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Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus.
Antiviral Res. 2013 Jun; 98(3):449-56.AR

Abstract

We have shown previously that ultrafiltrates (5 kDa cutoff) of cell-free medium from mosquito cell cultures persistently infected with DENV serotype 2 (DENV-2) contained a novel antiviral agent (called viprolaxikine) that could protect pre-treated, naïve mosquito cells from DENV infection. Here, we show that viprolaxikine also reduced DENV-2 titers by almost 4 logs (>99.9%) when compared to Vero cells mock-treated with ultrafiltrates from cultures of uninfected mosquito cells. Protease treatment removed the anti-DENV-2 activity. Pre-incubation for 48-h was required to obtain the maximum, dose-dependent protection against DENV-2, indicating that the antiviral activity was based on the interaction between Vero cells and viprolaxikine rather than direct action of viprolaxikine on DENV-2. Activity was highest against DENV-2, but there was also significant activity against the 3 other DENV serotypes. LC-MS-MS analysis revealed that the active viprolaxikine fraction contained anionic, antiviral peptides, each comprised of 7 amino acids (DDHELQD, DETELQD and DEVMLQD or DEVLMQD) and with a common sequence motif of D-D/E-X-X-X-Q-D. These sequences do not occur in the dengue virus genome, suggesting that the peptides are produced by the host insect cells when persistently infected with DENV-2. These peptides represent a new class of anionic, insect-derived, antiviral peptides with activity against a flavivirus in both mammalian and insect cells.

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Authors+Show Affiliations

Laosutthipong C
Dept. Biotechnology, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
Kanthong N
No affiliation info available
Flegel TW
No affiliation info available

MeSH

Amino Acid MotifsAnimalsAnionsAntiviral AgentsChlorocebus aethiopsCulicidaeCytokinesDengueDengue VirusDose-Response Relationship, DrugHaplorhiniHost-Pathogen InteractionsInsect ProteinsPeptidesTime FactorsVero CellsViral Load

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23603496

Citation

Laosutthipong, Chaowanee, et al. "Novel, Anionic, Antiviral Septapeptides From Mosquito Cells Also Protect Monkey Cells Against Dengue Virus." Antiviral Research, vol. 98, no. 3, 2013, pp. 449-56.
Laosutthipong C, Kanthong N, Flegel TW. Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus. Antiviral Res. 2013;98(3):449-56.
Laosutthipong, C., Kanthong, N., & Flegel, T. W. (2013). Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus. Antiviral Research, 98(3), 449-56. https://doi.org/10.1016/j.antiviral.2013.04.011
Laosutthipong C, Kanthong N, Flegel TW. Novel, Anionic, Antiviral Septapeptides From Mosquito Cells Also Protect Monkey Cells Against Dengue Virus. Antiviral Res. 2013;98(3):449-56. PubMed PMID: 23603496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus. AU - Laosutthipong,Chaowanee, AU - Kanthong,Nipaporn, AU - Flegel,Timothy W, Y1 - 2013/04/17/ PY - 2013/01/18/received PY - 2013/04/10/revised PY - 2013/04/10/accepted PY - 2013/4/23/entrez PY - 2013/4/23/pubmed PY - 2013/12/24/medline SP - 449 EP - 56 JF - Antiviral research JO - Antiviral Res VL - 98 IS - 3 N2 - We have shown previously that ultrafiltrates (5 kDa cutoff) of cell-free medium from mosquito cell cultures persistently infected with DENV serotype 2 (DENV-2) contained a novel antiviral agent (called viprolaxikine) that could protect pre-treated, naïve mosquito cells from DENV infection. Here, we show that viprolaxikine also reduced DENV-2 titers by almost 4 logs (>99.9%) when compared to Vero cells mock-treated with ultrafiltrates from cultures of uninfected mosquito cells. Protease treatment removed the anti-DENV-2 activity. Pre-incubation for 48-h was required to obtain the maximum, dose-dependent protection against DENV-2, indicating that the antiviral activity was based on the interaction between Vero cells and viprolaxikine rather than direct action of viprolaxikine on DENV-2. Activity was highest against DENV-2, but there was also significant activity against the 3 other DENV serotypes. LC-MS-MS analysis revealed that the active viprolaxikine fraction contained anionic, antiviral peptides, each comprised of 7 amino acids (DDHELQD, DETELQD and DEVMLQD or DEVLMQD) and with a common sequence motif of D-D/E-X-X-X-Q-D. These sequences do not occur in the dengue virus genome, suggesting that the peptides are produced by the host insect cells when persistently infected with DENV-2. These peptides represent a new class of anionic, insect-derived, antiviral peptides with activity against a flavivirus in both mammalian and insect cells. SN - 1872-9096 UR - https://www.unboundmedicine.com/medline/citation/23603496/Novel_anionic_antiviral_septapeptides_from_mosquito_cells_also_protect_monkey_cells_against_dengue_virus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-3542(13)00097-1 DB - PRIME DP - Unbound Medicine ER -