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Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPARα and SREBP-1.
Food Chem Toxicol 2013; 58:198-209FC

Abstract

We evaluated the effect of chicory (Cichorium intybus L.) seed extract (CI) on hepatic steatosis caused by early and late stage diabetes in rats (in vivo), and induced in HepG2 cells (in vitro) by BSA-oleic acid complex (OA). Different dosages of CI (1.25, 2.5 and 5 mg/ml) were applied along with OA (1 mM) to HepG2 cells, simultaneously and non-simultaneously; and without OA to ordinary non-steatotic cells. Cellular lipid accumulation and glycerol release, and hepatic triglyceride (TG) content were measured. The expression levels of sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferator-activated receptor alpha (PPARα) were determined. Liver samples were stained with hematoxylin and eosin (H&E). Significant histological damage (steatosis-inflammation-fibrosis) to the cells and tissues and down-regulation of SREBP-1c and PPARα genes that followed steatosis induction were prevented by CI in simultaneous treatment. In non-simultaneous treatment, CI up-regulated the expression of both genes and restored the normal levels of the corresponding proteins; with a greater stimulating effect on PPARα, CI acted as a PPARα agonist. CI released glycerol from HepG2 cells, and targeted the first and the second hit phases of hepatic steatosis. A preliminary attempt to characterize CI showed caffeic acid, chlorogenic acid, and chicoric acid, among the constituents.

Authors+Show Affiliations

Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23603006

Citation

Ziamajidi, Nasrin, et al. "Amelioration By Chicory Seed Extract of Diabetes- and Oleic Acid-induced Non-alcoholic Fatty Liver Disease (NAFLD)/non-alcoholic Steatohepatitis (NASH) Via Modulation of PPARα and SREBP-1." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 58, 2013, pp. 198-209.
Ziamajidi N, Khaghani S, Hassanzadeh G, et al. Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPARα and SREBP-1. Food Chem Toxicol. 2013;58:198-209.
Ziamajidi, N., Khaghani, S., Hassanzadeh, G., Vardasbi, S., Ahmadian, S., Nowrouzi, A., ... Abdirad, A. (2013). Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPARα and SREBP-1. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 58, pp. 198-209. doi:10.1016/j.fct.2013.04.018.
Ziamajidi N, et al. Amelioration By Chicory Seed Extract of Diabetes- and Oleic Acid-induced Non-alcoholic Fatty Liver Disease (NAFLD)/non-alcoholic Steatohepatitis (NASH) Via Modulation of PPARα and SREBP-1. Food Chem Toxicol. 2013;58:198-209. PubMed PMID: 23603006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amelioration by chicory seed extract of diabetes- and oleic acid-induced non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) via modulation of PPARα and SREBP-1. AU - Ziamajidi,Nasrin, AU - Khaghani,Shahnaz, AU - Hassanzadeh,Gholamreza, AU - Vardasbi,Safura, AU - Ahmadian,Shahram, AU - Nowrouzi,Azin, AU - Ghaffari,Seyed Mahmood, AU - Abdirad,Afshin, Y1 - 2013/04/18/ PY - 2012/10/18/received PY - 2013/03/30/revised PY - 2013/04/05/accepted PY - 2013/4/23/entrez PY - 2013/4/23/pubmed PY - 2014/3/7/medline KW - 0.1% Tween in phosphate buffer saline KW - 1,1-diphenyl-2-picryl hydrazyl radical KW - 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide KW - 50% inhibition concentration KW - ACAT KW - AGE KW - ALT KW - ANOVA KW - AST KW - BSA KW - BSA–oleic acid complex KW - BSDL KW - CI KW - CON KW - CYP7A1 KW - Chicory KW - Cichorium intybus (chicory) extract KW - DMEM KW - DMSO KW - DPPH KW - Diabetic rat KW - Dulbecco’s Modified Eagle’s Medium KW - ECL KW - FA KW - FBS KW - FFA KW - H&E KW - HCC KW - HL KW - HPLC KW - HRP KW - HepG2 KW - HepG2 cells KW - IC50 KW - LDH KW - MTT KW - NAFLD KW - NASH KW - NIA KW - OA KW - OD KW - ORO KW - Oil red O KW - PBST KW - PPARα KW - PTP1B KW - PVDF KW - Polyvinylidene fluoride KW - RIPA KW - ROS KW - SDS KW - SDS-PAGE KW - SDS-polyacrylamide gel KW - SREBP-1c KW - STZ KW - Steatosis KW - T2DM KW - TG KW - acyl-CoA:cholesterol acyltransferase KW - advanced glycation end-products KW - alanine aminotransferase KW - analysis of variance KW - aspartate aminotranferase KW - bile salt-dependent lipase KW - bovine serum albumin KW - cholesterol 7 alpha-hydroxylase, cholesterol 7-alpha-monooxygenase, or cytochrome P450 7A1 KW - control KW - dimethylsulfoxide KW - enhanced chemiluminescence KW - fatty acid KW - fetal bovine serum KW - free fatty acid KW - hematoxylin and eosin KW - hepatic lipase KW - hepatocellular carcinoma KW - high performance liquid chromatography KW - horseradish peroxidase KW - human hepatoma cell line KW - lactate dehydrogenase KW - niacinamide KW - non-alcoholic fatty liver disease KW - non-alcoholic steatohepatitis KW - optical density KW - peroxisome proliferator-activated receptor alpha KW - protein tyrosine phosphatase 1B KW - radioimmunoprecipitation assay buffer KW - reactive oxygen species KW - retention time KW - sodium dodecyl sulfate KW - sterol regulatory element-binding protein-1c KW - streptozotocin KW - t(R) KW - triglyceride KW - type 2 diabetes mellitus SP - 198 EP - 209 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 58 N2 - We evaluated the effect of chicory (Cichorium intybus L.) seed extract (CI) on hepatic steatosis caused by early and late stage diabetes in rats (in vivo), and induced in HepG2 cells (in vitro) by BSA-oleic acid complex (OA). Different dosages of CI (1.25, 2.5 and 5 mg/ml) were applied along with OA (1 mM) to HepG2 cells, simultaneously and non-simultaneously; and without OA to ordinary non-steatotic cells. Cellular lipid accumulation and glycerol release, and hepatic triglyceride (TG) content were measured. The expression levels of sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferator-activated receptor alpha (PPARα) were determined. Liver samples were stained with hematoxylin and eosin (H&E). Significant histological damage (steatosis-inflammation-fibrosis) to the cells and tissues and down-regulation of SREBP-1c and PPARα genes that followed steatosis induction were prevented by CI in simultaneous treatment. In non-simultaneous treatment, CI up-regulated the expression of both genes and restored the normal levels of the corresponding proteins; with a greater stimulating effect on PPARα, CI acted as a PPARα agonist. CI released glycerol from HepG2 cells, and targeted the first and the second hit phases of hepatic steatosis. A preliminary attempt to characterize CI showed caffeic acid, chlorogenic acid, and chicoric acid, among the constituents. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/23603006/Amelioration_by_chicory_seed_extract_of_diabetes__and_oleic_acid_induced_non_alcoholic_fatty_liver_disease__NAFLD_/non_alcoholic_steatohepatitis__NASH__via_modulation_of_PPARα_and_SREBP_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(13)00251-2 DB - PRIME DP - Unbound Medicine ER -