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Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats.
J Ethnopharmacol. 2013 Jun 21; 148(1):175-81.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

In traditional systems of medicine, stem bark of Acacia ferruginea DC. is used for the treatment of itching, leucoderma, ulcers, stomatitis and diseases of the blood. In the present study, we determined antioxidant and anti-ulcerogenic activities of Acacia ferruginea stem bark.

MATERIALS AND METHODS

Acetone extract and its sub-fractions of Acacia ferruginea stem bark were subjected to assess their antioxidant potential using various in vitro systems such as DPPH(•), ABTS(•+) scavenging, FRAP and phosphomolybdenum reduction activities. Based on the antioxidant potential, the ethyl acetate fraction was used to evaluate the protective effect of ethanol-induced gastric damage in rat model. Enzyme activities such as superoxide dismutase, glutathione, catalase and lipid peroxidation were also determined in the stomach tissues.

RESULTS

Ethyl acetate fraction (AFE) of Acacia ferruginea stem bark registered higher antioxidant and free radical scavenging activities than the crude acetone extract and other fractions. In addition, AFE exhibited that the IC50 values of DPPH (2.5µg/ml) and ABTS (1.8µg/ml) were lower when compared to the standard quercetin (12.4µg/ml and 4.7µg/ml, respectively). In ethanol induced gastric ulcer, administration of AFE at doses of 10mg/kg, 50mg/kg and 100mg/kg body weight prior to ethanol ingestion significantly protected the stomach ulceration. Consequently significant changes were observed in enzyme activities such as SOD, CAT, GSH and LPO in the stomach tissues when compared with ethanol control group.

CONCLUSION

It is concluded that the ethyl acetate fraction of Acacia ferruginea stem bark possessed higher antioxidant and anti-ulcerogenic activities. Based on the results, we suggest that Acacia ferruginea stem bark has potential to provide a therapeutic approach to ethanol mediated ulcer as an effective anti-ulcer agent.

Authors+Show Affiliations

Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk 712-714, Republic of Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23608239

Citation

Sowndhararajan, Kandhasamy, and Sun Chul Kang. "Protective Effect of Ethyl Acetate Fraction of Acacia Ferruginea DC. Against Ethanol-induced Gastric Ulcer in Rats." Journal of Ethnopharmacology, vol. 148, no. 1, 2013, pp. 175-81.
Sowndhararajan K, Kang SC. Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats. J Ethnopharmacol. 2013;148(1):175-81.
Sowndhararajan, K., & Kang, S. C. (2013). Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats. Journal of Ethnopharmacology, 148(1), 175-81. https://doi.org/10.1016/j.jep.2013.04.007
Sowndhararajan K, Kang SC. Protective Effect of Ethyl Acetate Fraction of Acacia Ferruginea DC. Against Ethanol-induced Gastric Ulcer in Rats. J Ethnopharmacol. 2013 Jun 21;148(1):175-81. PubMed PMID: 23608239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats. AU - Sowndhararajan,Kandhasamy, AU - Kang,Sun Chul, Y1 - 2013/04/19/ PY - 2012/12/28/received PY - 2013/03/01/revised PY - 2013/04/06/accepted PY - 2013/4/24/entrez PY - 2013/4/24/pubmed PY - 2014/1/8/medline SP - 175 EP - 81 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 148 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: In traditional systems of medicine, stem bark of Acacia ferruginea DC. is used for the treatment of itching, leucoderma, ulcers, stomatitis and diseases of the blood. In the present study, we determined antioxidant and anti-ulcerogenic activities of Acacia ferruginea stem bark. MATERIALS AND METHODS: Acetone extract and its sub-fractions of Acacia ferruginea stem bark were subjected to assess their antioxidant potential using various in vitro systems such as DPPH(•), ABTS(•+) scavenging, FRAP and phosphomolybdenum reduction activities. Based on the antioxidant potential, the ethyl acetate fraction was used to evaluate the protective effect of ethanol-induced gastric damage in rat model. Enzyme activities such as superoxide dismutase, glutathione, catalase and lipid peroxidation were also determined in the stomach tissues. RESULTS: Ethyl acetate fraction (AFE) of Acacia ferruginea stem bark registered higher antioxidant and free radical scavenging activities than the crude acetone extract and other fractions. In addition, AFE exhibited that the IC50 values of DPPH (2.5µg/ml) and ABTS (1.8µg/ml) were lower when compared to the standard quercetin (12.4µg/ml and 4.7µg/ml, respectively). In ethanol induced gastric ulcer, administration of AFE at doses of 10mg/kg, 50mg/kg and 100mg/kg body weight prior to ethanol ingestion significantly protected the stomach ulceration. Consequently significant changes were observed in enzyme activities such as SOD, CAT, GSH and LPO in the stomach tissues when compared with ethanol control group. CONCLUSION: It is concluded that the ethyl acetate fraction of Acacia ferruginea stem bark possessed higher antioxidant and anti-ulcerogenic activities. Based on the results, we suggest that Acacia ferruginea stem bark has potential to provide a therapeutic approach to ethanol mediated ulcer as an effective anti-ulcer agent. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/23608239/Protective_effect_of_ethyl_acetate_fraction_of_Acacia_ferruginea_DC__against_ethanol_induced_gastric_ulcer_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(13)00255-9 DB - PRIME DP - Unbound Medicine ER -