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Rice Rab11 is required for JA-mediated defense signaling.
Biochem Biophys Res Commun. 2013 May 17; 434(4):797-802.BB

Abstract

Rab proteins play an essential role in regulating vesicular transport in eukaryotic cells. Previously, we characterized OsRab11, which in concert with OsGAP1 and OsGDI3 regulates vesicular trafficking from the trans-Golgi network (TGN) to the plasma membrane or vacuole. To further elucidate the physiological function of OsRab11 in plants, we performed yeast two-hybrid screens using OsRab11 as bait. OsOPR8 was isolated and shown to interact with OsRab11. A co-immunoprecipitation assay confirmed this interaction. The green fluorescent protein-OsOPR8 fusion product was targeted to the cytoplasm and peroxisomes of protoplasts from Arabidopsis thaliana. OsOPR8 exhibited NADPH-dependent reduction activity when 2-cyclohexen-1-one (CyHE) and 12-oxo-phytodienoic acid (OPDA) were supplied as possible substrates. Interestingly, NADPH oxidation by OsOPR8 was increased when wild-type OsRab11 or the constitutively active form of OsRab11 (Q78L) were included in the reaction mix, but not when the dominant negative form of OsRab11 (S28N) was included. OsRab11 was expressed broadly in plants and both OsRab11 and OsOPR8 were induced by jasmonic acid (JA) and elicitor treatments. Overexpressed OsRab11 transgenic plants showed resistance to pathogens through induced expression of JA-responsive genes. In conclusion, OsRab11 may be required for JA-mediated defense signaling by activating the reducing activity of OsOPR8.

Authors+Show Affiliations

Department of Molecular Biotechnology, Dong-A University, Busan 604-714, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23602899

Citation

Hong, Min Ji, et al. "Rice Rab11 Is Required for JA-mediated Defense Signaling." Biochemical and Biophysical Research Communications, vol. 434, no. 4, 2013, pp. 797-802.
Hong MJ, Lee Ym, Son YS, et al. Rice Rab11 is required for JA-mediated defense signaling. Biochem Biophys Res Commun. 2013;434(4):797-802.
Hong, M. J., Lee, Y. m., Son, Y. S., Im, C. H., Yi, Y. B., Rim, Y. G., Bahk, J. D., & Heo, J. B. (2013). Rice Rab11 is required for JA-mediated defense signaling. Biochemical and Biophysical Research Communications, 434(4), 797-802. https://doi.org/10.1016/j.bbrc.2013.04.014
Hong MJ, et al. Rice Rab11 Is Required for JA-mediated Defense Signaling. Biochem Biophys Res Commun. 2013 May 17;434(4):797-802. PubMed PMID: 23602899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rice Rab11 is required for JA-mediated defense signaling. AU - Hong,Min Ji, AU - Lee,Yun mi, AU - Son,Young Sim, AU - Im,Chak Han, AU - Yi,Young Byung, AU - Rim,Yeong Gil, AU - Bahk,Jeong Dong, AU - Heo,Jae Bok, Y1 - 2013/04/18/ PY - 2013/04/06/received PY - 2013/04/11/accepted PY - 2013/4/23/entrez PY - 2013/4/23/pubmed PY - 2013/8/28/medline SP - 797 EP - 802 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 434 IS - 4 N2 - Rab proteins play an essential role in regulating vesicular transport in eukaryotic cells. Previously, we characterized OsRab11, which in concert with OsGAP1 and OsGDI3 regulates vesicular trafficking from the trans-Golgi network (TGN) to the plasma membrane or vacuole. To further elucidate the physiological function of OsRab11 in plants, we performed yeast two-hybrid screens using OsRab11 as bait. OsOPR8 was isolated and shown to interact with OsRab11. A co-immunoprecipitation assay confirmed this interaction. The green fluorescent protein-OsOPR8 fusion product was targeted to the cytoplasm and peroxisomes of protoplasts from Arabidopsis thaliana. OsOPR8 exhibited NADPH-dependent reduction activity when 2-cyclohexen-1-one (CyHE) and 12-oxo-phytodienoic acid (OPDA) were supplied as possible substrates. Interestingly, NADPH oxidation by OsOPR8 was increased when wild-type OsRab11 or the constitutively active form of OsRab11 (Q78L) were included in the reaction mix, but not when the dominant negative form of OsRab11 (S28N) was included. OsRab11 was expressed broadly in plants and both OsRab11 and OsOPR8 were induced by jasmonic acid (JA) and elicitor treatments. Overexpressed OsRab11 transgenic plants showed resistance to pathogens through induced expression of JA-responsive genes. In conclusion, OsRab11 may be required for JA-mediated defense signaling by activating the reducing activity of OsOPR8. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/23602899/Rice_Rab11_is_required_for_JA_mediated_defense_signaling_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(13)00640-2 DB - PRIME DP - Unbound Medicine ER -