoembryonic antigen (CEA) in human serum was developed. In the proposed 3D-AuNPs/GN, AuNPs were distributed not just on the surface, but also on the inside of graphene. And this distribution property increased the area of sensing surface, resulting in capturing more primary antibodies as well as improving the electronic transmission rate. In the presence of CEA, a sandwich-type immune composite was formed on the sensing platform, and the horseradish peroxidase-labeled anti-CEA antibody (HRP-Ab2)/thionine/nanoporous silver (HRP-Ab2/TH/NPS) signal label was captured. Under optimal conditions, the electrochemical immunosensor exhibited excellent analytical performance: the detection range of CEA is from 0.001 to 10 ng mL(-1) with low detection limit of 0.35 pg mL(-1) and low limit of quantitation (LOQ) of 0.85 pg mL(-1). The electrochemical immunosensor showed good precision, acceptable stability and reproducibility, and could be used for the detection of CEA in real samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules.

Authors+Show Affiliations

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23601978

Citation

Sun, Guoqiang, et al. "Ultrasensitive Electrochemical Immunoassay for Carcinoembryonic Antigen Based On Three-dimensional Macroporous Gold Nanoparticles/graphene Composite Platform and Multienzyme Functionalized Nanoporous Silver Label." Analytica Chimica Acta, vol. 775, 2013, pp. 85-92.
Sun G, Lu J, Ge S, et al. Ultrasensitive electrochemical immunoassay for carcinoembryonic antigen based on three-dimensional macroporous gold nanoparticles/graphene composite platform and multienzyme functionalized nanoporous silver label. Anal Chim Acta. 2013;775:85-92.
Sun, G., Lu, J., Ge, S., Song, X., Yu, J., Yan, M., & Huang, J. (2013). Ultrasensitive electrochemical immunoassay for carcinoembryonic antigen based on three-dimensional macroporous gold nanoparticles/graphene composite platform and multienzyme functionalized nanoporous silver label. Analytica Chimica Acta, 775, 85-92. https://doi.org/10.1016/j.aca.2013.03.009
Sun G, et al. Ultrasensitive Electrochemical Immunoassay for Carcinoembryonic Antigen Based On Three-dimensional Macroporous Gold Nanoparticles/graphene Composite Platform and Multienzyme Functionalized Nanoporous Silver Label. Anal Chim Acta. 2013 May 2;775:85-92. PubMed PMID: 23601978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive electrochemical immunoassay for carcinoembryonic antigen based on three-dimensional macroporous gold nanoparticles/graphene composite platform and multienzyme functionalized nanoporous silver label. AU - Sun,Guoqiang, AU - Lu,Juanjuan, AU - Ge,Shenguang, AU - Song,Xianrang, AU - Yu,Jinghua, AU - Yan,Mei, AU - Huang,Jiadong, Y1 - 2013/03/20/ PY - 2013/01/02/received PY - 2013/02/26/revised PY - 2013/03/03/accepted PY - 2013/4/23/entrez PY - 2013/4/23/pubmed PY - 2013/10/25/medline SP - 85 EP - 92 JF - Analytica chimica acta JO - Anal Chim Acta VL - 775 N2 - Three-dimensional macroporous gold nanoparticles/graphene composites (3D-AuNPs/GN) were synthesized through a simple two-step process, and were used to modify working electrode sensing platform, based on which a facile electrochemical immunoassay for sensitive detection of carcinoembryonic antigen (CEA) in human serum was developed. In the proposed 3D-AuNPs/GN, AuNPs were distributed not just on the surface, but also on the inside of graphene. And this distribution property increased the area of sensing surface, resulting in capturing more primary antibodies as well as improving the electronic transmission rate. In the presence of CEA, a sandwich-type immune composite was formed on the sensing platform, and the horseradish peroxidase-labeled anti-CEA antibody (HRP-Ab2)/thionine/nanoporous silver (HRP-Ab2/TH/NPS) signal label was captured. Under optimal conditions, the electrochemical immunosensor exhibited excellent analytical performance: the detection range of CEA is from 0.001 to 10 ng mL(-1) with low detection limit of 0.35 pg mL(-1) and low limit of quantitation (LOQ) of 0.85 pg mL(-1). The electrochemical immunosensor showed good precision, acceptable stability and reproducibility, and could be used for the detection of CEA in real samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/23601978/Ultrasensitive_electrochemical_immunoassay_for_carcinoembryonic_antigen_based_on_three_dimensional_macroporous_gold_nanoparticles/graphene_composite_platform_and_multienzyme_functionalized_nanoporous_silver_label_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(13)00325-5 DB - PRIME DP - Unbound Medicine ER -