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Determination of ephedrine and pseudoephedrine by field-amplified sample injection capillary electrophoresis.
J Chromatogr Sci. 2014 Apr; 52(4):357-62.JC

Abstract

A simple and rapid capillary electrophoresis method was developed for the separation and determination of ephedrine (E) and pseudoephedrine (PE) in a buffer solution containing 80 mM of NaH2PO4 (pH 3.0), 15 mM of β-cyclodextrin and 0.3% of hydroxypropyl methylcellulose. The field-amplified sample injection (FASI) technique was applied to the online concentration of the alkaloids. With FASI in the presence of a low conductivity solvent plug (water), an approximately 1,000-fold improvement in sensitivity was achieved without any loss of separation efficiency when compared to conventional sample injection. Under these optimized conditions, a baseline separation of the two analytes was achieved within 16 min and the detection limits for E and PE were 0.7 and 0.6 µg/L, respectively. Without expensive instruments or labeling of the compounds, the limits of detection for E and PE obtained by the proposed method are comparable with (or even lower than) those obtained by capillary electrophoresis laser-induced fluorescence, liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. The method was validated in terms of precision, linearity and accuracy, and successfully applied for the determination of the two alkaloids in Ephedra herbs.

Authors+Show Affiliations

1Engineering Research Center of Chemical Zero Discharge & Department of Chemical Engineering, Chongqing Industry Polytechnic College, Chongqing 401120, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

23619557

Citation

Deng, Dongli, et al. "Determination of Ephedrine and Pseudoephedrine By Field-amplified Sample Injection Capillary Electrophoresis." Journal of Chromatographic Science, vol. 52, no. 4, 2014, pp. 357-62.
Deng D, Deng H, Zhang L, et al. Determination of ephedrine and pseudoephedrine by field-amplified sample injection capillary electrophoresis. J Chromatogr Sci. 2014;52(4):357-62.
Deng, D., Deng, H., Zhang, L., & Su, Y. (2014). Determination of ephedrine and pseudoephedrine by field-amplified sample injection capillary electrophoresis. Journal of Chromatographic Science, 52(4), 357-62. https://doi.org/10.1093/chromsci/bmt039
Deng D, et al. Determination of Ephedrine and Pseudoephedrine By Field-amplified Sample Injection Capillary Electrophoresis. J Chromatogr Sci. 2014;52(4):357-62. PubMed PMID: 23619557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of ephedrine and pseudoephedrine by field-amplified sample injection capillary electrophoresis. AU - Deng,Dongli, AU - Deng,Hao, AU - Zhang,Lichun, AU - Su,Yingying, Y1 - 2013/04/25/ PY - 2013/4/27/entrez PY - 2013/4/27/pubmed PY - 2014/10/31/medline SP - 357 EP - 62 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 52 IS - 4 N2 - A simple and rapid capillary electrophoresis method was developed for the separation and determination of ephedrine (E) and pseudoephedrine (PE) in a buffer solution containing 80 mM of NaH2PO4 (pH 3.0), 15 mM of β-cyclodextrin and 0.3% of hydroxypropyl methylcellulose. The field-amplified sample injection (FASI) technique was applied to the online concentration of the alkaloids. With FASI in the presence of a low conductivity solvent plug (water), an approximately 1,000-fold improvement in sensitivity was achieved without any loss of separation efficiency when compared to conventional sample injection. Under these optimized conditions, a baseline separation of the two analytes was achieved within 16 min and the detection limits for E and PE were 0.7 and 0.6 µg/L, respectively. Without expensive instruments or labeling of the compounds, the limits of detection for E and PE obtained by the proposed method are comparable with (or even lower than) those obtained by capillary electrophoresis laser-induced fluorescence, liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. The method was validated in terms of precision, linearity and accuracy, and successfully applied for the determination of the two alkaloids in Ephedra herbs. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/23619557/Determination_of_ephedrine_and_pseudoephedrine_by_field_amplified_sample_injection_capillary_electrophoresis_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bmt039 DB - PRIME DP - Unbound Medicine ER -