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Effects of Pb(Ⅱ) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis-related gene transcription.
Environ Toxicol. 2014 Nov; 29(11):1346-54.ET

Abstract

Greater exposure to Pb(Ⅱ) increases the likelihood of harmful effects in the environment. In this study, the aquatic unicellular alga Chlorella protothecoides (C. protothecoides) and Chlorella vulgaris (C. vulgaris) were chosen to assess the acute and chronic toxicity of Pb(Ⅱ) exposure. Results of the observations show dose-response relationships could be clearly observed between Pb(Ⅱ) concentration and percentage inhibition (PI). Exposure to Pb(Ⅱ) increased malondialdehyde (MDA) content by up to 4.22 times compared with the control, suggesting that there was some oxidative damage. ANOVA analysis shows that Pb(Ⅱ) decreased chlorophyll (chl) content, indicating marked concentration-dependent relationships, and the lowest levels of chl a, chl b, and total-chl were 14.53, 18.80, and 17.95% of the controls, respectively. A real-time PCR assay suggests the changes in transcript abundances of three photosynthetic-related genes. After 120 h exposure Pb(Ⅱ) reduced the transcript abundance of rbcL, psaB, and psbC, and the relative abundances of the three genes of C. protothecoides and C. vulgaris in response to Pb(Ⅱ) were 54.66-98.59, 51.68-95.59, 37.89-95.48, 36.04-94.94, 41.19-91.20, and 58.75-96.80% of those of the controls, respectively. As for 28 d treatments, the three genes displayed similar inhibitory trend. This research provides a basic understanding of Pb(Ⅱ) toxicity to aquatic organisms.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xiong B
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai, 200237, People's Republic of China; School of Resource and Environmental Engineering, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Zhang W
No affiliation info available
Chen L
No affiliation info available
Lin KF
No affiliation info available
Guo MJ
No affiliation info available
Wang WL
No affiliation info available
Cui XH
No affiliation info available
Bi HS
No affiliation info available
Wang B
No affiliation info available

MeSH

ChlorellaChlorella vulgarisChlorophyllChlorophyll AEnvironmental PollutantsLeadMalondialdehydeOxidation-ReductionPhotosynthesisSpecies SpecificityTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23613127

Citation

Xiong, Bang, et al. "Effects of Pb(Ⅱ) Exposure On Chlorella Protothecoides and Chlorella Vulgaris Growth, Malondialdehyde, and Photosynthesis-related Gene Transcription." Environmental Toxicology, vol. 29, no. 11, 2014, pp. 1346-54.
Xiong B, Zhang W, Chen L, et al. Effects of Pb(Ⅱ) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis-related gene transcription. Environ Toxicol. 2014;29(11):1346-54.
Xiong, B., Zhang, W., Chen, L., Lin, K. F., Guo, M. J., Wang, W. L., Cui, X. H., Bi, H. S., & Wang, B. (2014). Effects of Pb(Ⅱ) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis-related gene transcription. Environmental Toxicology, 29(11), 1346-54. https://doi.org/10.1002/tox.21865
Xiong B, et al. Effects of Pb(Ⅱ) Exposure On Chlorella Protothecoides and Chlorella Vulgaris Growth, Malondialdehyde, and Photosynthesis-related Gene Transcription. Environ Toxicol. 2014;29(11):1346-54. PubMed PMID: 23613127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of Pb(Ⅱ) exposure on Chlorella protothecoides and Chlorella vulgaris growth, malondialdehyde, and photosynthesis-related gene transcription. AU - Xiong,Bang, AU - Zhang,Wei, AU - Chen,Lin, AU - Lin,Kuang-Fei, AU - Guo,Mei-Jin, AU - Wang,Wei-Liang, AU - Cui,Xin-Hong, AU - Bi,Hua-Song, AU - Wang,Bin, Y1 - 2013/04/24/ PY - 2012/09/28/received PY - 2013/03/07/revised PY - 2013/03/16/accepted PY - 2013/4/25/entrez PY - 2013/4/25/pubmed PY - 2015/4/8/medline KW - Chlorella protothecoides KW - Chlorella vulgaris KW - Pb(Ⅱ) KW - gene transcription KW - physiological level SP - 1346 EP - 54 JF - Environmental toxicology JO - Environ Toxicol VL - 29 IS - 11 N2 - Greater exposure to Pb(Ⅱ) increases the likelihood of harmful effects in the environment. In this study, the aquatic unicellular alga Chlorella protothecoides (C. protothecoides) and Chlorella vulgaris (C. vulgaris) were chosen to assess the acute and chronic toxicity of Pb(Ⅱ) exposure. Results of the observations show dose-response relationships could be clearly observed between Pb(Ⅱ) concentration and percentage inhibition (PI). Exposure to Pb(Ⅱ) increased malondialdehyde (MDA) content by up to 4.22 times compared with the control, suggesting that there was some oxidative damage. ANOVA analysis shows that Pb(Ⅱ) decreased chlorophyll (chl) content, indicating marked concentration-dependent relationships, and the lowest levels of chl a, chl b, and total-chl were 14.53, 18.80, and 17.95% of the controls, respectively. A real-time PCR assay suggests the changes in transcript abundances of three photosynthetic-related genes. After 120 h exposure Pb(Ⅱ) reduced the transcript abundance of rbcL, psaB, and psbC, and the relative abundances of the three genes of C. protothecoides and C. vulgaris in response to Pb(Ⅱ) were 54.66-98.59, 51.68-95.59, 37.89-95.48, 36.04-94.94, 41.19-91.20, and 58.75-96.80% of those of the controls, respectively. As for 28 d treatments, the three genes displayed similar inhibitory trend. This research provides a basic understanding of Pb(Ⅱ) toxicity to aquatic organisms. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/23613127/Effects_of_Pb_Ⅱ__exposure_on_Chlorella_protothecoides_and_Chlorella_vulgaris_growth_malondialdehyde_and_photosynthesis_related_gene_transcription_ L2 - https://doi.org/10.1002/tox.21865 DB - PRIME DP - Unbound Medicine ER -