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Electrochemistry of cholesterol biosensor based on a novel Pt-Pd bimetallic nanoparticle decorated graphene catalyst.
Talanta. 2013 May 15; 109:167-72.T

Abstract

A new electrochemical biosensor with enhanced sensitivity was developed for detection of cholesterol by using platinum-palladium-chitosan-graphene hybrid nanocomposites (PtPd-CS-GS) functionalized glassy carbon electrode (GCE). An electrodeposition method was applied to form PtPd nanoparticles-doped chitosan-graphene hybrid nanocomposites (PtPd-CS-GS), which were characterized by scanning electron microscopy (SEM) and electrochemical methods. The presence of the PtPd-CS-GS nanocomposites not only accelerated direct electron transfer from the redox enzyme to the electrode surface, but also enhanced the immobilized amount of cholesterol oxidase (ChOx). Under optimal conditions, the fabricated biosensor exhibited wide linear ranges of responses to cholesterol in the concentration ranges of 2.2×10(-6) to 5.2×10(-4)M, the limit of detection was 0.75 μM (S/N=3). The response time was less than 7s and the Michaelis-Menten constant (Km(app)) was found as 0.11 mM. In addition, the biosensor also exhibited excellent reproducibility and stability. Along with these attractive features, the biosensor also displayed very high specificity to cholesterol with complete elimination of interference from UA, AA, and glucose.

Authors+Show Affiliations

Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23618155

Citation

Cao, Shurui, et al. "Electrochemistry of Cholesterol Biosensor Based On a Novel Pt-Pd Bimetallic Nanoparticle Decorated Graphene Catalyst." Talanta, vol. 109, 2013, pp. 167-72.
Cao S, Zhang L, Chai Y, et al. Electrochemistry of cholesterol biosensor based on a novel Pt-Pd bimetallic nanoparticle decorated graphene catalyst. Talanta. 2013;109:167-72.
Cao, S., Zhang, L., Chai, Y., & Yuan, R. (2013). Electrochemistry of cholesterol biosensor based on a novel Pt-Pd bimetallic nanoparticle decorated graphene catalyst. Talanta, 109, 167-72. https://doi.org/10.1016/j.talanta.2013.02.002
Cao S, et al. Electrochemistry of Cholesterol Biosensor Based On a Novel Pt-Pd Bimetallic Nanoparticle Decorated Graphene Catalyst. Talanta. 2013 May 15;109:167-72. PubMed PMID: 23618155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemistry of cholesterol biosensor based on a novel Pt-Pd bimetallic nanoparticle decorated graphene catalyst. AU - Cao,Shurui, AU - Zhang,Lei, AU - Chai,Yaqin, AU - Yuan,Ruo, Y1 - 2013/02/18/ PY - 2012/10/17/received PY - 2013/01/30/revised PY - 2013/02/01/accepted PY - 2013/4/27/entrez PY - 2013/4/27/pubmed PY - 2013/11/13/medline SP - 167 EP - 72 JF - Talanta JO - Talanta VL - 109 N2 - A new electrochemical biosensor with enhanced sensitivity was developed for detection of cholesterol by using platinum-palladium-chitosan-graphene hybrid nanocomposites (PtPd-CS-GS) functionalized glassy carbon electrode (GCE). An electrodeposition method was applied to form PtPd nanoparticles-doped chitosan-graphene hybrid nanocomposites (PtPd-CS-GS), which were characterized by scanning electron microscopy (SEM) and electrochemical methods. The presence of the PtPd-CS-GS nanocomposites not only accelerated direct electron transfer from the redox enzyme to the electrode surface, but also enhanced the immobilized amount of cholesterol oxidase (ChOx). Under optimal conditions, the fabricated biosensor exhibited wide linear ranges of responses to cholesterol in the concentration ranges of 2.2×10(-6) to 5.2×10(-4)M, the limit of detection was 0.75 μM (S/N=3). The response time was less than 7s and the Michaelis-Menten constant (Km(app)) was found as 0.11 mM. In addition, the biosensor also exhibited excellent reproducibility and stability. Along with these attractive features, the biosensor also displayed very high specificity to cholesterol with complete elimination of interference from UA, AA, and glucose. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/23618155/Electrochemistry_of_cholesterol_biosensor_based_on_a_novel_Pt_Pd_bimetallic_nanoparticle_decorated_graphene_catalyst_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(13)00075-1 DB - PRIME DP - Unbound Medicine ER -