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Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae).
Ecol Evol. 2013 Apr; 3(4):918-33.EE

Abstract

Although medicinal leeches have long been used as treatment for various ailments because of their potent anticoagulation factors, neither the full diversity of salivary components that inhibit coagulation, nor the evolutionary selection acting on them has been thoroughly investigated. Here, we constructed expressed sequence tag libraries from salivary glands of two species of medicinal hirudinoid leeches, Hirudo verbana and Aliolimnatis fenestrata, and identified anticoagulant-orthologs through BLASTx searches. The data set then was augmented by the addition of a previously constructed EST library from the macrobdelloid leech Macrobdella decora. The identified orthologs then were compared and contrasted with well-characterized anticoagulants from a variety of leeches with different feeding habits, including non-sanguivorous species. Moreover, four different statistical methods for predicting signatures of positive and negative evolutionary pressures were used for 10 rounds each to assess the level and type of selection acting on the molecules as a whole and on specific sites. In total, sequences showing putative BLASTx-orthology with five and three anticoagulant-families were recovered in the A. fenestrata and H. verbana EST libraries respectively. Selection pressure analyses predicted high levels of purifying selection across the anticoagulant diversity, although a few isolated sites showed signatures of positive selection. This study represents a first attempt at mapping the anticoagulant repertoires in a comparative fashion across several leech families.

Authors+Show Affiliations

Richard Gilder Graduate School, American Museum of Natural History Central Park West at 79th Street, New York, NY 10024, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23610634

Citation

Kvist, Sebastian, et al. "Diversity and Selective Pressures of Anticoagulants in Three Medicinal Leeches (Hirudinida: Hirudinidae, Macrobdellidae)." Ecology and Evolution, vol. 3, no. 4, 2013, pp. 918-33.
Kvist S, Min GS, Siddall ME. Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae). Ecol Evol. 2013;3(4):918-33.
Kvist, S., Min, G. S., & Siddall, M. E. (2013). Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae). Ecology and Evolution, 3(4), 918-33. https://doi.org/10.1002/ece3.480
Kvist S, Min GS, Siddall ME. Diversity and Selective Pressures of Anticoagulants in Three Medicinal Leeches (Hirudinida: Hirudinidae, Macrobdellidae). Ecol Evol. 2013;3(4):918-33. PubMed PMID: 23610634.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and selective pressures of anticoagulants in three medicinal leeches (Hirudinida: Hirudinidae, Macrobdellidae). AU - Kvist,Sebastian, AU - Min,Gi-Sik, AU - Siddall,Mark E, Y1 - 2013/03/05/ PY - 2012/11/27/received PY - 2012/12/13/revised PY - 2012/12/18/accepted PY - 2013/4/24/entrez PY - 2013/4/24/pubmed PY - 2013/4/24/medline KW - Anticoagulant KW - FEL KW - Hirudinida KW - PARRIS KW - REL KW - SLAC KW - evolution KW - medicinal leeches KW - selection pressures SP - 918 EP - 33 JF - Ecology and evolution JO - Ecol Evol VL - 3 IS - 4 N2 - Although medicinal leeches have long been used as treatment for various ailments because of their potent anticoagulation factors, neither the full diversity of salivary components that inhibit coagulation, nor the evolutionary selection acting on them has been thoroughly investigated. Here, we constructed expressed sequence tag libraries from salivary glands of two species of medicinal hirudinoid leeches, Hirudo verbana and Aliolimnatis fenestrata, and identified anticoagulant-orthologs through BLASTx searches. The data set then was augmented by the addition of a previously constructed EST library from the macrobdelloid leech Macrobdella decora. The identified orthologs then were compared and contrasted with well-characterized anticoagulants from a variety of leeches with different feeding habits, including non-sanguivorous species. Moreover, four different statistical methods for predicting signatures of positive and negative evolutionary pressures were used for 10 rounds each to assess the level and type of selection acting on the molecules as a whole and on specific sites. In total, sequences showing putative BLASTx-orthology with five and three anticoagulant-families were recovered in the A. fenestrata and H. verbana EST libraries respectively. Selection pressure analyses predicted high levels of purifying selection across the anticoagulant diversity, although a few isolated sites showed signatures of positive selection. This study represents a first attempt at mapping the anticoagulant repertoires in a comparative fashion across several leech families. SN - 2045-7758 UR - https://www.unboundmedicine.com/medline/citation/23610634/Diversity_and_selective_pressures_of_anticoagulants_in_three_medicinal_leeches__Hirudinida:_Hirudinidae_Macrobdellidae__ L2 - https://doi.org/10.1002/ece3.480 DB - PRIME DP - Unbound Medicine ER -
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