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[Effect of montelukast on the expression of neurokinin-1 receptor in young asthmatic rats with airway remodeling].
Zhongguo Dang Dai Er Ke Za Zhi. 2013 Apr; 15(4):298-301.ZD

Abstract

OBJECTIVE

To investigate the effect of montelukast on the expression of sensory neuropeptide (neurokinin-1) receptor (NK1R) in young asthmatic rats with airway remodeling.

METHODS

Twenty-four Sprague-Dawley rats were randomly divided into control group (n=8), asthma (n=8), and montelukast groups (n=8). A rat model of asthma was induced by ovalbumin (OVA) inhalation. Normal saline was used instead of sensitizing solution and 1% OVA in the control group. Each rat in the montelukast group was given montelukast (15 mg/kg) by gavage 2 h before OVA inhalation. All rats received their respective treatments for 8 weeks. Immunohistochemistry, real-time PCR and Western blot were used to measure the mRNA and protein expression levels of NK1R in asthmatic airway remolding and to evaluate the effect of montelukast on NK1R expression.

RESULTS

The asthma group showed significantly higher mRNA and protein expression levels of NK1R than the control group (P<0.01). The mRNA and protein expression levels of NK1R in the montelukast group were significantly lower than in the asthma group (P<0.05), but significantly higher than in the control group (P<0.01).

CONCLUSIONS

Rats with induced asthma have upregulated NK1R expression in the airway, and montelukast can downregulate NK1R expression during airway remodeling.

Authors+Show Affiliations

Department of Pediatrics, 202 Military Hospital of People's Repablic of China, Shenyang, China. weib71@sina.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

23607955

Citation

Wei, Bing, et al. "[Effect of Montelukast On the Expression of Neurokinin-1 Receptor in Young Asthmatic Rats With Airway Remodeling]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 15, no. 4, 2013, pp. 298-301.
Wei B, Shang YX, Li M, et al. [Effect of montelukast on the expression of neurokinin-1 receptor in young asthmatic rats with airway remodeling]. Zhongguo Dang Dai Er Ke Za Zhi. 2013;15(4):298-301.
Wei, B., Shang, Y. X., Li, M., & Zhang, H. (2013). [Effect of montelukast on the expression of neurokinin-1 receptor in young asthmatic rats with airway remodeling]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 15(4), 298-301.
Wei B, et al. [Effect of Montelukast On the Expression of Neurokinin-1 Receptor in Young Asthmatic Rats With Airway Remodeling]. Zhongguo Dang Dai Er Ke Za Zhi. 2013;15(4):298-301. PubMed PMID: 23607955.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of montelukast on the expression of neurokinin-1 receptor in young asthmatic rats with airway remodeling]. AU - Wei,Bing, AU - Shang,Yun-Xiao, AU - Li,Miao, AU - Zhang,Han, PY - 2013/4/24/entrez PY - 2013/4/24/pubmed PY - 2013/8/9/medline SP - 298 EP - 301 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 15 IS - 4 N2 - OBJECTIVE: To investigate the effect of montelukast on the expression of sensory neuropeptide (neurokinin-1) receptor (NK1R) in young asthmatic rats with airway remodeling. METHODS: Twenty-four Sprague-Dawley rats were randomly divided into control group (n=8), asthma (n=8), and montelukast groups (n=8). A rat model of asthma was induced by ovalbumin (OVA) inhalation. Normal saline was used instead of sensitizing solution and 1% OVA in the control group. Each rat in the montelukast group was given montelukast (15 mg/kg) by gavage 2 h before OVA inhalation. All rats received their respective treatments for 8 weeks. Immunohistochemistry, real-time PCR and Western blot were used to measure the mRNA and protein expression levels of NK1R in asthmatic airway remolding and to evaluate the effect of montelukast on NK1R expression. RESULTS: The asthma group showed significantly higher mRNA and protein expression levels of NK1R than the control group (P<0.01). The mRNA and protein expression levels of NK1R in the montelukast group were significantly lower than in the asthma group (P<0.05), but significantly higher than in the control group (P<0.01). CONCLUSIONS: Rats with induced asthma have upregulated NK1R expression in the airway, and montelukast can downregulate NK1R expression during airway remodeling. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/23607955/[Effect_of_montelukast_on_the_expression_of_neurokinin_1_receptor_in_young_asthmatic_rats_with_airway_remodeling]_ L2 - http://www.zgddek.com/EN/abstract/abstract13050.shtml DB - PRIME DP - Unbound Medicine ER -