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Effect of three drugs against Encephalitozoon cuniculi infection in immunosuppressed mice.
Antimicrob Agents Chemother. 2013 Jul; 57(7):3067-71.AA

Abstract

Microsporidia comprise a large group of obligate intracellular parasites. The microsporidian Encephalitozoon cuniculi causes disseminated infection in immunosuppressed patients with HIV, cancer, or transplants and in the elderly. In vivo and in vitro studies on the effectiveness of drugs are controversial. Currently, there is no effective treatment. We tested albendazole, albendazole sulfoxide, metronidazole, and cyclosporine in mice immunosuppressed with cyclophosphamide and inoculated by the intraperitoneal route with 10(7) E. cuniculi spores. One week after experimental inoculation, the mice were treated with albendazole, albendazole sulfoxide, metronidazole, and cyclosporine. Histological and morphometric analyses were performed to compare the treated groups. The state of immunosuppression was evaluated by phenotyping CD4(+) and CD8(+) T cells by flow cytometry. Nontreated mice showed acute disseminated and fatal encephalitozoonosis. The treatment with benzimidazoles significantly reduced infection until 30 days posttreatment (p.t.), but at 60 days p.t., the infection had recurred. Metronidazole decreased infection by a short time, and cyclosporine was not effective. All animals were immunosuppressed by all the experiments, as demonstrated by the low number of CD4(+) and CD8(+) T cells. We conclude that no drug was effective against E. cuniculi, but the benzimidazoles controlled the infection transiently.

Authors+Show Affiliations

Environmental and Experimental Pathology, Paulista University (UNIP), São Paulo, Brazil. maria.lallo@cruzeirodosul.edu.brNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

23612191

Citation

Lallo, Maria Anete, et al. "Effect of Three Drugs Against Encephalitozoon Cuniculi Infection in Immunosuppressed Mice." Antimicrobial Agents and Chemotherapy, vol. 57, no. 7, 2013, pp. 3067-71.
Lallo MA, da Costa LF, de Castro JM. Effect of three drugs against Encephalitozoon cuniculi infection in immunosuppressed mice. Antimicrob Agents Chemother. 2013;57(7):3067-71.
Lallo, M. A., da Costa, L. F., & de Castro, J. M. (2013). Effect of three drugs against Encephalitozoon cuniculi infection in immunosuppressed mice. Antimicrobial Agents and Chemotherapy, 57(7), 3067-71. https://doi.org/10.1128/AAC.00157-13
Lallo MA, da Costa LF, de Castro JM. Effect of Three Drugs Against Encephalitozoon Cuniculi Infection in Immunosuppressed Mice. Antimicrob Agents Chemother. 2013;57(7):3067-71. PubMed PMID: 23612191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of three drugs against Encephalitozoon cuniculi infection in immunosuppressed mice. AU - Lallo,Maria Anete, AU - da Costa,Lidiana F Vidoto, AU - de Castro,João Manoel, Y1 - 2013/04/22/ PY - 2013/4/25/entrez PY - 2013/4/25/pubmed PY - 2014/1/1/medline SP - 3067 EP - 71 JF - Antimicrobial agents and chemotherapy JO - Antimicrob. Agents Chemother. VL - 57 IS - 7 N2 - Microsporidia comprise a large group of obligate intracellular parasites. The microsporidian Encephalitozoon cuniculi causes disseminated infection in immunosuppressed patients with HIV, cancer, or transplants and in the elderly. In vivo and in vitro studies on the effectiveness of drugs are controversial. Currently, there is no effective treatment. We tested albendazole, albendazole sulfoxide, metronidazole, and cyclosporine in mice immunosuppressed with cyclophosphamide and inoculated by the intraperitoneal route with 10(7) E. cuniculi spores. One week after experimental inoculation, the mice were treated with albendazole, albendazole sulfoxide, metronidazole, and cyclosporine. Histological and morphometric analyses were performed to compare the treated groups. The state of immunosuppression was evaluated by phenotyping CD4(+) and CD8(+) T cells by flow cytometry. Nontreated mice showed acute disseminated and fatal encephalitozoonosis. The treatment with benzimidazoles significantly reduced infection until 30 days posttreatment (p.t.), but at 60 days p.t., the infection had recurred. Metronidazole decreased infection by a short time, and cyclosporine was not effective. All animals were immunosuppressed by all the experiments, as demonstrated by the low number of CD4(+) and CD8(+) T cells. We conclude that no drug was effective against E. cuniculi, but the benzimidazoles controlled the infection transiently. SN - 1098-6596 UR - https://www.unboundmedicine.com/medline/citation/23612191/Effect_of_three_drugs_against_Encephalitozoon_cuniculi_infection_in_immunosuppressed_mice_ L2 - http://aac.asm.org/cgi/pmidlookup?view=long&pmid=23612191 DB - PRIME DP - Unbound Medicine ER -