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DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity.
Nat Cell Biol. 2013 May; 15(5):491-501.NC

Abstract

Organisms are constantly challenged by stresses and privations and require adaptive responses for their survival. The forkhead box O (FOXO) transcription factor DAF-16 (hereafter referred to as DAF-16/FOXO) is a central nexus in these responses, but despite its importance little is known about how it regulates its target genes. Proteomic identification of DAF-16/FOXO-binding partners in Caenorhabditis elegans and their subsequent functional evaluation by RNA interference revealed several candidate DAF-16/FOXO cofactors, most notably the chromatin remodeller SWI/SNF. DAF-16/FOXO and SWI/SNF form a complex and globally co-localize at DAF-16/FOXO target promoters. We show that specifically for gene activation, DAF-16/FOXO depends on SWI/SNF, facilitating SWI/SNF recruitment to target promoters, to activate transcription by presumed remodelling of local chromatin. For the animal, this translates into an essential role for SWI/SNF in DAF-16/FOXO-mediated processes, in particular dauer formation, stress resistance and the promotion of longevity. Thus, we give insight into the mechanisms of DAF-16/FOXO-mediated transcriptional regulation and establish a critical link between ATP-dependent chromatin remodelling and lifespan regulation.

Authors+Show Affiliations

Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23604319

Citation

Riedel, Christian G., et al. "DAF-16 Employs the Chromatin Remodeller SWI/SNF to Promote Stress Resistance and Longevity." Nature Cell Biology, vol. 15, no. 5, 2013, pp. 491-501.
Riedel CG, Dowen RH, Lourenco GF, et al. DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. Nat Cell Biol. 2013;15(5):491-501.
Riedel, C. G., Dowen, R. H., Lourenco, G. F., Kirienko, N. V., Heimbucher, T., West, J. A., Bowman, S. K., Kingston, R. E., Dillin, A., Asara, J. M., & Ruvkun, G. (2013). DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. Nature Cell Biology, 15(5), 491-501. https://doi.org/10.1038/ncb2720
Riedel CG, et al. DAF-16 Employs the Chromatin Remodeller SWI/SNF to Promote Stress Resistance and Longevity. Nat Cell Biol. 2013;15(5):491-501. PubMed PMID: 23604319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. AU - Riedel,Christian G, AU - Dowen,Robert H, AU - Lourenco,Guinevere F, AU - Kirienko,Natalia V, AU - Heimbucher,Thomas, AU - West,Jason A, AU - Bowman,Sarah K, AU - Kingston,Robert E, AU - Dillin,Andrew, AU - Asara,John M, AU - Ruvkun,Gary, Y1 - 2013/04/21/ PY - 2012/10/23/received PY - 2013/02/28/accepted PY - 2013/4/23/entrez PY - 2013/4/23/pubmed PY - 2013/6/26/medline SP - 491 EP - 501 JF - Nature cell biology JO - Nat. Cell Biol. VL - 15 IS - 5 N2 - Organisms are constantly challenged by stresses and privations and require adaptive responses for their survival. The forkhead box O (FOXO) transcription factor DAF-16 (hereafter referred to as DAF-16/FOXO) is a central nexus in these responses, but despite its importance little is known about how it regulates its target genes. Proteomic identification of DAF-16/FOXO-binding partners in Caenorhabditis elegans and their subsequent functional evaluation by RNA interference revealed several candidate DAF-16/FOXO cofactors, most notably the chromatin remodeller SWI/SNF. DAF-16/FOXO and SWI/SNF form a complex and globally co-localize at DAF-16/FOXO target promoters. We show that specifically for gene activation, DAF-16/FOXO depends on SWI/SNF, facilitating SWI/SNF recruitment to target promoters, to activate transcription by presumed remodelling of local chromatin. For the animal, this translates into an essential role for SWI/SNF in DAF-16/FOXO-mediated processes, in particular dauer formation, stress resistance and the promotion of longevity. Thus, we give insight into the mechanisms of DAF-16/FOXO-mediated transcriptional regulation and establish a critical link between ATP-dependent chromatin remodelling and lifespan regulation. SN - 1476-4679 UR - https://www.unboundmedicine.com/medline/citation/23604319/DAF_16_employs_the_chromatin_remodeller_SWI/SNF_to_promote_stress_resistance_and_longevity_ L2 - http://dx.doi.org/10.1038/ncb2720 DB - PRIME DP - Unbound Medicine ER -